Polypeptide complex of TRPM8 and calmodulin and its uses thereof
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
G01N-033/53
A61K-038/00
출원번호
US-0590503
(2006-10-31)
등록번호
US-8399201
(2013-03-19)
발명자
/ 주소
Qin, Ning
Flores, Christopher M.
출원인 / 주소
Janssen Pharmaceutica N.V.
대리인 / 주소
Kamage, Andrea Jo
인용정보
피인용 횟수 :
0인용 특허 :
21
초록▼
The present invention discovered that a mammalian TRPM8 binds to calmodulin. The present invention provides a polypeptide complex comprising a cold-menthol receptor (TRPM8) or an active fragment or derivative of TRPM8 and a calmodulin or an active fragment or derivative of calmodulin, and the uses o
The present invention discovered that a mammalian TRPM8 binds to calmodulin. The present invention provides a polypeptide complex comprising a cold-menthol receptor (TRPM8) or an active fragment or derivative of TRPM8 and a calmodulin or an active fragment or derivative of calmodulin, and the uses of the polypeptide complex.
대표청구항▼
1. An isolated polypeptide complex comprising 1) a polypeptide comprising TRPM8 or an active fragment of TRPM8 comprising SEQ ID NO: 9 interacting with 2) calmodulin or an active fragment of calmodulin comprising SEQ ID NO: 1. 2. The polypeptide complex of claim 1, wherein the TRPM8 is associated wi
1. An isolated polypeptide complex comprising 1) a polypeptide comprising TRPM8 or an active fragment of TRPM8 comprising SEQ ID NO: 9 interacting with 2) calmodulin or an active fragment of calmodulin comprising SEQ ID NO: 1. 2. The polypeptide complex of claim 1, wherein the TRPM8 is associated with an isolated membrane. 3. The polypeptide complex of claim 1, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO: 2, SEQ ID NO: 3, or SEQ ID NO: 4. 4. The polypeptide complex of claim 1, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, or SEQ ID NO: 8. 5. The polypeptide complex of claim 1, wherein said calmodulin comprises the amino acid sequence of SEQ ID NO: 1. 6. A method of producing a polypeptide complex of claim 1, comprising the steps of: a. contacting 1) a polypeptide comprising TRPM8 or an active fragment of TRPM8 comprising SEQ ID NO: 9 with 2) calmodulin or an active fragment of calmodulin comprising SEQ ID NO: 1 under a condition that allows the formation of a polypeptide complex comprising said protein of 1) interacting with said protein of 2), wherein at least one of said proteins of 1) and 2) is in isolated form or is recombinantly expressed; andb. isolating said polypeptide complex. 7. The method of claim 6, wherein said TRPM8 is associated with an isolated cell membrane. 8. The method of claim 6, wherein the TRPM8 or the active fragment of TRPM8 is present in a cell. 9. The method of claim 8, wherein the cell is a cultured neuron. 10. The method of claim 8, wherein the cell recombinantly expresses the TRPM8 or the active fragment of TRPM8. 11. The method of claim 10, wherein the cell comprises an expression vector having a nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 9. 12. The method of claim 6, wherein the TRPM8 or the active fragment of TRPM8 is in isolated form. 13. The method of claim 6, wherein the calmodulin is in isolated form. 14. The method of claim 6, wherein the calmodulin is present in a cell. 15. The method of claim 14, wherein the cell recombinantly expresses the calmodulin. 16. The method of claim 15, wherein the cell comprises an expression vector having a nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 1. 17. An isolated active fragment of TRPM8 consisting of the amino acid sequence of SEQ ID NO: 9. 18. The polypeptide complex of claim 3, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO: 2. 19. The polypeptide complex of claim 18, wherein said polypeptide consists of the amino acid sequence of SEQ ID NO: 2. 20. The polypeptide complex of claim 3, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO: 3. 21. The polypeptide complex of claim 20, wherein said polypeptide consists of the amino acid sequence of SEQ ID NO: 3. 22. The polypeptide complex of claim 3, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO: 4. 23. The polypeptide complex of claim 22, wherein said polypeptide consists of the amino acid sequence of SEQ ID NO: 4. 24. The polypeptide complex of claim 1, wherein said polypeptide consists of the amino acid sequence of SEQ ID NO: 9. 25. The polypeptide complex of claim 4, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO: 8. 26. The polypeptide complex of claim 25, wherein said polypeptide consists of the amino acid sequence of SEQ ID NO: 8. 27. The polypeptide complex of claim 4, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO: 7. 28. The polypeptide complex of claim 27, wherein said polypeptide consists of the amino acid sequence of SEQ ID NO: 7. 29. The polypeptide complex of claim 4, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO: 6. 30. The polypeptide complex of claim 29, wherein said polypeptide consists of the amino acid sequence of SEQ ID NO: 6. 31. The polypeptide complex of claim 4, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO: 5. 32. The polypeptide complex of claim 31, wherein said polypeptide consists of the amino acid sequence of SEQ ID NO: 5.
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