Highly efficient gas permeable devices and methods for culturing cells
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12M-001/00
C12M-003/00
C12M-001/04
C12M-001/24
출원번호
US-0952848
(2007-12-07)
등록번호
US-8809044
(2014-08-19)
발명자
/ 주소
Wilson, John R.
출원인 / 주소
Wilson Wolf Manufacturing Corporation
대리인 / 주소
Wintrhop & Weinstine, P.A.
인용정보
피인용 횟수 :
3인용 특허 :
73
초록▼
This invention relates to methods and devices that improve cell culture efficiency. They include the use of gas permeable culture compartments that reduce the use of space while maintaining uniform culture conditions, and are more suitable for automated liquid handling. They include the integration
This invention relates to methods and devices that improve cell culture efficiency. They include the use of gas permeable culture compartments that reduce the use of space while maintaining uniform culture conditions, and are more suitable for automated liquid handling. They include the integration of gas permeable materials into the traditional multiple shelf format to resolve the problem of non-uniform culture conditions. They include culture devices that use surfaces comprised of gas permeable, plasma charged silicone and can integrate traditional attachment surfaces, such as those comprised of traditional tissue culture treated polystyrene. They include culture devices that integrate gas permeable, liquid permeable membranes. A variety of benefits accrue, including more optimal culture conditions during scale up and more efficient use of inventory space, incubator space, and disposal space. Furthermore, labor and contamination risk are reduced.
대표청구항▼
1. A method of culturing animal cells in a gas permeable multi-shelf cell culture apparatus, the method comprising: adding animal cells and media into a gas permeable multi-shelf apparatus comprising two or more culture compartments, each compartment including a shelf comprised of gas permeable, liq
1. A method of culturing animal cells in a gas permeable multi-shelf cell culture apparatus, the method comprising: adding animal cells and media into a gas permeable multi-shelf apparatus comprising two or more culture compartments, each compartment including a shelf comprised of gas permeable, liquid impermeable material for cells to reside upon, each shelf connected to an opposing surface, a fluid pathway shared by said culture compartments, and each said shelf is in contact with a gas space,whereby said apparatus is incubated in the presence of ambient gas suitable for animal cell culture, oriented in a position such that said culture compartments are located one above the other, each said shelf is in a horizontal position with said gas space located below it, animal cells reside upon at least a portion of each said shelf, said culture compartments include media in contact with said shelf and said opposing surface, and ambient gas resides within each said gas space and is in contact with each shelf. 2. The method of claim 1 wherein a culture compartment support is in contact with a portion of each said shelf. 3. The method of claim 2 wherein said culture compartment support includes projections that make contact with said shelf. 4. The method of claim 3 wherein said projections are dispersed throughout said gas space. 5. The method of claim 1 wherein each said shelf is parallel to its said opposing surface. 6. The method of claim 1 wherein the ratio of the volume of media within said apparatus to the surface area of said gas permeable, liquid impermeable walls is in the range of 0.2 ml/cm2 to 0.4 ml/cm2. 7. A method of culturing animal cells, the method comprising: adding animal cells and media into a gas permeable multi-shelf apparatus comprising two or more culture compartments, each having a gas permeable, liquid impermeable wall for cells to reside upon and an opposing wall, a portion of each said gas permeable, liquid impermeable wall in contact with a culture compartment support, a manifold that creates a fluid pathway between said culture compartments, at least a portion of each said gas permeable, liquid impermeable wall is in contact with a gas space, andculturing said animal cells while said apparatus resides in the presence of ambient gas suitable for animal cell culture and in a position wherein said culture compartments reside at differing elevations, said culture compartments are full of media, each said gas permeable, liquid impermeable wall is held in a horizontal position by said culture compartment support, animal cells reside upon at least a portion of each gas permeable, liquid impermeable wall, said ambient gas suitable for animal cell culture resides in each said gas space and makes contact with each said gas permeable, liquid impermeable wall. 8. The method of claim 7 wherein said culture compartment support includes projections that make contact with said gas permeable, liquid impermeable wall. 9. The method of claim 8 wherein said projections are dispersed throughout said gas space. 10. The method of claim 7 wherein each said gas permeable, liquid impermeable wall is parallel to its said opposing wall. 11. The method of claim 7 wherein the ratio of the volume of media within said apparatus to the surface area of said gas permeable, liquid impermeable material is in the range of 0.2 ml/cm2 to 0.4 ml/cm2. 12. A static method of culturing animal cells, the method comprising: adding animal cells and media into a gas permeable multi-shelf cell culture apparatus comprised of more than one culture space, each culture space including a shelf comprised of gas permeable, liquid impermeable material for cells to reside upon, each shelf connected to an opposing surface that is oriented in parallel to the shelf, each culture space is connected to a common fluid pathway, a gas space in contact with at least a portion of said gas permeable, liquid impermeable material of each shelf, a shelf support structure in contact with a portion of each said shelf and acting to maintain the shelf in a planar state, andculturing said animal cells when said cell culture apparatus is in a location that includes ambient gas suitable for animal cell culture and said cell culture apparatus is in a position wherein each said shelf is in a horizontal position and a gas space is located below each said shelf, said shelves oriented one above the other, the space between each shelf and its opposing surface includes media in contact with the shelf and its opposing surface, and ambient gas is in the gas space located below each shelf. 13. The method of claim 12 wherein said shelf support structure includes projections that make contact with said shelf. 14. The method of claim 13 wherein said projections are dispersed throughout said gas space. 15. The method of claim 12 wherein the ratio of the volume of media within said apparatus to the surface area of said shelves is in the range of 0.2 ml/cm2 to 0.4 ml/cm2. 16. A method of culturing cells, the method comprising: adding animal cells and media into a gas permeable multi-shelf apparatus comprising more than one culture compartment, each having a gas permeable, liquid impermeable shelf and an opposing wall, a gas space in communication with each said gas permeable, liquid impermeable shelf, and a manifold providing said culture compartments a shared fluid pathway, andplacing said apparatus in contact with ambient gas suitable for cell culture in a position such that said culture compartments are oriented one above the other, media is in a static state, each said gas permeable, liquid impermeable shelf resides in a horizontal position with its opposing wall residing above it and ambient gas makes contact with at least a portion of the underside of each said gas permeable, liquid impermeable shelf. 17. The method of claim 16 wherein a portion of each said gas permeable, liquid impermeable shelf is in contact with a culture compartment support. 18. The method of claim 17 wherein said culture compartment support includes projections that make contact with said gas permeable, liquid impermeable shelf. 19. The method of claim 18 wherein said projections are dispersed throughout said gas space. 20. The method of claim 16 wherein each said gas permeable, liquid impermeable shelf is parallel to its said opposing wall. 21. The method of claim 20 wherein the ratio of the volume of media within said apparatus to the surface area of said gas permeable, liquid impermeable shelves is in the range of 0.2 ml/cm2 to 0.4 ml/cm2. 22. A method of culturing cells, the method comprising: adding animal cells and media into a gas permeable multi-shelf apparatus comprising more than one cell compartment, each cell compartment having a shelf for cells to reside upon comprised of a gas permeable, liquid impermeable membrane connected to an opposing wall, a manifold connecting said cell compartments, each said shelf in contact with projections that hold the shelf a planar position, said projections allowing ambient gas to make contact with each said shelf,whereby the animal cells are cultured within said apparatus when said apparatus is in contact with ambient gas suitable for animal cell culture and in a position wherein each said shelf is horizontal, said shelves at differing elevations, ambient gas is in contact with the underside of each said shelf, each said cell compartment is full of media, and the animal cells reside upon each said shelf. 23. The method of claim 22 wherein each said shelf is parallel to its said opposing wall. 24. The method of claim 22 wherein the ratio of the volume of media within said apparatus to the surface area of said shelves is in the range of 0.2 ml/cm2 to 0.4 ml/cm2. 25. A method of culturing animal cells, the method comprising: adding animal cells and media into a gas permeable multi-shelf apparatus comprising two or more culture spaces, each space having a gas permeable, liquid impermeable surface for cells to reside upon and an opposing surface, projections acting to maintain said gas permeable, liquid impermeable surface in a planar position so that it can be oriented in a horizontal position with said projections acting to allow ambient gas to make contact with the underside said gas permeable, liquid impermeable surface, a fluid pathway in communication with each said culture space, andculturing said animal cells while said apparatus is in contact with ambient gas suitable for animal cell culture, the orientation of said apparatus being such that each said gas permeable, liquid impermeable surface resides at a different elevation than the other said gas permeable, liquid impermeable surfaces, said culture spaces are full of media that is in a static state, each said gas permeable, liquid impermeable surface is in a horizontal position, and ambient gas is in contact with at least a portion of the underside of each said gas permeable, liquid impermeable surface. 26. The method of claim 25 wherein each said gas, permeable liquid impermeable surface is parallel to its said opposing surface. 27. The method of claim 25 wherein the ratio of the volume of media within said apparatus to the surface area of said gas permeable, liquid impermeable surfaces is in the range of 0.2 ml/cm2 to 0.4 ml/cm2. 28. A method of culturing cells comprising: incubating cells in the presence of ambient gas suitable for cell culture, said cells residing in a gas permeable multi-shelf cell culture apparatus wherein said cells reside upon more than one shelf, each shelf comprised of gas permeable, liquid impermeable material, each shelf connected by a fluid pathway that is integral to said apparatus, a portion of the bottom of each shelf is in contact with a shelf support that maintains the shelf in a horizontal position, said shelves located at differing elevations, said ambient gas is in contact with the bottom of each said shelf, each said shelf has an opposing surface connected to it and residing above it, and media is in static contact with each shelf and its opposing surface. 29. The method of claim 28 wherein each said shelf support includes projections that are in contact with said gas permeable shelf. 30. The method of claim 29 wherein each said gas permeable shelf is parallel to its said opposing surface. 31. The method of claim 29 wherein each said gas permeable shelf is rectangular. 32. The method of claim 28 wherein the ratio of the volume of media within said apparatus to the surface area of said shelves is in the range of 0.2 ml/cm2 to 0.4 ml/cm2. 33. A method of culturing cells consisting essentially of: adding cells and media into a gas permeable, liquid impermeable cell culture apparatus; andincubating said apparatus in contact with ambient gas suitable for cell culture,wherein said cells reside upon more than one shelf, each shelf comprised of gas permeable, liquid impermeable material, a portion of the bottom of each said shelf is in contact with a shelf support that maintains the shelf in a horizontal position and at a different elevation relative to other shelves, said ambient gas is in contact with the bottom of each said shelf, each said shelf is connected to an opposing surface residing above it, the space between each said shelf and its opposing surface is full of media, and the space between each said shelf and its opposing surface is in communication with a common fluid pathway. 34. The method of claim 33 wherein each said shelf support includes projections that make contact with said shelf. 35. The method of claim 33 wherein said common fluid pathway is full of said media. 36. The method of claim 33 wherein said ambient gas suitable for cell culture is located within a cell culture incubator that includes carbon dioxide. 37. The method of claim 33 wherein said shelf is parallel to its opposing surface. 38. The method of claim 33 wherein the ratio of the volume of media within said apparatus to the surface area of said shelves is in the range of 0.2 ml/cm2 to 0.4 ml/cm2. 39. A method of culturing cells, the method comprising: adding animal cells and media into a gas permeable multi-shelf apparatus comprising more than one culture compartment comprised at least in part of gas permeable material, each culture compartment having a gas permeable, liquid impermeable planar shelf for cells to reside upon connected to an opposing surface, a shared fluid pathway connecting said culture compartments, a gas space in contact with at least a portion of each gas permeable, liquid impermeable shelf, each gas permeable, liquid impermeable shelf in contact with a culture compartment support, andplacing said apparatus within a cell culture incubator that includes ambient gas suitable for cell culture, said apparatus oriented in a culture position within said cell culture incubator such that said culture compartments reside one above the other, at least a portion of said ambient gas enters each said culture compartment by way of its said shelf, at least a portion of said cells are located upon said shelves, said culture compartments are full of media, and said shelves are horizontal. 40. A method of culturing animal cells, the method comprising: a first step of adding animal cells and media into a gas permeable multi-shelf apparatus comprising two or more culture spaces, each having a gas permeable, liquid impermeable shelf for cells to reside upon and an opposing surface, a portion of each said gas permeable, liquid impermeable shelf in contact with a culture compartment support that exposes at least a portion of each said gas permeable, liquid impermeable shelf to ambient gas and maintains said gas permeable, liquid impermeable shelf in a flat position when said gas permeable, liquid impermeable shelf is oriented in a horizontal position with media residing upon it, and a manifold that creates a fluid pathway between said culture compartments; anda second step consisting essentially of placing said gas permeable multi-shelf apparatus in a cell culture incubator wherein said apparatus is in a position such that said culture compartments reside one above the other, each said gas permeable, liquid impermeable shelf is in a horizontal position, animal cells reside upon at least a portion of each gas permeable, liquid impermeable shelf and said culture compartments include media. 41. The method of claim 40 wherein said cell culture incubator includes carbon dioxide. 42. The method of claim 40 wherein said cell culture incubator is set to maintain temperature at thirty seven degrees Celsius. 43. The method of claim 40 wherein the ratio of the ratio of the volume of media within said apparatus to the surface area of said gas permeable, liquid impermeable shelves is in a range of 0.2 ml/cm2 to 0.4 ml/cm2. 44. The method of claim 40 wherein each said gas permeable, liquid impermeable shelf for cells to reside upon is parallel to its opposing surface. 45. A method of culturing cell comprising: incubating cells in the presence of ambient gas suitable for cell culture, said cells residing in a gas permeable multi-shelf cell culture apparatus wherein said cells reside upon more than one shelf, each shelf comprised of gas permeable, liquid impermeable material, each shelf open to only one shared fluid pathway, said shared fluid pathway is located within said apparatus, a portion of the bottom of each shelf is in contact with a shelf support that maintains the shelf in a horizontal position, said ambient gas is in contact with the bottom of each said shelf, each said shelf has an opposing wall connected to it and residing above it, and said apparatus contains media in a static state. 46. A method of culturing cells, the method comprising: adding cells and media into a gas permeable multi-shelf apparatus comprising more than one culture compartment, each having a gas permeable, liquid impermeable surface and an opposing surface, a gas space separating the culture compartments and the gas space in communication with said gas permeable, liquid impermeable surface of said culture compartments, a gas space housing enclosing said gas space, said gas space housing including a gas access cover and an opening and a manifold to connect said culture compartments, andincubating said apparatus in the presence of ambient gas suitable for cell culture wherein said culture compartments are oriented one above the other, and including a step comprised of opening said gas access cover when incubating said apparatus in the presence of ambient gas suitable for cell culture and closing said gas access cover when not incubating said apparatus in the presence of ambient gas suitable for cell culture. 47. A method of culturing cells, the method comprising: adding cells and media into a gas permeable multi-shelf apparatus comprising more than one culture compartment, each having a gas permeable, liquid impermeable surface and an opposing surface, a gas space separating the culture compartments and the gas space in communication with said gas permeable, liquid impermeable surface of said culture compartments, a manifold to connect said culture compartments, a gas space housing including a gas exchange control rim extending from said gas space housing,incubating said apparatus in the presence of ambient gas suitable for cell culture wherein said culture compartments are oriented one above the other, and comprising a step of placing said apparatus in a position in which said gas exchange control rim is flush to a surface when not incubating said apparatus in the presence of ambient gas suitable for animal cell culture. 48. A method of culturing cells, the method comprising: adding cells and media into a gas permeable multi-shelf apparatus comprising more than one culture compartment, each having a gas permeable, liquid impermeable surface and an opposing surface, said culture compartments being connected by a manifold, a gas space separating the cell culture compartments and the gas space in communication with said gas permeable, liquid impermeable surface of said culture compartments, a gas space housing enclosing said gas space, said gas space housing including a gas access cover and an opening, andincubating said apparatus in the presence of ambient gas suitable for cell culture, wherein said culture compartments are oriented one above the other and wherein said ambient gas is not forced to flow into said gas space but instead moves into contact with said gas permeable, liquid impermeable surface by passive gas exchange and comprising a step of opening said gas access cover when incubating said apparatus in the presence of ambient gas suitable for cell culture and closing said gas access cover when not incubating said apparatus in the presence of ambient gas suitable for animal cell culture. 49. A method of culturing cells, the method comprising: adding cells and media into a gas permeable multi-shelf apparatus comprising more than one culture compartment oriented one above the other, each having a gas permeable, liquid impermeable surface and an opposing surface, said culture compartments being connected by a manifold, a gas space separating the cell culture compartments and the gas space in communication with said gas permeable, liquid impermeable surface of said culture compartments, a gas exchange control rim extending from a gas space housing, andincubating said apparatus in the presence of ambient gas suitable for cell culture wherein said culture compartments are oriented one above the other and wherein said ambient gas is not forced to flow into said gas space but instead moves into contact with said gas permeable, liquid impermeable surface by passive gas exchange and comprising a step of placing said apparatus in a position such that said gas exchange control rim is flush to a surface when not incubating said apparatus in the presence of ambient gas suitable for cell animal culture.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (73)
Bacehowski David V. (Wildwood IL) Breillatt ; Jr. Julian P. (Mundelein IL) Kolanko William (Grayslake IL) Smith Sidney T. (Lake Forest IL), Adherent cell culture flask.
Spielmann Richard (Rue d\Herinnes 43 B-7850 Enghien BEX), Apparatus having a rotatable stack of parallel trays with culturing surfaces on opposite sides for liquid/gas exchange.
Toner, Mehmet; Tilles, Arno W.; Balis, Ulysses J.; Yarmush, Martin L.; Cosman, Maury D.; Dimilla, Paul A., Cell culture systems and methods for organ assist devices.
DiMilla, Paul A.; Cosman, Maury D.; Halych, Rachel; Romito, Lisa; Gemmeti, Chris; Odlum, Kevin, Chamber with adjustable volume for cell culture and organ assist.
Butz David (Westford ME MA) Lyman George (Cape Porpoise ME) Root David (Lexington ME) Mathus Gregory (Concord MA), Culture device having a detachable cell or tissue growth surface.
Anderson Charles Daniel ; Dodd Charlie W., Culture vessel for growing or culturing cells, cellular aggregates, tissues and organoids and methods for using the same.
Schwarz Ray P. (League City TX) Wolf David A. (Houston TX) Trinh Tinh T. (Houston TX), Horizontally rotated cell culture system with a coaxial tubular oxygenator.
Pattillo Martha C. (Evanston IL) Bacehowski David V. (Wildwood IL) Bilstad Arnold C. (Deerfield IL) Cullis Herbert M. (Silver Spring MD) Dennehey T. Michael (Arlington Heights IL) Yang James W. (Scha, Method for metering nutrient media to cell culture containers.
Smith Sidney T. ; Bacehowski David V. ; Kolanko William ; Rosenbaum Larry ; Smith Stephen L. ; Bender James G. ; Woo Lecon ; Ling Michael T. K., Multilayer gas-permeable container for the culture of adherent and non-adherent cells.
Smith Sidney T. ; Bacehowski David V. ; Kolanko William ; Rosenbaum Larry ; Smith Stephen L. ; Bender James G. ; Woo Lecon ; Ling Michael T. K., Multilayer gas-permeable container for the culture of adherent and non-adherent cells.
Fuller, Jess Paul; Pegg, David; Bird, Robert McLean; Clifford, Timothy Burgess; Clayson, Tony, Preparation of porous silicone rubber for growing cells or living tissue.
Young Michael W. (Kennebunkport ME) Founds Henry W. (Scarborough ME) Zuk ; Jr. Peter (Harvard MA), Sparger and apparatus for and method of growing cells.
Pattillo Martha C. (Evanston IL) Bacehowski David V. (Wildwood IL) Bilstad Arnold C. (Deerfield IL) Cullis Herbert M. (Silver Spring MD) Dennehey T. Michael (Arlington Heights IL) Yang James W. (Scha, System for metering nutrient media to cell culture containers and method.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.