Materials and methods for modulating (e.g., increasing or decreasing) lignin content in plants are disclosed. For example, nucleic acids encoding lignin-modulating polypeptides are disclosed as well as methods for using such nucleic acids to generate transgenic plants having a modulated lignin conte
Materials and methods for modulating (e.g., increasing or decreasing) lignin content in plants are disclosed. For example, nucleic acids encoding lignin-modulating polypeptides are disclosed as well as methods for using such nucleic acids to generate transgenic plants having a modulated lignin content.
대표청구항▼
1. A method of increasing lignin content in a plant, said method comprising: a) introducing into a plant cell an isolated nucleic acid comprising a regulatory region operably linked to a polynucleotide encoding a lignin-modulating polypeptide comprising an amino acid sequence having 95% or greater s
1. A method of increasing lignin content in a plant, said method comprising: a) introducing into a plant cell an isolated nucleic acid comprising a regulatory region operably linked to a polynucleotide encoding a lignin-modulating polypeptide comprising an amino acid sequence having 95% or greater sequence identity to the amino acid sequence set forth in SEQ ID NO:149, said polypeptide having a nicotinamide adenine dinucleotide (NAD)-dependent epimerase/dehydratase domain; and b) selecting a plant produced from said plant cell that has at least a 35% increase in lignin content in a tissue of the plant as compared to the lignin content in tissue of a corresponding control plant that does not comprise said nucleic acid. 2. The method of claim 1, wherein said plant cell is capable of producing one or more lignin monomers. 3. The method of claim 1, wherein said plant is from a genus selected from the group consisting of Agrostis, Avena, Festuca, Hordeum, Lolium, Medicago, Milium, Miscanthus, Panicum, Poa, Saccharum, Sorghum, Trifolium, Triticum, and Zea. 4. The method of claim 1, wherein said plant is a species selected from the group consisting of: a Miscanthus×giganteus hybrid, Miscanthus sinensis, Miscanthus sacchariflorus, Panicum virgatum, Populus balsamifera, Sorghum almum, Sorghum halepense, Sorghum vulgare, and Saccharum spp. 5. The method of claim 1, wherein said regulatory region is a promoter. 6. The method of claim 5, wherein said promoter is a lignin or cellulose biosynthesis promoter. 7. The method of claim 6, wherein said promoter is selected from the group consisting of SEQ ID NOs:345-354. 8. The method of claim 5, wherein said promoter is a tissue-preferential promoter. 9. The method of claim 8, wherein said tissue is secondary cell wall, vascular, stem, pith, xylem, phloem, root, tuber, or leaf tissue. 10. The method of claim 5, wherein said promoter is a cell type-preferential promoter. 11. The method of claim 10, wherein said cell is a sieve, laticifer, companion, sclerenchyma, or xylem cell. 12. The method of claim 5, wherein said promoter is an inducible promoter. 13. The method of claim 1, wherein said plant is from a genus selected from the group consisting of Eucalyptus, Oryza, Pinus, Populus, Prunus, and Quercus. 14. The method of claim 1, wherein said method further comprises introducing into said plant cell a nucleic acid encoding a second lignin-modulating polypeptide operably linked to a second regulatory region. 15. The method of claim 14, wherein said nucleic acid encoding a second lignin-modulating polypeptide operably linked to a second regulatory region is present on a second recombinant nucleic acid construct. 16. A plant produced by the method of claim 1. 17. Progeny of the plant of claim 16, wherein said progeny comprises the polynucleotide encoding the lignin-modulating polypeptide comprising an amino acid sequence having 95% or greater identity to the amino acid sequence set forth in SEQ ID NO: 149, said polypeptide having a NAD-dependent epimerase/dehydratase domain, and wherein tissue of said progeny has at least a 35% increase in lignin content as compared to the lignin content in tissue of a corresponding control plant that does not comprise said nucleic acid. 18. The progeny of claim 17, wherein said progeny is from a genus selected from the group consisting of Agrostis, Avena, Festuca, Hordeum, Lolium, Medicago, Milium, Miscanthus, Panicum, Poa, Saccharum, Sorghum, Trifolium, Triticum, and Zea. 19. The progeny of claim 18, wherein said progeny is a species selected from the group consisting of: a Miscanthus×giganteus hybrid, Miscanthus sinensis, Miscanthus sacchariflorus, Panicum virgatum, Populus balsamifera, Sorghum almum, Sorghum halepense, Sorghum vulgare, and Saccharum spp.
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