Flowcell systems for single molecule detection
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IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C07H-021/04
C12Q-001/68
C12P-019/34
C07F-009/28
출원번호
US-0854137
(2010-08-10)
등록번호
US-8148516
(2012-04-03)
발명자
/ 주소
Williams, John G. K.
Bashford, Gregory R
Chen, Jiyan
Draney, Dan
Narayanan, Nara
Reynolds, Bambi L.
Sheaff, Pamela
출원인 / 주소
Pacific Biosciences of California, Inc.
대리인 / 주소
Kilpatrick Townsend & Stockton LLP
인용정보
피인용 횟수 :
36인용 특허 :
51
초록▼
The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charge-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are dete
The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charge-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation without interference from unincorporated NPs and without illuminating the polymerase-DNA complex.
대표청구항▼
1. A nucleotide, said nucleotide having a detectable label attached to the terminal phosphate, wherein said detectable label is linked to said terminal phosphate with a linker comprising a peptide of 2 to 15 amino acids in length. 2. The nucleotide according to claim 1, wherein said detectable moiet
1. A nucleotide, said nucleotide having a detectable label attached to the terminal phosphate, wherein said detectable label is linked to said terminal phosphate with a linker comprising a peptide of 2 to 15 amino acids in length. 2. The nucleotide according to claim 1, wherein said detectable moiety is selected from the group consisting of chemiluminescent compounds, fluorescent or fluorogenic dyes, colored or chromogenic dyes, electrochemical tags and combinations thereof. 3. The nucleotide of claim 1, wherein said detectable moiety is a fluorogenic dye. 4. The nucleotide of claim 1, wherein said detectable moiety is detectable by a property selected from the group consisting of color, fluorescence emission, chemiluminescence, reduction/oxidation potential and combinations thereof. 5. The nucleotide of claim 4, wherein said detectable moiety is selected from the group consisting of coumarin dyes, xanthene dyes, resorufins, cyanine dyes, difluoroboradiazaindacene dyes (BODIPY), ALEXA dyes, indoles, bimanes, isoindoles, dansyl dyes, naphthalimides, phthalimides, xanthenes, lanthanide dyes, rhodamines and fluoresceins. 6. The nucleotide of claim 4, wherein the said detectable moiety is a member selected from the group consisting of 5-carboxyfluorescein (FAM), rhodamine, 5-(2′-aminoethyl)aminonapthalene-1-sulfonic acid (EDANS), anthranilamide, coumarin, terbium chelate derivatives, Reactive Red 4, BODIPY dyes and cyanine dyes. 7. The nucleotide of claim 1, wherein the sugar moiety of said nucleotide is selected from the group consisting of ribosyl, 2′-deoxyribosyl and other modified sugars. 8. The nucleotide of claim 1, wherein the nucleobase of said nucleotide is selected from the group consisting of uracil, thymine, cytosine, 5-methylcytosine, guanine, 7-deazaguanine, hypoxanthine, 7-deazahypoxanthine, adenine, 7-deazaadenine, 2,6-diaminopurine and analogs thereof. 9. The nucleotide of claim 1, which is a substrate for a nucleic acid polymerase. 10. The nucleotide of claim 1, wherein the detectable moiety after removal of phosphates interacts with additional chemical and/or enzymatic moieties to generate a signal. 11. The nucleotide of claim 10, wherein said additional detection reagents are capable of a response that is detectably different from said detectable species. 12. The nucleotide of claim 1, wherein said peptide is 8 amino acids in length. 13. The nucleotide of claim 12, wherein said nucleotide is compound 55: 14. The nucleotide of claim 1, wherein said peptide is 3 amino acids in length. 15. The nucleotide of claim 14, wherein said peptide of 3 amino acids is KKK. 16. The nucleotide of claim 1, wherein said nucleotide has the formula: wherein: B is a nucleobase;R1 is a hydrogen, a hydroxyl group or charged group;R2 in Formula I is a hydrogen, or charged group;X is a peptide between 2 and 15 amino acids in length;“y” can be 0, 2 or 3; andF is a detectable moiety. 17. A method for detecting the presence of a DNA sequence, said method comprising: a) conducting a DNA polymerase reaction in the presence of a terminal-phosphate-labeled nucleotide of claim 1, which reaction results in the production of a labeled polyphosphate; b) permitting said labeled polyphosphate to react with a phosphate or polyphosphate transferring enzyme to produce a fluorophore moiety; and c) detecting the presence of said fluorophore moiety. 18. The method of claim 17, wherein step (a) further includes conducting said polymerase reaction in the presence of a phosphate or polyphosphate transferring enzyme. 19. The method of claim 17, wherein step (a) further includes conducting said polymerase reaction in the presence of two or more terminal-phosphate-labeled nucleotides with distinct fluorophore moieties. 20. The method of claim 17, wherein said fluorophore moiety is an enzyme-activatable label selected from the group consisting of chemiluminescent compounds, fluorogenic or fluorescent dyes, chromogenic or colored dyes, electrochemical tags and combinations thereof. 21. The method of claim 17, further comprising the step of including one or more additional detection reagents in said polymerase reaction. 22. The method of claim 21, wherein said additional detection reagents are capable of a response that is detectably different from said fluorophore.
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