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Kafe 바로가기국가/구분 | United States(US) Patent 등록 |
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국제특허분류(IPC7판) |
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출원번호 | US-0910460 (2004-08-03) |
등록번호 | US-8486720 (2013-07-16) |
발명자 / 주소 |
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출원인 / 주소 |
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대리인 / 주소 |
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인용정보 | 피인용 횟수 : 4 인용 특허 : 463 |
The present invention provides a method for the generation of novel libraries of encoded magnetic particles from sub-libraries of by the generation of novel sub-libraries of magnetic nanoparticles and encoded particles. The sub-libraries are functionalized on demand are useful in the formation of ar
The present invention provides a method for the generation of novel libraries of encoded magnetic particles from sub-libraries of by the generation of novel sub-libraries of magnetic nanoparticles and encoded particles. The sub-libraries are functionalized on demand are useful in the formation of arrays. The present invention is especially useful for performing multiplexed (parallel) assays for qualitative and/or quantitative analysis of binding interactions of a number of analyte molecules in a sample.
1. A method of forming an array, comprising: providing a plurality of magnetic particles comprising at least two different particle populations, each bearing a different binding agent and being distinguishable by an optically differentiable code associated therewith, suspended in a fluid; contacting
1. A method of forming an array, comprising: providing a plurality of magnetic particles comprising at least two different particle populations, each bearing a different binding agent and being distinguishable by an optically differentiable code associated therewith, suspended in a fluid; contacting the magnetic particles with analytes in solution under conditions permitting the binding agents to bind to analytes in an analyte-specific manner;assembling the magnetic particles, following said contacting and binding to analytes, in a planar array on one or more chips using a magnetic field; and then forming a gel film on the surface of the chips and immobilizing the magnetic particles on the chips by embedding them in the gel film. 2. The method of claim 1 wherein the particles are randomly placed in the array. 3. The method of claim 1 wherein a plurality of arrays are assembled on a plurality of chips. 4. The method of claim 2 wherein the chips include microwells patterned on the chips. 5. The method of claim 1 wherein the gel film is a polymer.
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