Conditioned cell culture medium compositions and methods of use
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A61K-038/18
C12N-005/02
출원번호
US-0349451
(2009-01-06)
등록번호
US-8138147
(2012-03-20)
발명자
/ 주소
Naughton, Gail K.
Horwitz, David L.
Applegate, Mark A.
Zeltinger, Joan
Mansbridge, Jonathan N.
Kern, Andreas
Landeen, Lee K.
Ratcliffe, Anthony
Pinney, R. Emmett
출원인 / 주소
Skinmedica, Inc.
대리인 / 주소
Wilson, Sonsini, Goodrich & Rosati
인용정보
피인용 횟수 :
8인용 특허 :
43
초록▼
Novel products comprising conditioned cell culture medium compositions and methods of use are described. The conditioned cell medium compositions of the invention may be comprised of any known defined or undefined medium and may be conditioned using any eukaryotic cell type. The medium may be condit
Novel products comprising conditioned cell culture medium compositions and methods of use are described. The conditioned cell medium compositions of the invention may be comprised of any known defined or undefined medium and may be conditioned using any eukaryotic cell type. The medium may be conditioned by stromal cells, parenchymal cells, mesenchymal stem cells, liver reserve cells, neural stem cells, pancreatic stem cells and/or embryonic stem cells. Additionally, the cells may be genetically modified. A three-dimensional tissue construct is preferred. Once the cell medium of the invention is conditioned, it may be used in any state. Physical embodiments of the conditioned medium include, but are not limited to, liquid or solid, frozen, lyophilized or dried into a powder. Additionally, the medium is formulated with a pharmaceutically acceptable carrier as a vehicle for internal administration, applied directly to a food item or product, formulated with a salve or ointment for topical applications, or, for example, made into or added to surgical glue to accelerate healing of sutures following invasive procedures. Also, the medium may be further processed to concentrate or reduce one or more factors or components contained within the medium.
대표청구항▼
1. A method of making a composition, comprising: (a) forming a conditioned medium comprising culturing fibroblasts in a nutrient medium sufficient to meet the nutritional needs required to grow the cells in vitro wherein the conditioned medium comprises at least 3.2 ng/ml vascular endothelial growth
1. A method of making a composition, comprising: (a) forming a conditioned medium comprising culturing fibroblasts in a nutrient medium sufficient to meet the nutritional needs required to grow the cells in vitro wherein the conditioned medium comprises at least 3.2 ng/ml vascular endothelial growth factors (VEGF);(b) removing the conditioned medium from the cultured fibroblasts; and(c) combining the conditioned medium with an acceptable carrier to form the composition. 2. The method of claim 1, wherein the conditioned medium obtained from said cultured fibroblasts further comprises keratinocyte growth factor (KGF). 3. The method of claim 2, wherein said conditioned medium contains at least 1.67 ng/ml KGF. 4. The method of claim 1, wherein culturing comprises a continuous flow system. 5. The method of claim 1, wherein culturing comprises a bioreactor system. 6. The method of claim 1, wherein the cultured fibroblasts are suspended or floated in the nutrient medium. 7. The method of claim 1, further comprising processing said conditioned medium, wherein the conditioned medium in step (b) is concentrated, filtered, purified, or a combination thereof prior to combining with an acceptable carrier in step (c). 8. The method of claim 1, wherein approaching physiological levels is performed by concentrating said conditioned medium. 9. The method of claim 1, wherein said conditioned medium further comprises at least 2.3 ng/ml G-CSF. 10. The method of claim 1, wherein said conditioned medium further comprises at least 0.9 ng/ml IL-8. 11. The method of claim 1, wherein said conditioned medium further comprises at least 0.8 ng/ml TGF-β. 12. A cosmeceutical composition comprising: cell culture media, conditioned by: (a) culturing fibroblasts in a nutrient medium sufficient to meet the nutritional needs required to grow the cells in vitro wherein the conditioned medium comprises at least 3.2 ng/ml vascular endothelial growth factors (VEGF), so that a conditioned medium is formed;(b) removing the conditioned medium from the cultured cells; and(c) combining the conditioned medium with an acceptable carrier to form the composition. 13. The cosmeceutical composition of claim 12, further comprising processing said conditioned medium wherein the conditioned medium is concentrated, filtered, purified, or a combination thereof prior to combining with an acceptable carrier in step (c). 14. The cosmeceutical composition of claim 12, wherein approaching physiological levels is performed by concentrating said conditioned medium. 15. The cosmeceutical composition of claim 12, wherein the conditioned medium obtained from said cultured fibroblasts comprises VEGF and keratinocyte growth factor (KGF). 16. The cosmeceutical composition of claim 12, wherein said composition is an injectable composition or a topical composition. 17. The cosmeceutical composition of claim 16, wherein said topical composition is an ointment, a cream, a hydrogel or a lotion. 18. The conditioned medium of claim 15, wherein the conditioned medium contains at least 1.67 ng/ml KGF. 19. A method of making a composition, comprising: (a) forming a conditioned medium comprising culturing fibroblasts in a nutrient medium sufficient to meet the nutritional needs required to grow the cells in vitro, wherein said cultured fibroblasts form an extracellular matrix and secrete a desired level of extracellular products so that a conditioned medium is formed, wherein at least 3.2 ng/ml of vascular endothelial growth factor (VEGF) is secreted into the conditioned medium;(b) removing the conditioned medium from the cultured fibroblasts; and(c) combining the conditioned medium with an acceptable carrier to form the composition. 20. The method of claim 19, wherein the conditioned medium obtained from said cultured fibroblasts further comprises keratinocyte growth factor (KGF). 21. The method of claim 20, wherein said conditioned medium contains at least 1.67 ng/ml KGF. 22. The method of claim 19, wherein culturing comprises a continuous flow system. 23. The method of claim 19, wherein culturing comprises a bioreactor system. 24. The method of claim 19, wherein the cultured fibroblasts are suspended or floated in the nutrient medium. 25. The method of claim 19, further comprising processing said conditioned medium, wherein the conditioned medium in step (b) is concentrated, filtered, purified, or a combination thereof prior to combining with an acceptable carrier in step (c).
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