초록 ▼

Methods are disclosed for reducing the shift in EMF bias in a magnesium ion selective electrode, comprising the step of: contacting the electrode with a composition comprising a polyoxyalkylene nonionic surfactant; wherein the polyoxyalkylene nonionic surfactant has an HLB greater than about 18. Add

Methods are disclosed for reducing the shift in EMF bias in a magnesium ion selective electrode, comprising the step of: contacting the electrode with a composition comprising a polyoxyalkylene nonionic surfactant; wherein the polyoxyalkylene nonionic surfactant has an HLB greater than about 18. Additional aspects of the present invention are directed to methods, comprising the steps of: contacting a magnesium ion selective electrode with a composition comprising a polyoxyalkylene nonionic surfactant, wherein the polyoxyalkylene nonionic surfactant has an HLB greater than about 18; and measuring a biologically relevant level of a blood electrolyte in a blood composition with the electrode. In certain embodiments, the polyoxyalkylene nonionic surfactant is polyoxy ethylene (100) stearyl ether and the blood electrolyte is magnesium ion.

대표청구항 ▼

1. A method of reducing the shift in electromotive force (EMF) bias in a magnesium ion selective electrode, comprising the step of: contacting said electrode with a composition comprising a polyoxyalkylene nonionic surfactant;wherein said polyoxyalkylene nonionic surfactant has a hydrophilic-lipophi

1. A method of reducing the shift in electromotive force (EMF) bias in a magnesium ion selective electrode, comprising the step of: contacting said electrode with a composition comprising a polyoxyalkylene nonionic surfactant;wherein said polyoxyalkylene nonionic surfactant has a hydrophilic-lipophilic balance (HLB) greater than about 18. 2. The method of claim 1, wherein said shift in EMF bias is less than about 10 mV. 3. The method of claim 1, wherein said polyoxyalkylene nonionic surfactant is a polyoxyethylene nonionic surfactant. 4. The method of claim 3, wherein said polyoxyethylene nonionic surfactant is a polyoxyethylene stearyl ether surfactant having between about 90 and about 110 oxyethylene units. 5. The method of claim 4, wherein said polyoxyethylene stearyl ether surfactant having between about 90 and about 110 oxyethylene units is a polyoxyethylene (100) stearyl ether surfactant. 6. The method of claim 1, wherein the concentration of said polyoxyalkylene nonionic surfactant is between about 0.010 g/L and about 2.20 g/L. 7. The method of claim 6, wherein the concentration of said polyoxyalkylene nonionic surfactant is between about 0.010 g/L and about 1.00 g/L. 8. The method of claim 7, wherein the concentration of said polyoxyalkylene nonionic surfactant is between about 0.010 g/L and about 0.50 g/L. 9. The method of claim 8, wherein the concentration of said polyoxyalkylene nonionic surfactant is between about 0.010 g/L and about 0.25 g/L. 10. The method of claim 9, wherein the concentration of said polyoxyalkylene nonionic surfactant is between about 0.010 g/L and about 0.075 g/L. 11. The method of claim 10, wherein the concentration of said polyoxyalkylene nonionic surfactant is between about 0.010 g/L and about 0.050 g/L. 12. A method, comprising the steps of: contacting a magnesium ion selective electrode with a composition comprising a polyoxyalkylene nonionic surfactant,wherein said polyoxyalkylene nonionic surfactant has a hydrophilic-lipophilic balance (HLB) greater than about 18; andmeasuring a biologically relevant level of magnesium ion in a blood composition with said electrode. 13. The method of claim 12, wherein said polyoxyalkylene nonionic surfactant causes less than about a 10 mV shift in EMF bias in said magnesium ion selective electrode. 14. The method of claim 12, wherein said polyoxyalkylene nonionic surfactant is a polyoxyethylene nonionic surfactant. 15. The method of claim 14, wherein said polyoxyethylene nonionic surfactant is a polyoxyethylene stearyl ether surfactant having between about 90 and about 110 oxyethylene units. 16. The method of claim 15, wherein said polyoxyethylene stearyl ether surfactant having between about 90 and about 110 oxyethylene units is a polyoxyethylene (100) stearyl ether surfactant. 17. The method of claim 12, wherein said blood composition is whole blood, serum, or plasma. 18. The method of claim 12, wherein said biologically relevant level of said blood electrolyte is between about 0.1 mM and about 1.0 mM. 19. The method of claim 18, wherein said biologically relevant level of said blood electrolyte is between about 0.3 mM and about 0.7 mM. 20. The method of claim 12, wherein the concentration of said polyoxyalkylene nonionic surfactant is between about 0.010 g/L and about 2.20 g/L. 21. The method of claim 20, wherein the concentration of said polyoxyalkylene nonionic surfactant is between about 0.010 g/L and about 1.00 g/L. 22. The method of claim 21, wherein the concentration of said polyoxyalkylene nonionic surfactant is between about 0.010 g/L and about 0.50 g/L. 23. The method of claim 22, wherein the concentration of said polyoxyalkylene nonionic surfactant is between about 0.010 g/L and about 0.25 g/L. 24. The method of claim 23, wherein the concentration of said polyoxyalkylene nonionic surfactant is between about 0.010 g/L and about 0.075 g/L. 25. The method of claim 24, wherein the concentration of said polyoxyalkylene nonionic surfactant is between about 0.010 g/L and about 0.050 g/L. 26. A kit comprising: a magnesium ion selective electrode;a calibration reagent comprising a polyoxyalkylene nonionic surfactant,wherein said polyoxyalkylene nonionic surfactant has a hydrophilic-lipophilic balance (HLB) greater than about 18;a wash reagent comprising a polyoxyalkylene nonionic surfactant,wherein said polyoxyalkylene nonionic surfactant has a hydrophilic-lipophilic balance (HLB) greater than about 18;a slope reagent comprising a polyoxyalkylene nonionic surfactant,wherein said polyoxyalkylene nonionic surfactant has a hydrophilic-lipophilic balance (HLB) greater than about 18; andinstructions for rinsing, calibrating, and operating the electrode. 27. The kit of claim 26, wherein said polyoxyalkylene nonionic surfactant is a polyoxyethylene nonionic surfactant. 28. The kit of claim 27, wherein said polyoxyethylene nonionic surfactant is a polyoxyethylene stearyl ether surfactant having between about 90 and about 110 oxyethylene units. 29. The kit of claim 28, wherein said polyoxyethylene stearyl ether surfactant having between about 90 and about 110 oxyethylene units is a polyoxyethylene (100) stearyl ether surfactant. 30. The kit of claim 26, wherein the concentration of said polyoxyalkylene nonionic surfactant is between about 0.010 g/L and about 2.20 g/L. 31. The kit of claim 30, wherein the concentration of said polyoxyalkylene nonionic surfactant is between about 0.010 g/L and about 1.00 g/L. 32. The kit of claim 31, wherein the concentration of said polyoxyalkylene nonionic surfactant is between about 0.010 g/L and about 0.50 g/L. 33. The kit of claim 32, wherein the concentration of said polyoxyalkylene nonionic surfactant is between about 0.010 g/L and about 0.25 g/L. 34. The kit of claim 33, wherein the concentration of said polyoxyalkylene nonionic surfactant is between about 0.010 g/L and about 0.075 g/L. 35. The kit of claim 34, wherein the concentration of said polyoxyalkylene nonionic surfactant is between about 0.010 g/L and about 0.050 g/L.