[특허]Methods and systems for image processing of microfluidic devices

[미국특허] Methods and systems for image processing of microfluidic devices 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	G06K-009/36
출원번호	US-0900927 (2007-09-13)
등록번호	US8055034 (2011-10-25)
발명자 / 주소	Dube, Simant Sun, Gang Zhao, Lian-She
출원인 / 주소	Fluidigm Corporation
대리인 / 주소	Kilpatrick Townsend & Stockton LLP
인용정보	피인용 횟수 : 6 인용 특허 : 29

초록 ▼

A method of processing data associated with fluorescent emissions from a microfluidic device. The method includes performing an auto-focus process associated with a first image of the microfluidic device and performing an auto-exposure process associated with the first image of the microfluidic device. The method also includes capturing a plurality of images of the microfluidic device. The plurality of images are associated with a plurality of thermal cycles. The method further includes performing image analysis of the plurality of captured images to determine a series of optical intensities and performing data analysis of the series of optical intensities to provide a series of change in threshold values.

대표청구항 ▼

What is claimed is: 1. A method of processing data associated with fluorescent emissions from a microfluidic device, the method comprising:performing an auto-focus process associated with a first image of the microfluidic device;performing an auto-exposure process associated with the first image of the microfluidic device;capturing a plurality of images of the microfluidic device, wherein the plurality of images are associated with a plurality of thermal cycles;performing image analysis of the plurality of captured images to determine a series of optical intensities; andperforming data analysis of the series of optical intensities to provide a series of changes in threshold values.

이 특허에 인용된 특허 (29) 인용/피인용 타임라인 분석

Sun, Gang; Harris, Greg; May, Andy; Self, Kyle; Farrell, Kevin; Wyatt, Paul, Analysis engine and database for manipulating parameters for fluidic systems on a chip.
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Bashir, Rashid; Gomez, Rafael; Ladisch, Michael R., Biosensor and related method.
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Tom Henry K. (La Honda CA) Rowley Gerald L. (Cupertino CA), Concentrating zone method in heterogeneous immunoassays.
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Söderman, Tobias; Inganäs, Mats, Confidence determination.
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Dale James Langham,AUX ; Timms Peter,AUX ; Walsh Terence Patrick,AUX, Detection of a nucleic acid sequence or a change therein.
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Brow, Mary Ann D.; Hall, Jeff Steven Grotelueschen; Lyamichev, Victor; Olive, David Michael; Prudent, James Robert, Detection of nucleic acid sequences by invader-directed cleavage.
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Ullman Edwin F. (Atherton CA) Schwarzberg Moshe (Palo Alto CA), Fluorescence quenching with immunological pairs in immunoassays.
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Kronick Melvyn N. (Palo Alto CA) Little William A. (Palo Alto CA), Fluorescent immunoassay employing total reflection for activation.
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Taylor, Colin Jon; Sun, Gang; Dube, Simant, Image processing method and system for microfluidic devices.
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Taylor, Colin Jon; Sun, Gang; Dube, Simant, Image processing method and system for microfluidic devices.
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Quake,Stephen R.; Chou,Hou Pu, Integrated active flux microfluidic devices and methods.
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Maggio Edward T. (Redwood City CA), Kit for carrying out chemically induced fluorescence immunoassay.
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Litman David J. (Palo Alto CA) Harel Zvi (Stanford CA) Ullman Edwin F. (Atherton CA), Macromolecular environment control in specific receptor assays.
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Shorte, Spencer; Müller, Torsten; Schnelle, Thomas, Method and device for 3 dimensional imaging of suspended micro-objects providing high-resolution microscopy.
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Quan, Emerson Chueng; Taylor, Colin Jon; Lee, Michael; Cesar, Christopher G.; Harris, Greg; Sun, Gang, Method and system for microfluidic device and imaging thereof.
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Goelet Philip ; Knapp Michael R. ; Anderson Stephen, Method for determining nucleotide identity through extension of immobilized primer.
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Goelet Philip ; Knapp Michael R. ; Anderson Stephen, Method for determining nucleotide identity through primer extension.
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Deutsch,Mordechai; Kaufman,Menachem, Method for identifying an image of a well in an image of a well-bearing component.
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Chait,Arnon; DeLucas,Lawrence; Stoops,Brad; Belgovskiy,Alexander, Method for preparation of microarrays for screening of crystal growth conditions.
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Bajaj S. Paul, Method for the detection of genetic diseases and gene sequence variations by single nucleotide primer extension.
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Eyal Nurit,ILX ; Navot Nir,ILX, Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits t.
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Kwok Pui-Yan ; Chen Xiangning, Methods and kits for nucleic acid analysis using fluorescence resonance energy transfer.
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Kinney,Patrick D.; King,Howard G.; Pallas,Michael C.; Naley,Mark, Methods, software, and apparatus for focusing an optical system using computer image analysis.
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Padmanabhan, Aravind; Fritz, Bernard S., Miniaturized cytometer for detecting multiple species in a sample.
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Foret, Frantisek; Rejtar, Thomas; Zhang, Bailin; Karger, Barry L., Parallel sample loading and injection device for multichannel microfluidic devices.
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Chow,Andrea W., Prevention of precipitate blockage in microfluidic channels.
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Livak Kenneth J. ; Flood Susan J.A. ; Marmaro Jeffrey ; Mullah Khairuzzaman Bashar, Self-quenching fluorescence probe.
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Dunlay, R. Terry; Taylor, D. Lansing, System for cell-based screening.
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Dunlay, R. Terry; Taylor, D. Lansing; Gough, Albert H.; Giuliano, Kenneth A., System for cell-based screening.
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이 특허를 인용한 특허 (6) 인용/피인용 타임라인 분석

Van Leeuwen, Marinus Bastiaan; Vink, Jelte Peter, Method for automatically adjusting a focal plane of a digital pathology image.
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Dube, Simant; Sun, Gang; Zhao, Lian-She, Methods and systems for image processing of microfluidic devices.
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Dube, Simant; Sun, Gang; Zhao, Lian-She, Methods and systems for image processing of microfluidic devices.
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Fu, Haoning, Systems and methods for assessing images.
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Fu, Haoning, Systems and methods for assessing images.
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Mendonca, Paulo Ricardo; Padfield, Dirk Ryan; Aladahalli, Chandan Kumar Mallappa; Liu, Shubao; Broniak, Theresa Rose, Systems and methods for determining parameters for image analysis.
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IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

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[미국특허] Methods and systems for image processing of microfluidic devices 원문보기