IPC분류정보
국가/구분 |
United States(US) Patent
등록
|
국제특허분류(IPC7판) |
|
출원번호 |
US-0024422
(2011-02-10)
|
등록번호 |
US-8603835
(2013-12-10)
|
발명자
/ 주소 |
|
출원인 / 주소 |
- Chembio Diagnostic Systems, Inc.
|
대리인 / 주소 |
|
인용정보 |
피인용 횟수 :
15 인용 특허 :
310 |
초록
▼
Test cells have a first sorbent strip with a sample receiving location and defining a first migration path, a distinct second sorbent strip which receives buffer solution and at least partially defines a second migration path distinct from and elongated relative to the first migration path, conjugat
Test cells have a first sorbent strip with a sample receiving location and defining a first migration path, a distinct second sorbent strip which receives buffer solution and at least partially defines a second migration path distinct from and elongated relative to the first migration path, conjugate supported by the second strip, a test site located at a junction of the first and second strips and having an immobilized ligand-binding mechanism, and a divider which directs a first amount of the buffer to the first strip to move the sample to the test site and a second amount to the second strip to move the conjugate to the test site. The first and second migration paths have first and second lengths chosen so that ligand in the sample reaches the test site and binds to the immobilized ligand-binding mechanism prior to the conjugate reaching the test site.
대표청구항
▼
1. A test device for determining the presence of a ligand in a sample, comprising: a) buffer solution;b) a first sorbent strip having a first location for receiving a sample and defining a first migration path;c) a second sorbent strip distinct from said first sorbent strip, said second sorbent stri
1. A test device for determining the presence of a ligand in a sample, comprising: a) buffer solution;b) a first sorbent strip having a first location for receiving a sample and defining a first migration path;c) a second sorbent strip distinct from said first sorbent strip, said second sorbent strip having a second location for receiving said buffer solution and at least partially defining a second migration path distinct from said first migration path and elongated relative to said first migration path;d) a conjugate supported by said second sorbent strip;e) a test site located on or in at least one of said first sorbent strip and said second sorbent strip, said test site having an immobilized ligand-binding mechanism, and said first and second sorbent strips touching each other at the test site;f) a frangible container and divider located at said second location, said frangible container containing said buffer solution, said divider directing a first amount of said buffer solution to said first sorbent strip to move said sample to said test site and a second amount to said second sorbent strip to move said conjugate to said test site upon breaking of said frangible container; andg) a housing defining a first opening adjacent said first location, a second opening adjacent said second location, and a window adjacent said test site through which said test site is viewable, wherein said first migration path is provided with a first length and said second migration path is provided with a second length, and said first length said second length are chosen to permit said sample to reach said test site so that ligand in said sample reaches said test site and binds to said immobilized ligand-binding mechanism prior to said conjugate reaching said test site. 2. A test device according to claim 1, wherein: said second migration path is tortuous. 3. A test device according to claim 2, wherein: said second migration path is at least four times as long as said first migration path. 4. A test device according to claim 1, wherein: said second sorbent strip includes a delay element. 5. A test device according to claim 1, further comprising: a third sorbent strip distinct from said first sorbent strip and said second sorbent strip, said third sorbent strip coupling said second location to said second sorbent strip, said third sorbent strip having slower flow characteristics than said first sorbent material. 6. A test device according to claim 1, wherein: said conjugate comprises an antigen or antibody for the ligand and a marker coupled to the antigen or antibody. 7. A test device according to claim 6, wherein: said marker is a colored marker viewable in the visible spectrum. 8. A test device according to claim 1, wherein: said at least one of said first and second sorbent strips includes a control site, and said housing defines a window for viewing said control site. 9. A test device according to claim 1, wherein: said first sorbent strip has a first pore size and said second sorbent strip has a second pore size, and said first pore size is larger than said second pore size. 10. A test device according to claim 9, wherein: said first pore size is between 3 and 20 microns, andsaid second pore size is between 20 and 40 microns. 11. A test device according to claim 1, wherein: said divider comprises a wedge or a wall. 12. A test device according to claim 1, wherein: said second location is adjacent said first location. 13. A method for testing a sample for the presence of a ligand, comprising: a) obtaining a test device according to claim 1;b) applying the sample to said first location;c) after said applying the sample, applying the buffer solution to the second location by breaking the frangible container; andd) inspecting said test site to determine an indication the presence or lack thereof of the ligand in the sample. 14. A test device for determining the presence of a ligand in a sample, said test device for use with a buffer solution and comprising: a) a first sorbent or bibulous material having a first location for receiving a sample and defining a first horizontal migration path for the sample;b) a second sorbent or bibulous material in fluid contact with said first location and defining a second horizontal migration path for the sample;c) a third sorbent or bibulous material distinct from said first and second sorbent or bibulous materials, said third sorbent or bibulous material receiving the buffer solution and at least partially defining a third horizontal migration path distinct from said first and second migration paths and elongated relative to said first and second migration paths;d) a first conjugate supported by said third sorbent or bibulous material;e) a fourth sorbent or bibulous material distinct from said first and second sorbent or bibulous materials, said fourth sorbent or bibulous material receiving the buffer solution and at least partially defining a fourth horizontal migration path distinct from said first and second migration paths and elongated relative to said first and second migration paths;f) a second conjugate supported by said fourth sorbent or bibulous material;g) a first test site located on or in at least one of said first sorbent or bibulous material and said third sorbent or bibulous material, said first test site having a first immobilized ligand-binding mechanism for detecting antibodies in the sample, and said first and third sorbent or bibulous materials touching each other at said first test site;h) a second test site located on or in at least one of said second sorbent or bibulous material and said fourth sorbent or bibulous material, said second test site having a second immobilized ligand-binding mechanism for detecting antigens in the sample, said second and fourth sorbent or bibulous materials touching each other at said second test site; andi) a divider which directs a first amount of said buffer solution to said first and second sorbent or bibulous materials to move said sample to said first and second test sites and a second amount of said buffer solution to said third and fourth sorbent or bibulous materials to move said first and second conjugates to said first and second test sites, wherein said first migration path is provided with a first length, said second migration path is provided with a second length, said third migration path is provided with a third length, and said fourth migration path is provided with a fourth length, and said first length and said third length are chosen to permit said sample to reach said first test site so that ligand in said sample reaches said first test site and binds to said first immobilized ligand-binding mechanism prior to said first conjugate reaching said first test site, and said second length and said fourth length are chosen to permit said sample to reach said second test site so that ligand in said sample reaches said second test site and binds to said second immobilized ligand-binding mechanism prior to said second conjugate reaching said second test site. 15. A test device according to claim 14, further comprising: a fifth sorbent or bibulous material distinct from said first, second, third and fourth sorbent or bibulous materials, said third fifth sorbent or bibulous material coupling said second location to said third sorbent or bibulous material, said fifth sorbent or bibulous material having slower flow characteristics than said first sorbent or bibulous material. 16. A test device according to claim 15, further comprising: a sixth sorbent or bibulous material distinct from said first, second, third and fourth sorbent or bibulous strips, said sixth sorbent or bibulous material coupling said second location to said third sorbent or bibulous strip, said fifth sorbent or bibulous material having slower flow characteristics than said second sorbent or bibulous material. 17. A test device according to claim 16, wherein: said fifth and sixth sorbent or bibulous materials have portions which are not distinct from each other. 18. A method according for testing a sample for the presence ligands, comprising: a) obtaining a test device according to claim 14;b) applying the sample to said first location;c) after said applying the sample, applying the buffer solution to the second location; andd) inspecting said first and second test sites to determine an indication the presence or lack thereof of the ligands in the sample. 19. A method according to claim 18, wherein: said test device has a housing defining a first opening adjacent said first location, a second opening adjacent said second location, a first window adjacent said first test site through which said first test site is viewable, and a second window adjacent said second test site through which said second test site is viewable;said applying the sample comprises depositing the sample through said first opening to said first location,said applying the solution comprises depositing the buffer solution through said second opening to said second location, andsaid inspecting comprises inspecting through said first window and through said second window.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.