[특허]Detection system for high sensitivity fluorescent assays

[미국특허] Detection system for high sensitivity fluorescent assays 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	C12M-001/34 G01N-033/544
출원번호	US-0659576 (2012-10-24)
등록번호	US-8492139 (2013-07-23)
발명자 / 주소	Tan, Hong Zuk, Robert F. Tan, Yushan Cao, Erhua Xia, Min Chen, Jun
출원인 / 주소	Access Medical Systems, Ltd.
대리인 / 주소	Perkins Coie LLP
인용정보	피인용 횟수 : 0 인용 특허 : 26

초록 ▼

This invention relates to a detection system for measuring a fluorescent signal in a fluorescent assay. The system comprises a probe having a small sensing surface bound with a fluorescent label, and a light source and a detector both mounted at the proximal side of the sensing surface of the substrate. The invention also relates to a method for detecting an analyte in a liquid sample using a probe tip having a small surface area (≦5 mm) and a high molecular weight polymer (≧1 MD) having multiple binding molecules and multiple fluorescent labels. The binding reaction is accelerated by flowing the reaction solutions laterally and moving the probe tip up and down in the reaction vessels. The invention furthers relates to a fluorescent labeling composition comprising a cross-linked FICOLL® molecule having a plurality of binding molecules and a plurality of fluorescent labels.

대표청구항 ▼

1. A detection system for measuring a fluorescent signal in a fluorescent assay, comprising: a probe having an aspect ratio of length to width at least 5 to 1, the probe having a distal end and a proximal end, the proximal end having a sensing surface bound with a fluorescent label wherein the fluorescent label comprises a polysaccharide having a molecular weight of at least 1 million Daltons and conjugated with at least 5 binding molecules and at least 25 fluorescent labels;a light source for emitting excitation light directly to the probe's sensing surface;a collecting lens pointed toward the sensing surface; andan optical detector for detecting the emission fluorescent light; where the collecting lens collects and directs the emission fluorescent light to the optical detector. 2. The system according to claim 1, wherein the probe sensing surface has a diameter equal or less than 5 mm. 3. The system according to claim 1, wherein the probe is transparent. 4. The system according to claim 3, wherein the distal end of the probe is tapered and/or roughened to reduce undesired reflection. 5. The system according to claim 3, wherein the light source is aligned so that the light beam projected onto the sensing surface at an incident angle greater than the numerical aperture angle of the probe. 6. The system according to claim 3, wherein the probe is made of glass, quartz, or plastic. 7. The system according to claim 1, wherein the probe is a non-transparent monolithic rod. 8. The system according to claim 1, wherein the probe is made of plastic, ceramic, or metal. 9. The system according to claim 1, wherein the sensing surface is coated with a film selected from aluminum, gold, or silver, the film has a thickness from about 50 nm to about 500 μm. 10. The system according to claim 9, wherein a second film of SiO2 is coated on top of the film of claim 9. 11. The system according to claim 1, further comprising a vessel containing a solution, wherein the proximal end of the probe is immersed in the solution, and the light source and the collecting lens are mounted on the bottom side of the vessel.

이 특허에 인용된 특허 (26) 인용/피인용 타임라인 분석

Eibl Johann (Vienna ATX) Aicher Helmut (Obersiebenbrunn ATX), Apparatus for performing a radioimmunological method of determining antigens or antibodies.
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Hubscher Thomas T. (18912 Glendower Rd. Gaithersburg MD 20879), Apparatus for performing determinations of immune reactants in biological fluids.
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Khler Dora (Kreutzer Weg 11 D-1000 Berlin 45 DEX), Carrier device for immunological assay.
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Walt, David R.; Barnard, Steven M., Fiber optic array sensors, apparatus, and methods for concurrently visualizing and chemically detecting multiple analytes of interest in a fluid sample.
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Walt David R. (Lexington MA) Bernard Steven M. (Basel CHX), Fiber optic sensor, apparatus, and methods for detecting an organic analyte in a fluid or vapor sample.
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Walt David R. (Lexington MA), Fiber optic sensors, apparatus, and detection methods using controlled release polymers and reagent formulations held wi.
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Ullman Edwin F. (Atherton CA) Schwarzberg Moshe (Palo Alto CA), Fluorescence quenching with immunological pairs in immunoassays.
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Hirschfeld Tomas E. (Livermore CA), Fluorescent immunoassay employing optical fiber in capillary tube.
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Zuk Robert F. (Burlingame CA) Choo Sae Hyun (Cupertino CA), Fluorescent labelling compositions and methods for their use.
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Pinkel Daniel ; Gray Joe ; Albertson Donna G., High density array fabrication and readout method for a fiber optic biosensor.
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Walt David R. (Lexington MA) Barnard Steven M. (Medford MA), Imaging fiber optic array sensors, apparatus, and methods for concurrently detecting multiple analytes of interest in a.
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Azad A. R. M. (Stoughton MA) Kirchanski Stefan J. (Framingham MA) Brown Michael C. (Wayland MA), Immunological reagents employing polymeric backbone possessing reactive functional groups.
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Zuk Robert F. (Mountain View CA) Maggio Edward T. (Redwood City CA), Label modified immunoassays.
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Noack William L. (Camarillo CA), Liquid removal device.
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Smith Kendall O. (133 Trillium La. San Antonio TX 78213) Gehle Warren D. (6815 Forest Haven San Antonio TX 78240), Magnetic attraction transfer process for use in solid phase radioimmunoassays and in other assay methods.
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Waggoner Alan S. (Pittsburgh PA) Ernst Lauren A. (Pittsburgh PA) Mujumdar Ratnakar B. (Glenshaw PA), Method for labeling and detecting materials employing arylsulfonate cyanine dyes.
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El Shami A. Said (Agoura Hills CA) Alaba Olusola O. (Pasadena CA) Kasal Charles A. (South Pasadena CA), Method for measuring antigens or antibodies in biological fluids using ligand labeled antigens or ligand labeled antibod.
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Walt David R. (Lexington MA) Barnard Steven M. (Medford MA), Method of making imaging fiber optic sensors to concurrently detect multiple analytes of interest in a fluid sample.
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Hubscher Thomas T. (18912 Glendower Rd. Gaithersburg MD 20879), Methods for performing determinations of immune reactants in biological fluids.
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Terasaki Paul I. (Los Angeles CA) Aoki Dennis (Los Angeles CA), Microdroplet test apparatus.
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Kobayashi Takeshi (Nagoya JPX) Iijima Shinji (Nagoya JPX) Sakai Yasunori (Gifu JPX) Shimada Ken-ichi (Gifu JPX), Optical fiber based fluorescent immunoassay apparatus.
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Walt David R. ; Taylor Laura, Optical fiber biosensor array comprising cell populations confined to microcavities.
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Walt, David R.; Taylor, Laura, Optical fiber biosensor array comprising cell populations confined to microcavities.
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Walt David R. ; Michael Karri L ; Chadha Suneet, Optical sensor apparatus for far-field viewing and making optical analytical measurements at remote locations.
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Alfrey Clarence P. (Houston TX) Bedford Donald B. (New Braunfels TX), Radioimmunoassay apparatus.
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Tuunanen Jukka,FIX, Solid phase immunoassay with carriers matching the shape of sample wells.
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[미국특허] Detection system for high sensitivity fluorescent assays 원문보기

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[미국특허] Detection system for high sensitivity fluorescent assays 원문보기

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