IPC분류정보
국가/구분 |
United States(US) Patent
등록
|
국제특허분류(IPC7판) |
|
출원번호 |
US-0084462
(2006-04-20)
|
등록번호 |
US-8288120
(2012-10-16)
|
국제출원번호 |
PCT/IL2006/000483
(2006-04-20)
|
§371/§102 date |
20080501
(20080501)
|
국제공개번호 |
WO2007/052245
(2007-05-10)
|
발명자
/ 주소 |
|
출원인 / 주소 |
|
인용정보 |
피인용 횟수 :
0 인용 특허 :
97 |
초록
▼
Disclosed is a method of studying floating living cell by confining the cells floating in an aqueous medium in picowells located on a bottom surface of a top wall of a chamber. Disclosed is also a picowell-bearing device for the study of cells comprising a chamber having at least one picowell on a b
Disclosed is a method of studying floating living cell by confining the cells floating in an aqueous medium in picowells located on a bottom surface of a top wall of a chamber. Disclosed is also a picowell-bearing device for the study of cells comprising a chamber having at least one picowell on a bottom surface of the chamber. Also disclosed is a picowell-bearing device for the study of cells comprising a chamber having at least one picowell on an internal surface and at least one moveable wall, a force applicator configured to removeably apply a substantially uniformly distributed force around the periphery of the moveable wall in a direction to retain the moveable wall secured to the chamber.
대표청구항
▼
1. A method of studying living cells having a density that is lower than that of a medium in which the cells are contained, comprising: providing a chamber having a first wall and a second wall, respectively defining top and bottom walls for the chamber, wherein each wall has a first surface inside
1. A method of studying living cells having a density that is lower than that of a medium in which the cells are contained, comprising: providing a chamber having a first wall and a second wall, respectively defining top and bottom walls for the chamber, wherein each wall has a first surface inside the chamber and a second surface outside the chamber and wherein the chamber has one or more picowells a bottom of which is on the first surface of the top wall;loading a quantity of a cell-containing medium containing cells having a density that is lower than that of the medium into the chamber through an opening therein; andallowing the loaded cells to float upwardly toward the first surface of the top wall and thereby to be confined in the picowells; and studying the cells with the chamber positioned so that open ends of the wells face downward. 2. The method of claim 1, wherein said picowells are comprised in a picowell array. 3. The method of claim 1, wherein the cells are confined to the picowells by: a) introducing a medium into said chamber; thenb) introducing a living cell of a first type into said container;c) selecting said medium so that said living cells are less dense than said medium; andd) orienting said chamber so that said bottom surface of said top wall faces substantially downwards,whereby said living cell of said first type floats upwards in said medium to be held in a said picowells for study. 4. The method of claim 3, wherein said medium and said living cell of said first type are introduced through an opening in a bottom wall of said chamber; and, subsequent to said introduction, said opening is substantially closed with a closing component. 5. The method of claim 4, wherein said closing component constitutes a part of a fluid inlet in fluid communication with said chamber. 6. The method of claim 4, further including at least one picowell on said top surface and further comprising: e) introducing a living cell of a second type that is denser than said medium into said picowell on said top surface,whereby, when said chamber is oriented so that said bottom surface of said top wall faces substantially downwards, the top surface of said bottom wall faces substantially upward, and said living cell of said second type settles into a said picowell on said top surface for study. 7. The method of claim 6, wherein said picowell on the top surface of the bottom wall of said chamber is comprised in picowell array. 8. The method of claim 4, wherein said chamber is oriented so that said opening in said bottom wall faces substantially upward while said medium and said living cell of said first type are introduced. 9. The method of claim 1, further including positioning said chamber with its top wall at the bottom and a bottom wall at the top when said medium and said living cells are introduced into said chamber, and inverting said chamber when the cells are to be studied. 10. The method of claim 1, further including orienting the chamber in a loading position wherein the top wall is below the bottom wall before cells are loaded; and closing the opening after the cells have been loaded; andwherein the cells are allowed to float toward the first surface of the top wall by inverting the chamber so that the top wall is above the bottom wall. 11. The method of claim 1, further including introducing at least one active agent into the cavity so that it comes in contact with the contents of the picowells; and studying the cells during or after introduction of the active agent. 12. The method of claim 11, wherein the active agent is introduced into the chamber through a fluid inlet separate from the opening through which the cells are loaded. 13. The method of claim 12, wherein said fluid inlet is a capillary channel. 14. The method of claim 13, wherein said fluid outlet is a capillary channel. 15. The method of claim 14, wherein said chamber includes an excess gas storage area, and the method further includes: orienting the chamber so that gas trapped in the chamber after the medium has been added enters the excess gas storage area; andremoving the excess gas from said chamber through the fluid outlet. 16. The method of claim 11, wherein the active agent is introduced to said picowells through a fluid inlet comprised of a capillary channel defined at least in part by walls of made from a silicone material. 17. The method of claim 16, wherein said fluid inlet includes a hole through said bottom surface of said device body in fluid communication with said capillary channel. 18. The method of claim 16, wherein said silicone material is a silicone rubber. 19. The method of claim 16, wherein two opposing walls of said capillary channel are made out of a silicone rubber. 20. The method of claim 1, further comprising removing fluid from said chamber through at least one fluid outlet in fluid communication with said chamber. 21. The method of claim 1, further including: coupling a gas reservoir to the interior of the chamber; and increasing the content of the gas in the chamber by admitting gas from the gas reservoir. 22. The method of claim 1, wherein, subsequent to loading the cells, the opening through which the cells are introduced is closed by positioning a movable component. 23. The method of claim 22, wherein said movable component constitutes a part of a fluid inlet in fluid communication with the chamber. 24. The method of claim 1, wherein the cells are observed through the top wall of the chamber.
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