Flexible membrane valve for cell culture vessel
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
B65D-053/00
C12M-001/24
C12M-003/00
출원번호
US-0148321
(2008-04-18)
등록번호
US-8177082
(2012-05-15)
발명자
/ 주소
Cattadoris, Henry Joseph
Martin, Gregory Roger
Tanner, Allison Jean
출원인 / 주소
Corning Incorporated
대리인 / 주소
Wilks, Susan S.
인용정보
피인용 횟수 :
0인용 특허 :
9
초록▼
An assembly for culturing cells includes a housing defining a reservoir for containing cell culture media and an opening in fluid communication with the reservoir. The cell culture assembly further includes a filter valve assembly configured to sealingly engage the opening. The filter valve assembly
An assembly for culturing cells includes a housing defining a reservoir for containing cell culture media and an opening in fluid communication with the reservoir. The cell culture assembly further includes a filter valve assembly configured to sealingly engage the opening. The filter valve assembly includes a flexible membrane, a contiguous side wall, an end wall, and a microbial filter. The end wall has an opening and extends across one end of the side wall to form a major cavity within the side wall and the end wall. The flexible membrane has a slit spanning the membrane and is disposable within the major cavity such that the membrane sealingly divides the major cavity into (i) a chamber formed between the end wall and the membrane and (ii) a minor cavity formed by the membrane and a portion of the side wall. The minor cavity is in fluid communication with the reservoir. The slit is biased in a closed position to prevent fluid from passing from the minor cavity into the chamber and is configured to open to allow gas to pass from the minor cavity into the chamber when the pressure differential across the membrane is above a threshold. The filter is disposed between the membrane and the opening such that air flowing through the opening in the end wall into the chamber passes through the filter.
대표청구항▼
1. A vent cap assembly for a cell culture apparatus, comprising: a cap housing member having a generally cylindrical side wall and an end wall extending across one end of the side wall to form a major cavity within the cylindrical side wall and the end wall, wherein the end wall has a first major su
1. A vent cap assembly for a cell culture apparatus, comprising: a cap housing member having a generally cylindrical side wall and an end wall extending across one end of the side wall to form a major cavity within the cylindrical side wall and the end wall, wherein the end wall has a first major surface facing the major cavity, an opposing second major surface, and an opening extending through the end wall from the first major surface to the second major surface;a flexible membrane having first and second opposing major surfaces and a slit extending through the membrane from the first major surface to the second major surface, wherein the flexible membrane is disposable within the major cavity such that the membrane sealingly divides the major cavity into (i) a chamber formed between the first major surface of the end wall and the first major surface of the membrane and (ii) a minor cavity formed by the second major surface of the membrane and a portion of the side wall,wherein the slit is biased in a closed position to prevent fluid from passing from the minor cavity into the chamber and is configured to open to allow gas to pass from the minor cavity into the chamber when the pressure differential across the membrane is above a threshold; anda microbial filter disposed between the membrane and the opening such that air flowing through the opening in the end wall into the chamber passes through the filter. 2. The vent cap assembly of claim 1, further comprising a counter bore formed in the first major surface of the end wall and wherein the opening extends through the counter bore. 3. The vent cap assembly of claim 2, wherein the filter is disposed in the counter bore. 4. The vent cap assembly of claim 2, wherein the chamber is formed substantially by the counter bore. 5. The vent cap assembly of claim 1, further comprising a seat extending from the side wall into the cavity, wherein the first major surface of the flexible membrane is configured to sealingly engage the seat. 6. The vent cap assembly of claim 5, wherein the membrane is adhered to the seat. 7. The vent cap assembly of claim 1, further comprising a spacer disposed between the first major surface of the end wall and the first major surface of the membrane. 8. The vent cap assembly of claim 1, wherein the slit is positioned away from the center of the membrane. 9. The vent cap assembly of claim 8, wherein the opening is positioned away from the center of the end wall and is alignable with the slit of the membrane. 10. The vent cap assembly of claim 1, wherein the membrane contains a plurality of slits. 11. The vent cap assembly of claim 10, wherein the slits are positioned away from the center of the membrane and are substantially equally radially spaced apart at a first distance, wherein a counter bore having a diameter greater than the first distance is formed in the first major surface of the end wall, the counter bore being positioned away from the center of the end wall and being alignable with one or more of the slits, andwherein the end wall further comprises a channel formed in the first major surface, wherein the channel provides fluid communication from the counter bore to the opening. 12. A cell culture assembly comprising: a cell culture vessel having a housing defining a reservoir and a neck extending from the housing, the neck defining an opening in fluid communication with the reservoir; andthe vent cap assembly of claim 1, wherein the cap housing member is configured to be disposed about the neck. 13. An assembly for culturing cells, comprising: a housing defining a reservoir for containing cell culture media and an opening in fluid communication with the reservoir; anda filter valve assembly configured to sealingly engage the opening to prevent gas from exiting the reservoir via the opening,wherein the filter valve assembly comprises the flexible membrane, a contiguous side wall, an end wall, and a microbial filter, wherein the end wall extends across one end of the side wall to form a major cavity within the side wall and the end wall,wherein the end wall has a first major surface facing the major cavity, an opposing second major surface, and an opening extending through the end wall from the first major surface to the second major surface,wherein the flexible membrane has first and second opposing major surfaces and a slit spanning the membrane from the first to the second major surface,wherein the flexible membrane is disposable within the major cavity such that the membrane sealingly divides the major cavity into (i) a chamber formed between the first major surface of the end wall and the first major surface of the membrane and (ii) a minor cavity formed by the second major surface of the membrane and a portion of the side wall, the minor cavity being in fluid communication with the reservoir,wherein the slit is biased in a closed position to prevent fluid from passing from the minor cavity into the chamber and is configured to open to allow gas to pass from the minor cavity into the chamber when the pressure differential across the membrane is above a threshold, andwherein the microbial filter is disposed between the membrane and the opening such that air flowing through the opening in the end wall into the chamber passes through the filter. 14. The assembly of claim 13, wherein the filter valve assembly further comprises a seat extending from the side wall into the cavity, wherein the first major surface of the flexible membrane is configured to sealingly engage the seat. 15. The assembly of claim 13, wherein the filter valve assembly further comprises a spacer disposed between the first major surface of the end wall and the first major surface of the membrane. 16. The assembly of claim 13, wherein the housing further comprises a neck, wherein the neck defines the opening. 17. The assembly of claim 16, wherein the filter valve assembly is a vent cap configured to be disposed about the neck. 18. The assembly of claim 13, wherein the side wall of the filter valve assembly is integrally formed with the housing such that the side wall defines the opening. 19. The assembly of claim 13, wherein the housing is gas permeable.
Stephanus Fransiscus Schilthuizen NL; Lukas Wilhelmus van Hees NL, Squeeze bottle for dispensing a liquid in a metered and substantially germ-free manner.
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