초록 ▼

A fibrin wound dressing is made by mixing quantities of fibrinogen solution and thrombin solution with air. The resulting foam is very light weight, and with the proper attention to the amount of thrombin, will rest on a vertical surface without dripping. The wound dressing may also be formulated fo

A fibrin wound dressing is made by mixing quantities of fibrinogen solution and thrombin solution with air. The resulting foam is very light weight, and with the proper attention to the amount of thrombin, will rest on a vertical surface without dripping. The wound dressing may also be formulated for its ability to continue migration of healing substances, such as PDGF, from the dressing to the wound site. Thrombin substitutes, such as other clotting proteins, may be used instead of thrombin. The resulting foam may also be lyophilized or ground and lyophilized for later reconstitution. A therapeutic drug or other additive may also be added to the wound dressing. A fibrin foam may be made by injecting fibrinogen foam into a clotting protein, such as thrombin.

대표청구항 ▼

1. A method of forming a fibrin foam, the method comprising: preparing a fibrinogen foam by mixing air with a solution of fibrinogen using a mixer comprising a sintered polymeric material forming a three-dimensional lattice defining a plurality of tortuous interconnecting passages therethrough, the

1. A method of forming a fibrin foam, the method comprising: preparing a fibrinogen foam by mixing air with a solution of fibrinogen using a mixer comprising a sintered polymeric material forming a three-dimensional lattice defining a plurality of tortuous interconnecting passages therethrough, the mixer having physical characteristics to sufficiently mix the components; andinjecting the fibrinogen foam into a solution of thrombin to form a fibrin foam. 2. The method of claim 1, further comprising applying the fibrin foam to a wound surface, and optionally, wherein the fibrin foam has a viscosity sufficient for a thin coating of the fibrin foam to adhere to a vertical surface. 3. The method of claim 1, wherein a rate of formation of the fibrin foam is constant in a temperature range from about 15° C. to about 37° C. 4. The method of claim 1, wherein the fibrinogen foam is prepared by mixing in a mixing device with at least two chambers having an inline mixer therebetween. 5. The method of claim 1, further comprising inhibiting the forming of the fibrin foam by adding an effective amount of a photosensitive inhibitor to the solution of thrombin. 6. The method of claim 1, further comprising lyophilizing the foam to form a lyophilisate, and optionally grinding the foam or the lyophilisate into a powder. 7. The method of claim 6, further comprising reconstituting the fibrin foam by mixing with water or a water solution and optionally adding an additional component. 8. The method of claim 1, further comprising selecting a container for holding the solution of thrombin and receiving the fibrinogen foam, wherein the fibrin foam takes a shape of the container. 9. A method of forming a fibrin foam, the method comprising: preparing a fibrinogen foam by mixing air with a solution of fibrinogen using a mixer comprising a sintered polymeric material forming a three-dimensional lattice defining a plurality of tortuous, interconnecting passages therethrough, the mixer having physical characteristics to sufficiently mix the components in a hand-held mixing device; andinjecting the fibrinogen foam into a solution of thrombin to form a fibrin foam. 10. The method of claim 9, wherein the fibrin foam comprises at least fifty percent voids by volume, and optionally, wherein the fibrin foam is an open cell foam. 11. The method of claim 9, wherein the solution of fibrinogen or the solution of thrombin includes an effective amount of an additive wherein particles of the additive are sized between one micrometer and one nanometer in a major dimension of the particles. 12. The method of claim 9, wherein the fibrinogen is made from a solution having a concentration from about 1 mg/ml to about 110 mg/ml fibrinogen and wherein the thrombin is made from a solution of thrombin having a concentration from about 0.01 IU/ml to about 500 IU/ml. 13. The method of claim 9, further comprising a therapeutic amount of an agent, a drug, an antibiotic, a free growth factor, or a bioactive fusion protein in the solution of fibrinogen or in the solution of thrombin. 14. The method of claim 9, wherein the fibrinogen further comprises from about 10 to about 1000 μg PDGF, PTH or BMP per ml. 15. The method of claim 9, wherein the fibrinogen further comprises from about 10 to about 1000 μg free growth factor or bioactive fusion protein. 16. A method for forming a fibrin foam, the method comprising: preparing a fibrinogen foam by mixing a fibrinogen solution and air using a mixer comprising a sintered polymeric material forming a three-dimensional lattice defining a plurality of tortuous, interconnecting passages therethrough, the mixer having physical characteristics to sufficiently mix the components; andinjecting the foam into a solution of a clotting protein to form a fibrin foam. 17. The method of claim 16, wherein the clotting protein is selected from the group consisting of thrombin, a PTC-derived thrombin substitute, and a p-aminoaniline-based protein. 18. The method of claim 16, further comprising applying the fibrin foam to a wound of a patient. 19. The method of claim 16, further comprising selecting a container for holding the solution of the clotting protein, wherein the fibrin foam takes the shape of the container upon forming. 20. The method of claim 16, further comprising mixing at least one of the solutions with a therapeutic amount of a calcium-containing compound, an agent, a drug, an antibiotic, or a hormone. 21. The method of claim 16, wherein at least the step of preparing takes place in a hand-held mixing device. 22. The method of claim 16, wherein at least the step of preparing takes place in a hand-held mixing device having at least two chambers with an inline mixer therebetween. 23. The method of claim 16, wherein the fibrinogen solution or the solution of the clotting protein further comprises an additive. 24. A method of forming a fibrin foam, the method comprising: preparing a fibrinogen foam by mixing air with a solution of fibrinogen using a mixer comprising a sintered material forming a three-dimensional lattice defining a plurality of tortuous interconnecting passages therethrough, the mixer having physical characteristics to sufficiently mix the components, the sintered material comprising one of glass, ceramic and metal; andinjecting the fibrinogen foam into a solution of thrombin to form a fibrin foam. 25. The method of claim 24, further comprising applying the fibrin foam to a wound surface, and optionally, wherein the fibrin foam has a viscosity sufficient for a thin coating of the fibrin foam to adhere to a vertical surface. 26. The method of claim 24, wherein a rate of formation of the fibrin foam is constant in a temperature range from about 15° C. to about 37° C. 27. The method of claim 24, wherein the fibrinogen foam is prepared by mixing in a mixing device with at least two chambers having the inline mixer therebetween. 28. The method of claim 24, further comprising inhibiting the forming of the fibrin foam by adding an effective amount of a photosensitive inhibitor to the solution of thrombin. 29. The method of claim 24, further comprising lyophilizing the foam to form a lyophilisate, and optionally grinding the foam or the lyophilisate into a powder. 30. The method of claim 29, further comprising reconstituting the fibrin foam by mixing with water or a water solution and optionally adding an additional component. 31. The method of claim 24, further comprising selecting a container for holding the solution of thrombin and receiving the fibrinogen foam, wherein the fibrin foam takes a shape of the container.