Bee venom may be administered in a standardized formulation with or without relatively small amounts of anesthetic. In particular, the results of the combination of venom and anesthetic dramatically decreased pain and discomfort for patients undergoing apitherapy.
대표청구항▼
1. A standardized bee venom preparation suitable for administration by injection prepared by a process comprising (a) dissolving pure dried bee venom crystals in a liquid carrier to obtain a bee venom preparation in a concentration of between about 0.1 milligram (mg) to about 10.0 mg by weight of be
1. A standardized bee venom preparation suitable for administration by injection prepared by a process comprising (a) dissolving pure dried bee venom crystals in a liquid carrier to obtain a bee venom preparation in a concentration of between about 0.1 milligram (mg) to about 10.0 mg by weight of bee venom per milliliter (mL) of said bee venom preparation, (b) purifying said bee venom preparation through a filter to obtain a standardized bee venom preparation, wherein said standardized bee venom preparation comprises between about 0.1 mg to about 1.0 mg of pure melittin and about 400 microgram (mcg) to about 4,500 mcg of total protein per mL of said standardized bee venom preparation, wherein said standardized bee venom preparation exhibits 40 to 100 honey bee hyaluronidase units per mL (HHU/mL) of hyaluronidase activity when diluted to 100 mcg/mL and is capable of inhibiting gelatin induced aggregation of erythrocytes of 3-5 millimeter per hour (mm/H). 2. The standardized bee venom preparation of claim 1, wherein said filter is a 25 micrometer or finer filter. 3. The standardized bee venom preparation of claim 1, wherein said liquid carrier comprises sterile deoinized water or physiological saline solution. 4. The standardized bee venom preparation of claim 1, further comprising an excipient selected from the group consisting of a viscosity modifier, preservative, additive, sodium chloride, saccharide, benzyl alcohol, and methyl paraban. 5. The standardized bee venom preparation of claim 1, further comprising admixing an anesthetic with said standardized bee venom preparation. 6. The standardized bee venom preparation of claim 5, wherein said anesthetic is selected from the group consisting of ambucaine, amolanone, amylocaine hydrochloride, benoxinate, benzocaine, betoxycaine, biphenamine, bupivacaine, butacaine, butamben, butanilicaine, butethamine, butoxycaine, carticaine, chloroprocaine hydrochloride, cocaethylene, cocaine, cyclomethycaine, dibucaine hydrochloride, dimethisoquin, dimethocaine, diperodon hydrochloride, dyclonine, ecgonidine, ecgonine, ethyl chloride, etidocaine, β-eucaine, euprocin, fenalcomine, fomocaine, hexylcaine hydrochloride, hydroxytetracaine, isobutyl p-aminobenzoate, leucinocaine mesylate, levoxadrol, lidocaine, mepivacaine, meprylcaine, metabutoxycaine, methyl chloride, myrtecaine, naepaine, octacaine, orthocaine, oxethazaine, parethoxycaine, phenacaine hydrochloride, phenol, piperocaine, piridocaine, polidocanol, plidocanol, pramoxine, prilocaine, procaine, propanocaine, proparacaine, propipocaine, propoxycaine hydrochloride, pseudococaine, pyrrocaine, ropivacaine, salicyl alcohol, tetracaine hydrochloride, tolycaine, trimecaine, and zolamine. 7. The standardized bee venom preparation of claim 5, wherein said anesthetic is lidocaine. 8. The standardized bee venom preparation of claim 5, wherein the amount of said anesthetic provided is in a ratio of between about 20:1 to about 1:10 by weight relative to the weight of said standardized bee venom preparation. 9. The standardized bee venom preparation of claim 8, wherein the amount of said anesthetic provided is in a ratio of between 10:1 to about 1:5 by weight relative to the weight of said standardized bee venom preparation. 10. The standardized bee venom preparation of claim 9, wherein the amount of said anesthetic provided is in a ratio of between 3:1 to about 1:1 by weight relative to the weight of said standardized bee venom preparation. 11. The standardized bee venom preparation of claim 1, wherein said liquid carrier is water or a physiological saline solution. 12. The standardized bee venom preparation of claim 1, further comprising a conventional excipient selected from the group consisting of a viscosity modifier, a preservative, an additive and a mixture thereof. 13. The standardized bee venom preparation of claim 12, wherein said conventional excipient is sodium chloride, saccharide, benzyl alcohol, or methylparaban. 14. The standardized bee venom preparation of claim 1, wherein said pure dried bee venom crystals are collected by a process comprising: (i) placing an extractor in front of a bee hive, wherein said extractor comprises an inner and an outer frame, said outer frame comprising wires that can be alternately grounded and charged, and said inner frame comprising a stretched silicone rubber sheet, wherein said inner frame is placed in said outer frame such that a bee in contact with two consecutive wires can force its stinger into said silicone rubber sheet; (ii) applying an electric current to said wires of said extractor, wherein said electric current is alternatively on and off; (iii) removing said inner frame from said extractor; and (iv) collecting pure dried bee venom crystals from said inner frame.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (6)
Robson Charles H. (6241 S. 30th St. Phoenix AZ 85040), Bee venom collection apparatus.
Dhaliwal Avtar S. (Ste. 43 ; 408 State of Franklin Rd. Johnson City TN 37604) Masih Shabir Z. (Ste. 43 ; 408 State of Franklin Rd. Johnson City TN 37604), Composite anesthetic article and method of use.
Tsay Yuh-Geng (San Jose CA) Beigler Myron A. (Los Altos Hills CA) Calenoff Emanuel (Burlingame CA) Friesen Gerald L. (Vacaville CA) Nichols James L. (Los Altos CA), Stable allergenic extracts and methods.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.