Disclosed herein are multi-component peroxycarboxylic acid generation systems for enzymatically producing aqueous formulations of peroxycarboxylic acids suitable for use in, e.g., disinfectant and/or bleaching applications. The multi-component peroxycarboxylic acid generation systems comprise at lea
Disclosed herein are multi-component peroxycarboxylic acid generation systems for enzymatically producing aqueous formulations of peroxycarboxylic acids suitable for use in, e.g., disinfectant and/or bleaching applications. The multi-component peroxycarboxylic acid generation systems comprise at least one carbohydrate esterase family 7 enzyme having perhydrolytic activity.
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1. A multi-component peroxycarboxylic acid generation system comprising a first compartment comprising a first component and a second compartment comprising a second component and means for mixing the first component and the second component to produce an aqueous formulation of peracetic acid; where
1. A multi-component peroxycarboxylic acid generation system comprising a first compartment comprising a first component and a second compartment comprising a second component and means for mixing the first component and the second component to produce an aqueous formulation of peracetic acid; wherein the first component comprises (i) an enzyme powder comprising a formulation of: (a) at least one enzyme catalyst having perhydrolysis activity, wherein said enzyme catalyst comprises an enzyme having a carbohydrate esterase family 7 (CE-7) signature motif that aligns with SEQ ID NO: 1 using CLUSTALW, said signature motif comprising: (1) an RGQ motif at amino acid positions aligning with 118-120 of SEQ ID NO:1;(2) a GXSQG motif at amino acid positions aligning with 179-183 of SEQ ID NO:1; and(3) an HE motif at amino acid positions aligning with 298-299 of SEQ ID NO:1;said enzyme comprising at least 30% amino acid identity to SEQ ID NO: 1; and(b) at least one excipient;(ii) a carboxylic acid ester substrate selected from the group consisting of (a) one or more esters having the structure [X]mR5 whereinX is an ester group of the formula R6C(O)O;R6 is a C1 to C7 linear, branched or cyclic hydrocarbyl moiety, optionally substituted with a hydroxyl group or C1 to C4 alkoxy group, wherein R6 optionally comprises one or more ether linkages where R6 is C2 to C7;R5 is a C1 to C6 linear, branched, or cyclic hydrocarbyl moiety optionally substituted with a hydroxyl group, wherein each carbon atom in R5 individually comprises no more than one hydroxyl group or no more than one ester group, and wherein R5 optionally comprises one or more ether linkages;m is 1 to the number of carbon atoms in R5, said one or more esters having solubility in water of at least 5 ppm at 25° C.;(b) one or more glycerides having the structure wherein R1 is a C1 to C7 straight chain or branched chain alkyl optionally substituted with an hydroxyl or a C1 to C4 alkoxy group and R3 and R4 are individually H or R1C(O);(c) one or more esters of the formula wherein R1 is a C1 to C7 straight chain or branched chain alkyl optionally substituted with an hydroxyl or a C1 to C4 alkoxy group and R2 is a C1 to C10 straight chain or branched chain alkyl, alkenyl, alkynyl, aryl, alkylaryl, alkylheteroaryl, heteroaryl, (CH2CH2O)n, or (CH2CH(CH3)—O)nH and n is 1 to 10;(d) one or more acetylated monosaccharides, acetylated disaccharides, or acetylated polysaccharides; and(e) any combination of (a) through (d);wherein the amount of the carboxylic acid ester substrate in the first component is designed to provide a final concentration of 0.5 wt % to 10 wt % in a reaction formulation formed by combining the first and second components;(iii) a buffer selected from the group consisting of bicarbonate, citrate, acetate, phosphate, pyrophosphate, methylphosphonate, succinate, malate, fumarate, tartrate, and maleate;(iv) a cosolvent selected from the group consisting of tripropylene glycol methyl ether, dipropylene glycol methyl ether, propylene glycol methyl ether, diethylene glycol butyl ether, dipropylene glycol, triethylene glycol, 1,2-propanediol, N-ethyl-2-pyrroldinone, isopropanol, ethanol, ethyl lactate, 1,3-propanediol, and any combination thereof; and(v) optionally at least one surfactant; wherein the second component comprises water, hydrogen peroxide and a hydrogen peroxide stabilizer. 2. The multi-component peroxycarboxylic acid generation system of claim 1 wherein the at least one excipient ranges from about 95 wt % to about 25 wt % of the enzyme powder. 3. The multi-component peroxycarboxylic acid generation system of claim 1 or claim 2 wherein (i) the carboxylic acid ester substrate is triacetin; wherein the amount of triacetin in the first component is designed to provide a final concentration of 0.5 wt % to 10 wt % in a reaction formulation formed by combining the first and second components;(ii) the buffer is in a concentration of about 0.1 wt % to about 10% wt of a the first component and said buffer is selected from the group consisting of sodium bicarbonate, potassium bicarbonate, a mixture of sodium bicarbonate and potassium bicarbonate, sodium phosphate, potassium phosphate, and a mixture of sodium phosphate and potassium phosphate;(iii) the cosolvent is tripropylene glycol methyl ether and is in a concentration of up to 80 wt % of the first component;(iv) the surfactant is present and is polysorbate 80; and(v) the hydrogen peroxide in the second component is present in an amount that provides a final concentration in a reaction formulation formed by combining the first and second components of from 0.33 wt % to about 30 wt %. 4. The multi-component peroxycarboxylic acid generation system of claim 1 or claim 2 wherein the first component is combined with said second component at a ratio of about 1:1 to about 1:10 by weight. 5. The multi-component peroxycarboxylic acid generation system of claim 1 or claim 2 where the multi-component peroxycarboxylic acid generation system is provided in the form of two individual bottles, a two compartment spray bottle, a two compartment packet, or a two-compartment, non-rigid squeeze bottle. 6. A multi-component peroxycarboxylic acid generation system comprising a first compartment comprising a first component and a second compartment comprising a second component and means for mixing the first and second component to produce an aqueous formulation of peracetic acid; wherein the first component comprises (i) an enzyme powder comprising a formulation of (a) at least one CE-7 enzyme having perhydrolysis activity, wherein said at least one CE-7 enzyme comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 19 and SEQ ID NO: 20 or an amino acid sequence having at least 90 % identity to SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 19 or SEQ ID NO: 20; and(b) at least one excipient;(ii) a carboxylic acid ester substrate selected from the group consisting of monoacetin, diacetin, triacetin, and a mixtures thereof; wherein the amount of the carboxylic acid ester substrate in the first component is designed to provide a final concentration of 0.5 wt % to 10 wt% in a reaction formulation formed by combining the first and second components;(iii) a buffer selected from the group consisting of bicarbonate, citrate, acetate, phosphate, pyrophosphate, methylphosphonate, succinate, malate, fumarate, tartrate, and maleate;(iv) a cosolvent selected from the group consisting of tripropylene glycol methyl ether, dipropylene glycol methyl ether, propylene glycol methyl ether, diethylene glycol butyl ether, dipropylene glycol, triethylene glycol, 1,2-propanediol, N-ethyl-2-pyrroldinone, isopropanol, ethanol, ethyl lactate, 1,3-propanediol, and any combination thereof; and(v) optionally at least one surfactant; wherein the second component comprises water, hydrogen peroxide and a hydrogen peroxide stabilizer. 7. The multi-component peroxycarboxylic acid generation system of claim 6 wherein: (i) the at least one CE-7 enzyme comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 19 and SEQ ID NO: 20, wherein amino acid residue 277 of SEQ ID NO: 19 or SEQ ID NO: 20 is selected from the group consisting of alanine, valine, serine, and threonine;(ii) the carboxylic acid ester substrate is triacetin; wherein the amount of triacetin in the first component is designed to provide a final concentration of 0.5 wt % to 10 wt% in a reaction formulation formed by combining the first and second components;(iii) the buffer is in a concentration of about 0.1 wt % to about 10% wt of the first component and said buffer is selected from the group consisting of sodium bicarbonate, potassium bicarbonate, a mixture of sodium bicarbonate and potassium bicarbonate, sodium phosphate, potassium phosphate, and a mixture of sodium phosphate and potassium phosphate;(iv) the cosolvent is tripropylene glycol methyl ether and is in a concentration of up to 80 wt % of the first component;(vi) the surfactant is present and is polysorbate 80; and(vi) the hydrogen peroxide in the second component is present in an amount that provides a final concentration in a reaction formulation formed by combining the first and second components of from 0.33 wt % to about 30 wt %. 8. The multi-component peroxycarboxylic acid generation system of claim 6 or claim 7 wherein the first component is combined with said second component at a ratio of about 1:1 to about 1:10 by weight. 9. The multi-component peroxycarboxylic acid generation system of claim 1 or claim 6 where the multi-component peroxycarboxylic acid generation system is provided in the form of two individual bottles, a two compartment spray bottle, a two compartment packet, or a two-compartment non-rigid squeeze bottle. 10. The multi-component peroxycarboxylic acid generation system of claim 1 or claim 6, further comprising means for applying the aqueous formulation comprising peracetic acid produced by mixing the first and second components to a surface, an article of clothing or a textile for bleaching, stain removal, odor reduction, sanitization, disinfection, or a combination thereof. 11. A method of using the multi-component peroxycarboxylic acid generation system of claim 1 or claim 6 comprising (a) employing the means for mixing the first and second components whereby an aqueous formulation comprising peracetic acid is produced; and(b) applying the aqueous formulation comprising peracetic acid produced in (a) to a surface, an article of clothing or a textile for bleaching, stain removal, odor reduction, sanitization, disinfection, or a combination thereof.
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