초록 ▼

Regulatory regions suitable for directing the expression of a heterologous polynucleotide under light conditions in which the red/far red ratio is less than 1 are described, as well as nucleic acid constructs that include these regulatory regions. Also disclosed are transgenic plants that contain su

Regulatory regions suitable for directing the expression of a heterologous polynucleotide under light conditions in which the red/far red ratio is less than 1 are described, as well as nucleic acid constructs that include these regulatory regions. Also disclosed are transgenic plants that contain such constructs and methods of producing such transgenic plants.

대표청구항 ▼

1. A nucleic acid construct comprising a regulatory region operably linked to a heterologous polynucleotide, said regulatory region having at least 98 percent sequence identity to the polynucleotide sequence set forth in SEQ ID NO: 1, wherein said regulatory region contains a BOXCPSAS1 motif, and wh

1. A nucleic acid construct comprising a regulatory region operably linked to a heterologous polynucleotide, said regulatory region having at least 98 percent sequence identity to the polynucleotide sequence set forth in SEQ ID NO: 1, wherein said regulatory region contains a BOXCPSAS1 motif, and wherein said regulatory region directs transcription of said operably linked heterologous polynucleotide under light conditions in which the red/far red ratio is less than 1. 2. The nucleic acid construct of claim 1, wherein said regulatory region contains a -10PEHVPSBD motif, an ASF1MOTIFCAMV motif, an IBOX motif and an IBOXCORE motif, a CACGTGMOTIF motif, GT1CORE motif, or a REALPHALGLHCB21 motif. 3. The nucleic acid construct of claim 1, wherein said heterologous polynucleotide comprises a nucleotide sequence encoding a polypeptide. 4. The nucleic acid construct of claim 3, wherein said polypeptide is a zinc finger (B-box type) polypeptide. 5. The nucleic acid construct of claim 1, wherein said heterologous polynucleotide is in an antisense orientation relative to said regulatory region. 6. The nucleic acid construct of claim 1, wherein said heterologous polynucleotide is transcribed into an interfering RNA. 7. A transgenic plant or plant cell transformed with the nucleic acid of claim 1. 8. A method of producing a transgenic plant, said method comprising (a) introducing into a plant cell an isolated polynucleotide comprising the nucleic acid construct of claim 1; and (b) growing a plant from said plant cell. 9. A nucleic acid construct comprising a regulatory region operably linked to a heterologous polynucleotide, said regulatory region comprising a 5′ segment having at least 98 percent sequence identity to the polynucleotide sequence set forth in nucleotides 1-1000 of SEQ ID NO: 1, and a 3′ segment comprising a TATA box, wherein said regulatory region contains a BOXCPSAS1 motif, and wherein said regulatory region directs transcription of said operably linked heterologous polynucleotide under light conditions in which the red/far red ratio is less than 1. 10. The nucleic acid construct of claim 9, wherein said heterologous polynucleotide comprises a nucleotide sequence encoding a polypeptide. 11. The nucleic acid construct of claim 10, wherein said polypeptide is a zinc finger (B-box type) polypeptide. 12. The nucleic acid construct of claim 9, wherein said heterologous polynucleotide is transcribed into an interfering RNA. 13. A transgenic plant or plant cell transformed with the nucleic acid of claim 9. 14. A method of producing a transgenic plant, said method comprising (a) introducing into a plant cell an isolated polynucleotide comprising the nucleic acid construct of claim 9; and (b) growing a plant from said plant cell. 15. A nucleic acid construct comprising a regulatory region operably linked to a heterologous polynucleotide, said regulatory region comprising the polynucleotide sequence set forth in SEQ ID NO: 1, wherein said regulatory region directs transcription of said operably linked heterologous polynucleotide under light conditions in which the red/far red ratio is less than 1.