Cartridge containing reagent, microfluidic device including the cartridge, method of manufacturing the microfluidic device, and biochemical analysis method using the microfluidic device
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A microfluidic device including a platform and a cartridge is disclosed. The platform includes a chamber containing a fluid. The reagent cartridge is mounted to the platform. and contains a solid reagent for detecting material contained in the fluid.
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1. A microfluidic device comprising: a platform including a chamber containing a fluid; anda reagent cartridge mounted to the platform, the reagent cartridge comprising a closed first end, a closed second end, a sidewall connecting the first end and the second end, an opening formed in the sidewall,
1. A microfluidic device comprising: a platform including a chamber containing a fluid; anda reagent cartridge mounted to the platform, the reagent cartridge comprising a closed first end, a closed second end, a sidewall connecting the first end and the second end, an opening formed in the sidewall, and a well containing a solid reagent for detecting a material contained in the fluid,wherein the platform includes at least one detection chamber in which the reagent cartridge is mounted. 2. The microfluidic device of claim 1, wherein the solid reagent is a lyophilized solid reagent. 3. The microfluidic device of claim 1, wherein the microfluidic device comprises at least two reagent cartridges each containing the same or different lyophilized reagents from the other. 4. The microfluidic device of claim 1, wherein the reagent cartridge comprises a plurality of reagent wells each containing a different reagent. 5. The microfluidic device of claim 1, wherein at least part of the detection chamber is made of a transparent material and the at least part of the detection chamber is the part not housing the reagent cartridge. 6. The microfluidic device of claim 5, wherein the reagent cartridge is mounted in the detection chamber in such a way that the opening of the reagent cartridge faces the fluid flowing into the detection chamber. 7. The microfluidic device of claim 1, wherein the platform comprises: a sample chamber to accommodate the sample;a diluent chamber to accommodate a diluent; anda valve for controlling the flow of the fluid disposed at at least one point between said chambers. 8. The microfluidic device of claim 7, wherein the valve is controlled according to pressure of the fluid. 9. The microfluidic device of claim 8, wherein the pressure is generated when the microfluidic device rotates. 10. The microfluidic device of claim 7, wherein the valve is formed of a valve forming material that opens by electromagnetic radiation energy. 11. The microfluidic device of claim 10, wherein the valve forming material is selected from a phase transition material and a thermoplastic resin, wherein the phase of the phase transition material or the thermoplastic resin changes by electromagnetic radiation energy. 12. The microfluidic device of claim 10, wherein the valve forming material comprises micro heat-dissipating particles which are dispersed in a phase transition material, absorb the electromagnetic radiation energy, and dissipate the energy. 13. The microfluidic device of claim 7, further comprising a container coupled to the platform and providing the diluent to the diluent chamber. 14. The microfluidic device of claim 1, wherein the solid reagent comprises at least one reagent selected from the group consisting of reagents for detecting serum, aspartate aminotransferase (AST), albumin (ALB), alkaline phosphatase (ALP), alanine aminotransferase (ALT), amylase (AMY), urea nitrogen (BUN), calcium (Ca++), total cholesterol (CHOL), creatine kinase (CK), chloride (Cl−), creatinine (CREA), direct bilirubin (D-BIL), gamma glutamyl transferase (GGT), glucose (GLU), high-density lipoprotein cholesterol (HDL), potassium (K+), lactate dehydrogenase (LDH), low-density lipoprotein cholesterol (LDL), magnesium (Mg), phosphorus (PHOS), sodium (Na+), total carbon dioxide (TCO2), total bilirubin (T-BIL), triglycerides (TRIG), uric acid (UA), and total protein (TP). 15. The microfluidic device of claim 14, wherein the solid reagent comprises an additive. 16. The microfluidic device of claim 15, wherein the additive is a filler that comprises at least one material selected from the group consisting of bovine serum albumin (BSA), polyethylene glycol (PEG), dextran, mannitol, polyalcohol, myo-inositol, an citric acid, ethylene diamine tetra acetic acid disodium salt (EDTA2Na), and polyoxyethylene glycol dodecyl ether (BRIJ-35). 17. The microfluidic device of claim 15, wherein the additive is a surfactant that comprises at least one material selected from the group consisting of polyoxyethylene, lauryl ether, octoxynol, polyethylene alkyl alcohol, nonylphenol polyethylene glycol ether; ethylene oxide, ethoxylated tridecyl alcohol, polyoxyethylene nonylphenyl ether phosphate sodium salt, and sodium dodecyl sulfate. 18. The microfluidic device of claim 1, wherein at least a portion of a shape of the solid reagent is complementary to at least a portion of an inner configuration of the well of the reagent cartridge. 19. The microfluidic device of claim 7, wherein the detection chamber comprises a structure preventing the reagent cartridge from freely moving within the detection chamber. 20. The microfluidic device of claim 1, wherein the well of the reagent cartridge comprises a structure increasing retention of the solid reagent in the reagent cartridge. 21. A microfluidic device comprising: a platform comprising a chamber and channels;a reagent cartridge housed in the chamber, the reagent cartridge comprising a closed first end, a closed second end, a sidewall connecting the first end and the second end, and an opening formed in the sidewall to form a well; anda soluble solid reagent accommodated in the well of the reagent cartridge,wherein the reagent cartridge is mounted within the chamber and wherein the well of the reagent cartridge is in fluid communication with the chamber. 22. The microfluidic device according to claim 21, wherein the reagent cartridge is fitted into the chamber. 23. The microfluidic device according to claim 21, wherein the microfluidic device comprises a first chamber housing a first reagent cartridge and a second chamber housing a second reagent cartridge; wherein the first reagent cartridge contains a first reagent;wherein the second reagent cartridge contains a second reagent; andthe first reagent and the second reagent are the same or different. 24. The microfluidic device according to claim 23, wherein the first reagent and the second reagent are different from each other; and wherein the first chamber receives fluid which contacts the first reagent contained in the first reagent cartridge to form a first reaction mixture and the second chamber receives the first reaction mixture which contacts the second reagent contained in the second reagent cartridge to form a second reaction mixture. 25. The microfluidic device according to claim 21, wherein the reagent cartridge comprises at least two wells each accommodating a reagent. 26. The microfluidic device according to claim 21, wherein the well of the reagent cartridge comprises a structure to retain the reagent accommodated in the reagent cartridge. 27. The microfluidic device according to claim 26, wherein the structure is at least one protrusion formed inside the well. 28. The microfluidic device according to claim 21, wherein the chamber has an indentation retaining the reagent cartridge in the chamber. 29. The microfluidic device according to claim 21, wherein the chamber comprises a protrusion to hold the reagent cartridge in the chamber. 30. A cartridge adapted to be installed in a microfluidic device, the cartridge comprising: a body including a first end, a second end, a sidewall connected to the first end and to the second end, and an opening formed in the sidewall to form a well in the body; anda solid reagent contained in the well in a unit usage amount,wherein the body is mounted within a chamber of the microfluidic device and wherein the well is in fluid communication with the chamber. 31. The cartridge according to claim 30, wherein a shape of at least one portion of the reagent is complementary to a portion of an internal shape of well. 32. The cartridge of claim 30, wherein the body comprises at least two wells each containing a solid reagent. 33. The cartridge of claim 30, further comprising a structure provided in the well to retain the solid reagent accommodated therein. 34. The cartridge of claim 33, wherein the structure is at least one protrusion formed inside the well. 35. A cartridge suitable for a microfluidic device, comprising: a body including a closed first end, a closed second end, a closed wall connecting the first and second ends, an opening formed in the wall, and a well accessible through the opening; anda solid reagent contained in the well,wherein the body is mounted within a chamber of the microfluidic device and wherein the well is in fluid communication with the chamber.
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