IPC분류정보
국가/구분 |
United States(US) Patent
등록
|
국제특허분류(IPC7판) |
|
출원번호 |
US-0148844
(2010-02-11)
|
등록번호 |
US-8530202
(2013-09-10)
|
우선권정보 |
JP-2009-029185 (2009-02-11) |
국제출원번호 |
PCT/JP2010/052001
(2010-02-11)
|
§371/§102 date |
20110810
(20110810)
|
국제공개번호 |
WO2010/092997
(2010-08-19)
|
발명자
/ 주소 |
- Isono, Naoto
- Yamamoto, Yutaka
- Saburi, Wataru
|
출원인 / 주소 |
|
대리인 / 주소 |
|
인용정보 |
피인용 횟수 :
1 인용 특허 :
0 |
초록
▼
A method for manufacturing a linear-chain beta-1,3-glucan is disclosed that comprises polymerizing glucose-1-phosphate serving as a substrate by contacting the glucose-1-phosphate with a beta-1,3-glucan phosphorylase derived from a species in the genus Ochromonas. A laminarioligosaccharide may be ad
A method for manufacturing a linear-chain beta-1,3-glucan is disclosed that comprises polymerizing glucose-1-phosphate serving as a substrate by contacting the glucose-1-phosphate with a beta-1,3-glucan phosphorylase derived from a species in the genus Ochromonas. A laminarioligosaccharide may be added to serve as a primer. A linear-chain beta-1,3-glucan having a degree of polymerization between about 30 to 70 is also disclosed.
대표청구항
▼
1. A method for manufacturing a linear-chain beta-1,3-glucan, comprising: polymerizing glucose-1-phosphate serving as a substrate by contacting the glucose-1-phosphate with a beta-1,3-glucan phosphorylase derived from a species in the genus Ochromonas and with a laminarioligosaccharide serving as a
1. A method for manufacturing a linear-chain beta-1,3-glucan, comprising: polymerizing glucose-1-phosphate serving as a substrate by contacting the glucose-1-phosphate with a beta-1,3-glucan phosphorylase derived from a species in the genus Ochromonas and with a laminarioligosaccharide serving as a primer, thereby forming the linear-chain beta-1,3-glucan. 2. The method according to claim 1, wherein the beta-1,3-glucan phosphorylase is derived from Ochromonas danica. 3. The method according to claim 1, wherein the pH during the polymerization reaction is between 4 and 8. 4. The method according to claim 1, wherein the concentration of glucose-1-phosphate during the polymerization reaction is between 0.01 M and 0.6 M. 5. The method according to claim 1, wherein the temperature during the polymerization reaction is between 20° C. and 45° C. 6. The method according to claim 5, wherein the beta-1,3-glucan phosphorylase is derived from Ochromonas danica, the pH during the polymerization reaction is between 4 and 8, and the concentration of glucose-1-phosphate during the polymerization reaction is between 0.01 M and 0.6 M. 7. The method according to claim 6, wherein the linear beta-1,3-glucan has a degree of polymerization between 30 to 70. 8. The method according to claim 1, wherein the linear beta-1,3-glucan has a degree of polymerization between 30 to 70. 9. The method according to claim 7, wherein the laminarioligosaccharide has a degree of polymerization between 2 and 20. 10. The method according to claim 7, wherein the laminarioligosaccharide is laminaribiose or laminaripentaose. 11. The method according to claim 9, wherein the initial concentration of laminarioligosaccharide is between 0.1 and 2 mg/mL. 12. The method according to claim 11, wherein the initial pH during the polymerization reaction is between 5.1 and 7.3 and the concentration of glucose-1-phosphate is between 0.1 M and 0.5 M. 13. The method according to claim 1, wherein the laminarioligosaccharide has a degree of polymerization between 2 and 20. 14. The method according to claim 1, wherein the laminarioligosaccharide is laminaribiose or laminaripentaose. 15. A method for manufacturing a linear-chain beta-1,3-glucan containing only beta-1,3-bonds, comprising: polymerizing glucose-1-phosphate serving as a substrate by contacting the glucose-1-phosphate with a beta-1,3-glucan phosphorylase derived from a species in the genus Ochromonas and with a laminarioligosaccharide containing only beta-1,3-bonds and serving as a primer, thereby forming the linear-chain beta-1,3-glucan containing only beta-1,3-bonds. 16. The method according to claim 15, wherein the laminarioligosaccharide has a degree of polymerization between 2 and 20 and the linear beta-1,3-glucan has a degree of polymerization between 30 to 70. 17. The method according to claim 16, wherein the beta-1,3-glucan phosphorylase is derived from Ochromonas danica. 18. The method according to claim 17, wherein the laminarioligosaccharide is laminaribiose or laminaripentaose.
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