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Kafe 바로가기국가/구분 | United States(US) Patent 등록 |
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국제특허분류(IPC7판) |
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출원번호 | US-0569408 (2009-09-29) |
등록번호 | US-8087162 (2012-01-03) |
발명자 / 주소 |
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출원인 / 주소 |
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대리인 / 주소 |
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인용정보 | 피인용 횟수 : 0 인용 특허 : 444 |
A sensor utilizing a non-leachable or diffusible redox mediator is described. The sensor includes a sample chamber to hold a sample in electrolytic contact with a working electrode, and in at least some instances, the sensor also contains a non-leachable or a diffusible second electron transfer agen
A sensor utilizing a non-leachable or diffusible redox mediator is described. The sensor includes a sample chamber to hold a sample in electrolytic contact with a working electrode, and in at least some instances, the sensor also contains a non-leachable or a diffusible second electron transfer agent. The sensor and/or the methods used produce a sensor signal in response to the analyte that can be distinguished from a background signal caused by the mediator. The invention can be used to determine the concentration of a biomolecule, such as glucose or lactate, in a biological fluid, such as blood or serum, using techniques such as coulometry, amperometry; and potentiometry. An enzyme capable of catalyzing the electrooxidation or electroreduction of the biomolecule is typically provided as a second electron transfer agent.
1. A method of manufacturing electrochemical sensors, the method comprising the steps of: forming a plurality of working electrodes on a first region of a first substrate, the working electrodes comprising a material selected from the group consisting of gold, carbon, platinum, ruthenium dioxide, an
1. A method of manufacturing electrochemical sensors, the method comprising the steps of: forming a plurality of working electrodes on a first region of a first substrate, the working electrodes comprising a material selected from the group consisting of gold, carbon, platinum, ruthenium dioxide, and palladium;forming a plurality of counter electrodes on a second region of the first substrate, the counter electrodes comprising a material selected from the group consisting of silver/silver chloride, gold, carbon, platinum, ruthenium dioxide, and palladium;disposing a spacer layer overlaying the working and counter electrodes, the spacer layer having a thickness of no more than 0.2 mm;removing a portion of the spacer layer to define a portion of a boundary for a sample chamber;disposing analyte-responsive enzyme in at least a portion of the sample chamber;overlaying the first substrate and the spacer layer with a second substrate to form a layered structure; andseparating the layered structure into a plurality of electrochemical sensors, each electrochemical sensor comprising: at least one working electrode having a working area of no more than about 0.01 cm2,at least one counter electrode, wherein each at least one working electrode is spaced from each at least one counter electrode by 25-1000 micrometers,at least one sample chamber having a volume less than 1 microliter and including a circular recess having at least a portion of the working electrode and a diffusible redox mediator situated in the recess, andfirst and second lateral extensions from the sensor body flanking the sample chamber region to indicate to a user where the openings for the sample chamber are provided. 2. The method according to claim 1, wherein the step of disposing a spacer layer is done prior to the step of removing a portion of the spacer layer to define a portion of a boundary for a sample chamber. 3. The method according to claim 1, wherein the step of removing a portion of the spacer layer to define a portion of a boundary for a sample chamber is done prior to the step of disposing a spacer layer. 4. The method according to claim 1, wherein the step of disposing a spacer layer comprises: disposing a spacer layer having a thickness of no more than 0.1 mm. 5. The method according to claim 1, further comprising: forming a plurality of indicator electrodes on the substrate; andwherein the step of separating the layered structure into a plurality of electrochemical sensors comprises: separating the layered structure into a plurality of electrochemical sensors, each electrochemical sensor comprising at least one working electrode, at least one counter electrode, at least one indicator electrode, and at least one sample chamber. 6. The method according to claim 1, where the step of forming a plurality of working electrodes comprises: printing a plurality of working electrodes; and wherein the step of forming a plurality of counter electrodes comprises:printing a plurality of counter electrodes. 7. The method according to claim 6, wherein: the step of printing a plurality of working electrodes comprises; printing a carbon ink; andthe step of printing a plurality of counter electrodes comprises: printing a Ag/AgCl ink. 8. The method according to claim 1, wherein the step of separating the layered structure into a plurality of electrochemical sensors comprises: die cutting the layered structure to form the plurality of electrochemical sensors. 9. The method according to claim 1, wherein the step of separating the layered structure into a plurality of electrochemical sensors comprises: slitting the layered structure to form the plurality of electrochemical sensors. 10. The method according to claim 1, wherein the substrate has a length and a width, and wherein: the step of forming a plurality of working electrodes on a first region of the substrate comprises: forming a plurality of working electrodes on the first substrate in the first region having a length and a width; the plurality of working electrodes arranged in columns parallel to the width of the substrate and in rows parallel to the length of the substrate; andthe step of forming a plurality of counter electrodes on a second region of the substrate comprises: forming a plurality of counter electrodes in the second region having a length and a width; the plurality of counter electrodes arranged in columns parallel to the width of the second substrate and in rows parallel to the length of the substrate. 11. The method according to claim 1, fiber comprising: depositing an enzyme over a portion of the working electrodes or the counter electrodes. 12. The method according to claim 11, further comprising: depositing an enzyme continuously over a portion of at least two of the working electrodes or at least two of the counter electrodes. 13. The method according to claim 1, further comprising: depositing a redox mediator over a portion of the working electrodes or the counter electrodes. 14. The method according to claim 13, further comprising: depositing a redox mediator continuously over a portion of at least two of the working electrodes or two of the counter electrodes. 15. The method according to claim 1, wherein the step of forming a plurality of working electrodes comprises: forming a plurality of working electrodes from a carbon ink; and wherein the step of forming a plurality of counter electrodes comprises:forming a plurality of counter electrodes of a Ag/AgCl ink. 16. The method according to claim 1, wherein the step of forming a plurality of working electrodes comprises: forming a plurality of working electrodes from a material comprising gold; and wherein the step of forming a plurality of counter electrodes comprises:forming a plurality of counter electrodes from a material comprising gold. 17. The method according to claim 1, wherein the step of separating the layered structure into a plurality of electrochemical sensors comprises: separating the layered structure into a plurality of electrochemical sensors, each electrochemical sensor comprising: at least one sample chamber having a volume less than 0.5 microliter. 18. The method according to claim 17, wherein the step of separating the layered structure into a plurality of electrochemical sensors comprises: separating the layered structure into a plurality of electrochemical sensors, each electrochemical sensor comprising: at least one sample chamber having a volume less than 0.1 microliter. 19. The method according to claim 1, wherein the step of disposing a spacer layer overlaying the working and counter electrodes comprises: disposing an adhesive spacer layer having a thickness no more than 0.1 mm. 20. A method of manufacturing electrochemical sensors, the method comprising the steps of: forming a plurality of working electrodes on a first region of a first substrate, the working electrodes comprising gold;forming a plurality of counter electrodes on a second region of the first substrate, the counter electrodes comprising gold;disposing a spacer overlaying the working and counter electrodes, the spacer having a thickness of no more than 0.2 mm;removing a portion of the spacer to define a portion of a boundary for a sample chamber;disposing analyte-responsive enzyme in at least a portion of the sample chamber;overlaying the first substrate and the spacer with a second substrate to form a layered structure, wherein the layered structure comprises first and second lateral extensions flanking the sample chamber region to indicate to a user where the openings for the sample chamber are provided; andseparating the layered structure into a plurality of electrochemical sensors, each electrochemical sensor comprising: a sample chamber including at least one working electrode having a working area of no more than about 0.01 cm2 formed within a circular recess therein, the sample chamber including a diffusible redox mediator and having a volume less than 1 microliter; andat least one counter electrode, each at least one working electrode spaced from each at least one counter electrode by 25-1000 micrometers. 21. The method according to claim 20, wherein the step of disposing a spacer is done prior to the step of removing a portion of the spacer. 22. The method according to claim 20, wherein the step of removing a portion of the spacer is done prior to the step of disposing the spacer. 23. The method according to claim 20, wherein the step of disposing a spacer comprises: disposing a spacer having a thickness of no more than 0.1 mm. 24. The method according to claim 20, where the step of forming a plurality of working electrodes comprises: printing a plurality of working electrodes. 25. The method according to claim 20, further comprising: depositing an enzyme over a portion of the working electrodes or the counter electrodes. 26. The method according to claim 20, further comprising: depositing an enzyme continuously over a portion of at least two of the working electrodes or at least two of the counter electrodes. 27. The method according to claim 20, where the sample chamber has a volume of no more than about 0.5 microliters. 28. The method according to claim 27, where the sample chamber has a volume of no more than about 0.25 microliters. 29. The method according to claim 20, wherein the step of separating the layered structure into a plurality of electrochemical sensors comprises: slitting the layered structure.
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