Rapid isolation of osteoinductive protein mixtures from mammalian bone tissue
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C07K-014/51
C07K-014/145
C07K-001/14
C07K-001/34
A61K-038/17
출원번호
US-0582476
(2009-10-20)
등록번호
US-8829166
(2014-09-09)
발명자
/ 주소
Thorne, Kevin
Akella, Rama
출원인 / 주소
Zimmer Orthobiologics, Inc.
대리인 / 주소
Schwegman Lundberg & Woessner, P.A.
인용정보
피인용 횟수 :
0인용 특허 :
215
초록▼
A method for purifying bone-derived osteoinductive proteins including a demineralization process, a protein extraction process, a high molecular weight ultrafiltration process, a low molecular weight ultrafiltration process, and a recover process. The high and low ultrafiltration processes preferabl
A method for purifying bone-derived osteoinductive proteins including a demineralization process, a protein extraction process, a high molecular weight ultrafiltration process, a low molecular weight ultrafiltration process, and a recover process. The high and low ultrafiltration processes preferably select proteins having a nominal molecular weight between approximately 8 kilodaltons and approximately 100 kilodaltons. Processes of the present invention may be used to recover osteoinductive proteins from bone demineralization waste streams.
대표청구항▼
1. A method of producing an osteoinductive composition, comprising: contacting bone tissue with a demineralization medium consisting essentially of an acidic solution and optionally a bone mineral solubility enhancer, a defoaming agent or both to provide demineralized bone tissue and a mineralized s
1. A method of producing an osteoinductive composition, comprising: contacting bone tissue with a demineralization medium consisting essentially of an acidic solution and optionally a bone mineral solubility enhancer, a defoaming agent or both to provide demineralized bone tissue and a mineralized supernatant;separating the mineralized supernatant from the demineralized bone tissue;recovering osteoinductive proteins from the mineralized supernatant; andcombining the osteoinductive proteins with a carrier. 2. The method of claim 1, wherein the carrier comprises a solid. 3. The method of claim 2, wherein the solid carrier is selected from the group consisting of collagen, a bone derivative, hydroxyapatite or a composite device. 4. The method of claim 2, wherein the solid carrier comprises a bone derivative selected from the group consisting of bone chips or bone matrix. 5. The method of claim 2, wherein the solid carrier comprises bone matrix. 6. The method of claim 5, wherein the bone matrix comprises demineralized and devitalized bone matrix (DVBM). 7. The method of claim 1, wherein the carrier comprises a liquid. 8. The method of claim 7, wherein the liquid carrier is selected from the group consisting of saline, a blood-derivative or bone marrow aspirate. 9. The method of claim 7, wherein the liquid carrier comprises a blood derivative selected from the group consisting of whole blood, serum, plasma or platelet rich plasma (PRP). 10. The method of claim 2, wherein the liquid carrier comprises serum. 11. The method of claim 2, wherein the liquid carrier comprises saline. 12. The method of claim 1, wherein the carrier comprises a slurry. 13. The method of claim 1, further comprising storing the osteoinductive proteins as a solution or a solid prior to or after combining with the carrier. 14. The method of claim 13, wherein the osteoinductive proteins are stored sterile. 15. The method of claim 13, wherein the osteoinductive proteins are stored under vacuum or an inert gas atmosphere. 16. The method of claim 15, wherein the inert gas is selected from the group consisting of nitrogen, hydrogen, helium, argon or mixtures thereof. 17. The method of claim 13, wherein the osteoinductive proteins are stored as a solid. 18. The method of claim 17, wherein the osteoinductive proteins are lyophilized. 19. The method of claim 1, further comprising removing at least some mineral from the mineralized supernatant prior to or while recovering osteoinductive proteins. 20. The method of claim 19, wherein removing comprises performing tangential flow filtration of the mineralized supernatant. 21. The method of claim 19, wherein removing comprises contacting the mineralized supernatant with mineral precipitation agent. 22. The method of claim 1, wherein the solubility enhancer is a salt or solvent. 23. The method of claim 22, wherein the salt is CaCl2 or ethylenediamine tetraacetic acid (EDTA). 24. The method of claim 1, wherein the defoaming agent is an alcohol, hydrocarbon, fatty acid, fatty acid salt of a multivalent cation, ester, amide or silicone oil or silica. 25. The method of claim 24, wherein the alcohol is octyl alcohol. 26. The method of claim 24 wherein the hydrocarbon is mineral oil, white oil or paraffin. 27. The method of claim 24, wherein the ester is a fat, wax or phosphoric ester. 28. The method of claim 24, wherein the amide is an amide wax. 29. The method of claim 1, wherein the defoaming agent is an alcohol.
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Catsimpoolas Nicholas (Newton Centre MA) Goldsmith Harry S. (Boston MA), Angiogenic factor methods of extraction and method for producing angiogenesis.
Seliktar Dror ; Dunkelman Noushin ; Peterson Alvin Edward ; Schreiber Ronda Elizabeth ; Willoughby Jane ; Naughton Gail K., Application of shear flow stress to chondrocytes or chondrocyte stem cells to produce cartilage.
Sittinger Michael,DEX ; Schultz Olaf,DEX ; Burmester Gerd R.,DEX ; Haupl Thomas E. M.,DEX, Artificial tissues, methods for the production and the use thereof.
Celeste Anthony J. ; Wozney John M. ; Rosen Vicki A. ; Wolfman Neil M. ; Thomsen Gerald H. ; Melton Douglas A., BMP-12 and BMP-13 proteins and DNA encoding them.
Berguer Ramon (West Bloomfield MI) Farnan Robert C. (Tucson AZ) Colone William M. (Phoenix AZ) Kowligi Rajagopal R. (Tempe AZ) Della Corna Linda V. (Glendale AZ) Sinnott Joseph B. (Mesa AZ), Blood vessel patch.
Brendel Klaus (Tucson AZ) Duhamel Raymond C. (Tucson AZ), Body implants of extracellular matrix and means and methods of making and using such implants.
Adams Steven W. (Sunnyvale CA) Armstrong Rosa (Palo Alto CA) Rosen David (San Jose CA), Bone growth factors and inhibitors of bone resorption for promoting bone formation.
Caplan Arnold (1300 Oakridge Dr. Cleveland Heights OH 44121) Syftestad Glenn T. (3660 Warrensville Center Rd. - #101 Shaker Heights OH 44122), Bone protein purification process.
Joseph C. Glorioso ; Christopher H. Evans ; Paul D. Robbins ; Richard Kane, Cartilage alterations by administering to joints chondrocytes comprising a heterologous polynucleotide.
Chu George H. (Sunnyvale CA) Ogawa Yasushi (Pacifica CA) McPherson John M. (Hopkinton MA) Ksander George (Redwood City CA) Pratt Bruce (Union City CA) Hendricks Diana (Brea CA) McMullin Hugh (San Bru, Collagen wound healing matrices and process for their production.
Bittmann, Pedro; Atkinson, Brent; Benedict, James J.; Ranieri, John; Whitney, Marsha L.; Chickering, Donald, Composition and device for in vivo cartilage repair.
Sporn Michael B. ; Roberts Anita B., Compositions of transforming growth factor .beta.(TGF-.beta.) which promotes wound healing and methods for their use.
Wozney John M. (Hudson MA) Wang Elizabeth A. (Carlisle MA) Rosen Vicki A. (Brookline MA) Celeste Anthony J. (Hudson MA), DNA sequences encoding BMP-6 proteins.
Laurencin Cato T. (Somerville MA) Lucas Paul A. (Macon GA) Syftestad Glenn T. (Sacramento CA) Domb Abraham (Baltimore MD) Glowacki Julianne (Jamaica Plan MA) Langer Robert S. (Newton MA), Delivery system for controlled release of bioactive factors.
Laurencin Cato T. (Somerville MA) Lucas Paul A. (Macon GA) Syftestad Glenn T. (Sacramento CA) Domb Abraham (Baltimore MD) Glowacki Julie (Jamaica Plan MA) Langer Robert S. (Newton MA), Delivery system for controlled release of bioactive factors.
Parsons John R. (Perth Amboy NJ) Lee Casey K. (Short Hills NJ) Langrana Noshir A. (Robbinsville NJ) Clemow Alastair J. (North Smithfield RI) Chen Elizabeth H. (Sharon MA) Hawkins Monica V. (Parsippan, Functional and biocompatible intervertebral disc spacer containing elastomeric material of varying hardness.
Noh Moon Jong,KRX ; Kang Kyoung Ae,KRX ; Lee Kwan Hee,KRX, Generating cartilage in a mammal using fibroblasts transfected with a vector encoding TGF-.beta.-1.
Rhee Woonza M. (3845 LaDonna Ave. Palo Alto CA 94306) Berg Richard A. (660 S. Springer Rd. Los Altos CA 94024), Glycosaminoglycan-synthetic polymer conjugates.
Beam,Heather Ann; Bradbury,Thomas J.; Chesmel,Kathleen D.; Gaylo,Christopher M.; Litwak,Alfred Anthony; Liu,Qing; Materna,Peter Albert; Monkhouse,Donald; Patterson,Jennifer; Pryor,Timothy J.; Saini,S, Method and apparatus for engineered regenerative biostructures such as hydroxyapatite substrates for bone healing applications.
Ogawa Yasushi (Pacifica CA) Schmidt David K. (Santa Cruz CA) Armstrong Rosa (Palo Alto CA) Nathan Ranga (Newark CA) Thompson Andrea Y. (Mountain View CA) Seyedin Saeid M. (Saratoga CA), Method for inducing bone growth.
Maubois,Jean Louis; Fauguant,Jacques; Jouan,Pierre; Bourtourault,Michel, Method for obtaining a TGF-beta enriched protein fraction in activated form, protein fraction and therapeutic applications.
Piez Karl A. (Menlo Park CA) Pharriss Bruce B. (Palo Alto CA) Chu George H. (Sunnyvale CA) Smestad Thomas L. (Palo Alto CA) Hendricks Diana (Palo Alto CA), Method of making molds with xenogeneic collagen/mineral preparations for bone repair.
Burgess, Willson H.; Greisler, Howard P.; Drohan, William N.; Maciag, Thomas; MacPhee, Martin J., Method of preparing a tissue sealant-treated biomedical material.
Campbell Todd D. (Corona CA) Nashef Aws S. (Costa Mesa CA), Method of producing prostheses for replacement of articular cartilage and prostheses so produced.
Sehl, Louis C.; Trollsas, Olof Mikael; Wallace, Donald G.; Toman, David; DeLustro, Frank A.; Schroeder, Jacqueline A.; Chu, George H., Methods for tissue repair using adhesive materials.
Wallace Donald G. (Menlo Park CA) Smestad Thomas L. (Palo Alto CA) McPherson John M. (Sunnyvale CA) Piez Karl A. (Menlo Park CA) Seyedin Saeid (Sunnyvale CA) Armstrong Rosa (Palo Alto CA), Methods of bone repair using collagen.
Celeste Anthony J. (Hudson MA) Wozney John M. (Hudson MA) Rosen Vicki A. (Brookline MA) Wolfman Neil M. (Dover MA) Thomsen Gerald H. (Port Jefferson NY) Melton Douglas A. (Lexington MA), Methods of inducting formation of tendon and/or ligament tissue comprising administering BMP-12, BMP-13, and/or MP-52.
Cohen Charles M. ; Charette Marc F. ; Kuberasampath Thangavel ; Rueger David C. ; Oppermann Hermann ; Pang Roy H. L., Morphogen treatment of gastrointestinal ulcers.
Poser James W. (12180 W. 18th Dr. Lakewood CO 80215) Benedict James J. (13206 W. 33rd Pl. Golden CO 80401), Osteoinductive protein mixtures and purification processes.
Seyedin Saeid (Sunnyvale CA) Thomas Thomas (Concord CA) Bentz Hanne (Palo Alto CA) Ellingsworth Larry (San Jose CA) Armstrong Rosa (Palo Alto CA), Polypeptide cartilage-inducing factors found in bone.
Seyedin Saeid (Sunnyvale CA) Thomas Thomas (Davis CA) Bentz Hanne (Palo Alto CA) Ellingsworth Larry (San Jose CA) Armstrong Rosa (Palo Alto CA), Polypeptide cartilage-inducing factors found in bone.
Seyedin Saeid (Sunnyvale CA) Thomas Thomas (Davis CA) Bentz Hanne (Palo Alto CA) Ellingsworth Larry (San Jose CA) Armstrong Rosa (Palo Alto CA), Polypeptide cartilage-inducing factors found in bone.
Seyedin Saeid (Sunnyvale CA) Thomas Thomas (Concord CA) Bentz Hanne (Palo Alto CA) Ellingsworth Larry (San Jose CA) Armstrong Rosa (Palo Alto CA), Polypeptide cartilage-inducing factors found in bone used in tissue proliferation.
Hyon Suong-Hyu (Tamatsukurimotomachi JPX) Ikada Yoshito (Tamatsukurimotomachi JPX), Porous and transparent poly(vinyl alcohol) gel and method of manufacturing the same.
Daniels John R. (Menlo Park CA) Knapp Terry R. (Santa Clara CA), Process for augmenting connective mammalian tissue with in situ polymerizable native collagen solution.
Kussendrager Klaas Daniel,NLX ; Kivits Marinus Gerardus Cornelis,NLX ; Lemmen Hubert Karel,NLX ; van Kessel Theodorus Johannes Antonia Maria,NLX, Process for recovering growth factors, or a composition containing one or more growth factors, from milk or a milk derivative.
Caplan Arnold I. (1300 Oakridge Dr. Cleveland Heights OH 44121) Syftestad Glenn T. (3660 Warrensville Center Rd. #101 Shaker Heights OH 44122), Process of adapting soluble bone protein for use in stimulating osteoinduction.
Caplan Arnold (1300 Oakridge Dr. Cleveland Heights OH 44121) Syftestad Glenn T. (3660 Warrensville Center Rd. #101 Shaker Heights OH 44122), Process of and material for stimulating growth of cartilage and bony tissue at anatomical sites.
Thiele Heinrich (Neue Universitaet Kiel DEX) Thiele Erhard (Reventlow-Allee 9 23 Kiel DEX), Process of degrading and regenerating bone and tooth material and products.
Heggeness Michael H. (3301 Drummond Houston TX 77025) Doherty Brian J. (350 Sharon Park Dr. Apt. E 39 Menlo Park CA 94025), Prosthetic intervertebral devices.
Moore-Smith, Debra; O'Leary, Robert K.; Wilson, Anne, Pulsatile acidification wave demineralization process for producing osteoinductive bone; and osteoinductive bone produced thereby.
MacPhee Martin James ; Drohan William Nash ; Woolverton Christoper J., Supplemented and unsupplemented tissue sealants, methods of their production and use.
Bret A. Ferree, Treating degenerative disc disease through transplantion of extracellular nucleus pulposus matrix and autograft nucleus pulposus cells.
Yuan-Di C. Halvorsen ; William O. Wilkison ; Jeffrey Martin Gimble, Use of adipose tissue-derived stromal cells for chondrocyte differentiation and cartilage repair.
Bentz Hanne (Newark CA) Thompson Andrea Y. (Mountain View CA) Armstrong Rosa (Palo Alto CA) Rosen David M. (San Jose CA), Use of bone morphogenetic protein in synergistic combination with TGF-b
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Bolander Mark E. (Washington DC) Balian Gary (Charlottesville VA), Use of demineralized bone matrix in the repair of segmental defects.
Martin, Ivan; Freed, Lisa E.; Langer, Robert; Vunjak-Novakovic, Gordana, Use of fibroblast growth factor 2 for expansion of chondrocytes and tissue engineering.
Yuan Hansen A. (5066 Pine Valley Dr. Fayetteville NY 13066) Lin Chih-I (14292 Spring Vista La. Chino Hills CA 91709), Vertebral auxiliary fixation device having holding capability.
Piez Karl A. (Menlo Park CA) Pharriss Bruce B. (Palo Alto CA) Chu George H. (Sunnyvale CA) Smestad Thomas L. (Palo Alto CA) Hendricks Diana (Palo Alto CA), Xenogeneic collagen/mineral preparations in bone repair.
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