[미국특허]
Isolated polynucleotides and polypeptides and methods of using same for increasing plant utility
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12N-015/82
C12N-015/87
출원번호
US-0992902
(2009-05-21)
등록번호
US-8847008
(2014-09-30)
국제출원번호
PCT/IL2009/000508
(2009-05-21)
§371/§102 date
20101220
(20101220)
국제공개번호
WO2009/141824
(2009-11-26)
발명자
/ 주소
Emmanuel, Eyal
Diber, Alex
Gold, Evgenia
Nevo, Inbar
Vinocur, Basia Judith
Ayal, Sharon
Ronen, Gil
Herschkovitz, Yoav
Gang, Michael
Dimet, Dotan
Idan, Anat
출원인 / 주소
Evogene Ltd.
인용정보
피인용 횟수 :
2인용 특허 :
68
초록▼
Provided are isolated polypeptides comprising the amino acid sequence at least 80% homologous to SEQ ID NO:68, 51-66, 69-100, 379-656, 707-715, 720-723, 742-754, 764-771 or 772 with the proviso that the amino acid sequence is not as set forth by SEQ ID NO: 765 or 771, isolated polynucleotides compri
Provided are isolated polypeptides comprising the amino acid sequence at least 80% homologous to SEQ ID NO:68, 51-66, 69-100, 379-656, 707-715, 720-723, 742-754, 764-771 or 772 with the proviso that the amino acid sequence is not as set forth by SEQ ID NO: 765 or 771, isolated polynucleotides comprising the nucleic acid sequence at least 80% identical to SEQ ID NOs:18, 1-16, 19-50, 101-378, 657-672, 674-706, 716-719, 724-741 and 755-763 with the proviso that the nucleic acid sequence is not as set forth by SEQ ID NO:756 or 762, and isolated polynucleotides selected from the group consisting of SEQ ID NOs:779-792 and methods of using same for increasing oil content, yield, growth rate, biomass, vigor, abiotic stress tolerance and/or nitrogen use efficiency of a plant.
대표청구항▼
1. A method of increasing oil in a plant, comprising: (a) providing a plant transformed with a nucleic acid construct which comprises the nucleic acid sequence set forth in SEQ ID NOs:18 or 674, wherein said nucleic acid sequence encodes the polypeptide set forth in SEQ ID NO:68;(b) expressing the p
1. A method of increasing oil in a plant, comprising: (a) providing a plant transformed with a nucleic acid construct which comprises the nucleic acid sequence set forth in SEQ ID NOs:18 or 674, wherein said nucleic acid sequence encodes the polypeptide set forth in SEQ ID NO:68;(b) expressing the polypeptide of SEQ ID NO: 68 in said transformed plant, and(c) extracting the oil from the transformed plant, and wherein said transformed plant has increased oil as compared to a wild-type plant of the same species. 2. The method of claim 1, wherein said nucleic acid sequence is operably linked to a heterologous promoter for directing transcription of said nucleic acid sequence in a plant cell of said transformed plant. 3. The method of claim 2, wherein said promoter comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:779, 780, 781, 782, 783, 784, 785, 786, 787, 788, 789, 790, 791 and 792. 4. A method of increasing oil content, yield, growth rate, biomass, or tolerance salinity stress of a plant as compared to a wild type plant of the same species which is grown under the same growth conditions, comprising: (a) transforming plants with an exogenous polynucleotide comprising a nucleic acid sequence encoding the polypeptide set forth in SEQ ID NO: 68; and(b) selecting a transformed plant from step (a) that expresses the tide of SEQ ID NO: 68 and exhibits increased oil content, increased yield, increased growth rate, increased biomass, or increased tolerance to salinity stress as compared to a wild type plant of the same species which is grown under the same growth conditions. 5. The method of claim 4, wherein said nucleic acid sequence encoding the polypeptide of SEQ ID NO: 68 set forth in SEQ ID NO: 18 or SEQ ID NO: 674. 6. The method of claim 1, further comprising growing the transformed plant expressing said exogenous polynucleotide encoding said polypeptide of SEQ ID NO: 68 under the salinity stress condition. 7. A method of producing a crop plant comprising: (a) transforming parent plants with an exogenous polynucleotide encoding the polypeptide set forth in SEQ ID NO:68;(b) obtaining a population of transgenic crop plants from step (a); and(c) selecting a transformed crop plant from step (b) which expresses the polypeptide of SEQ ID NO:68 encoded by said exogenous polynucleotide, and exhibits increased oil content, increased yield, increased growth rate, increased biomass, or increased tolerance to salinity stress as compared to a wild type plant of the same species which is grown under the same growth conditions. 8. The method of claim 7, wherein the transformed crop plant of step (c) is selected for increased tolerance to salinity stress as compared to a wild type plant of the same species which is grown under the same growth conditions. 9. The method of claim 7, wherein the transformed crop plant of step (c) is selected for increased oil content as compared to a wild type plant of the same species which is grown under the same growth conditions. 10. The method of claim 7, wherein the transformed crop plant of step (c) is selected for increased biomass as compared to a wild type plant of the same species which is grown under the same growth conditions.
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