Lipoprotein analysis by differential charged-particle mobility
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
G01N-033/92
G01N-001/00
C07K-001/30
출원번호
US-0589404
(2012-08-20)
등록번호
US-8709818
(2014-04-29)
발명자
/ 주소
Caulfield, Michael P.
Reitz, Richard E.
Li, Shuguang
Lee, Gloria Kwangja
Krauss, Ronald
Blanche, Patricia J.
Benner, W. Henry
Cornell, Earl
출원인 / 주소
Quest Diagnostics Investments Incorporated
대리인 / 주소
Foley & Lardner LLP
인용정보
피인용 횟수 :
0인용 특허 :
27
초록▼
The invention provides methods of preparation of lipoproteins from a biological sample, including HDL, LDL, Lp(a), IDL, and VLDL, for diagnostic purposes utilizing differential charged particle mobility analysis methods. Further provided are methods for analyzing the size distribution of lipoprotein
The invention provides methods of preparation of lipoproteins from a biological sample, including HDL, LDL, Lp(a), IDL, and VLDL, for diagnostic purposes utilizing differential charged particle mobility analysis methods. Further provided are methods for analyzing the size distribution of lipoproteins by differential charged particle mobility, which lipoproteins are prepared by methods of the invention. Further provided are methods for assessing lipid-related health risk, cardiovascular condition, risk of cardiovascular disease, and responsiveness to a therapeutic intervention, which methods utilize lipoprotein size distributions determined by methods of the invention.
대표청구항▼
1. A method for analyzing the size distribution of lipoproteins, said method not including centrifugation, said method comprising: a) admixing a solution comprising lipoproteins and non-lipoproteins with one or more polyanionic compounds and one or more divalent cations;b) allowing a precipitate con
1. A method for analyzing the size distribution of lipoproteins, said method not including centrifugation, said method comprising: a) admixing a solution comprising lipoproteins and non-lipoproteins with one or more polyanionic compounds and one or more divalent cations;b) allowing a precipitate containing lipoproteins to form in said admixed solution;c) after step b), collecting the precipitated lipoproteins; andd) subjecting said one or more lipoproteins to differential charged particle mobility analysis, said analysis comprising: (i) determining a particle size distribution in one or more regions of particle sizes;(ii) subtracting contribution to the particle size distribution of a non-lipoprotein reagent or a non-lipoprotein sample material to obtain a lipoprotein particle size distribution; and(iii) outputting the lipoprotein particle size distribution to a display, a printer or a memorythereby determining the size distribution of said lipoproteins,wherein the determining a particle size distribution includes determining a best fit for the one or more regions. 2. The method of claim 1, wherein said polyanionic compound is selected from the group consisting of dextran sulfate, amylopectin, and polyvinyl sulfate, and wherein said divalent cation is selected from the group consisting of Mg2+ and Ca2+. 3. The method according to claim 1, wherein said one or more lipoproteins are from a plasma sample obtained from an individual. 4. The method according to claim 3, further comprising: (e) using said determined lipoproteins size distribution to conduct an assessment of said individual, said assessment selected from the group consisting of lipid-related health risk, cardiovascular condition, risk of cardiovascular disease, and responsiveness to a therapeutic intervention. 5. The method according to claim 1, wherein said one or more lipoproteins are selected from the group consisting of HDL, LDL, Lp(a), IDL and VLDL. 6. The method according to claim 1, wherein the best fit is of the form: y1=k1*e(−0.7*d);where y1 is a contribution to the measured differential mobility size distribution, k1 is an empirical constant of the fit, and d is particle diameter;wherein the determining a best fit includes calculating a value for k1. 7. The method according to claim 1, wherein the subtracting includes applying a theoretical curve representing particle sizedistribution of the non-lipoprotein reagent or the non-lipoprotein sample material. 8. The method according to claim 1, wherein the non-lipoprotein sample material is albumin. 9. A method for analyzing the size distribution of lipoproteins, said method comprising: a) admixing a solution comprising lipoproteins and non-lipoproteins with one or more polyanionic compounds and one or more divalent cations;b) allowing a precipitate containing lipoproteins to form in said admixed solution;c) collecting the precipitated lipoproteins;d) subjecting said one or more lipoproteins to differential charged particle mobility analysis, which analysis comprises: (i) determining a particle size distribution in one or more regions of particle sizes;(ii) subtracting contribution to the particle size distribution of a non-lipoprotein reagent or a non-lipoprotein sample material to obtain a lipoprotein particle size distribution; and(iii) outputting the lipoprotein particle size distribution to a display, a printer or a memorythereby determining the size distribution of said lipoproteins,wherein said method does not include centrifugation and wherein the non-lipoprotein reagent is Reactive Green 19 (RG 19) conjugated with dextran.
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