The present invention efficiently and cost-effectively extracts and collects cells from a tissue. The inventors have discovered that the tissue can be effectively fragmented and the resulting cells can be purified using a system or kit with multiple components. An advantage of the present invention
The present invention efficiently and cost-effectively extracts and collects cells from a tissue. The inventors have discovered that the tissue can be effectively fragmented and the resulting cells can be purified using a system or kit with multiple components. An advantage of the present invention is that tissue processing takes place in a closed system such that sterility can be maintained throughout the process, even if certain components are removed during processing, for example through the use of valves, clamps, and heat seals. Furthermore, any or all of the steps can be automated or manually accomplished, according to the specific needs of the application or the user.
대표청구항▼
1. A method of mincing a tissue sample positioned between a solid member and a cutting surface of a tissue mincing tool, the method comprising: pressing the tissue sample, via the solid member, into contact with and through the cutting surface of the tissue mincing tool, the tissue sample comprising
1. A method of mincing a tissue sample positioned between a solid member and a cutting surface of a tissue mincing tool, the method comprising: pressing the tissue sample, via the solid member, into contact with and through the cutting surface of the tissue mincing tool, the tissue sample comprising Wharton's Jelly stem cells, and the tissue mincing tool comprising: a compartment for the tissue sample; the solid member moveable within the compartment; the cutting surface at one end of the compartment; and a sterile, sealed container, wherein the cutting surface separates the compartment from the sterile, sealed container, such that the tissue sample that passes through the cutting surface is deposited as a viscous minced tissue within the sterile, sealed container. 2. A method of digesting a tissue sample, the method comprising: mincing the tissue sample according to the method of claim 1 and, optionally, transferring the minced tissue to a second sterile, sealed container; andinjecting an enzyme, whereby the enzyme digests the minced tissue. 3. The method of claim 2, wherein the enzyme is a collagenase. 4. A method of separating cells from a tissue sample, the method comprising: digesting the tissue sample according to the method of claim 2; andremoving undigested tissue. 5. The method of claim 4, further comprising diluting the tissue sample, wherein the undigested tissue is subsequently removed by filtering. 6. The method of claim 1, wherein the cutting surface is dimensioned to mince the tissue sample into fragments having an average cross-section no greater than four square millimeters. 7. The method of claim 1, wherein the cutting surface is dimensioned to mince the tissue sample into fragments, and the tissue mincing tool further comprises a second cutting surface for reducing an average cross-section of the fragments. 8. The method of claim 1, wherein the tissue mincing tool further comprises at least one mincer screen positioned in proximity to the cutting surface. 9. The method of claim 8, wherein the at least one mincer screen is moveable towards the tissue sample. 10. The method of claim 1, wherein the tissue mincing tool further comprises a suction cup for stabilizing the tissue mincing tool during operation thereof. 11. The method of claim 1, wherein the sterile, sealed container comprises at least one sealed access port permitting the sterile introduction of a fluid into the container. 12. The method of claim 1, wherein an interior surface of the compartment is threaded. 13. The method of claim 1, wherein the tissue mincing tool further comprises a gasket for sealing the compartment. 14. The method of claim 1, wherein a portion of the compartment near its end has a substantially constant cross-section. 15. The method of claim 14, wherein the solid member is shaped to mate with and to fill the portion of the compartment. 16. The method of claim 1, wherein the cutting surface is rotatable. 17. The method of claim 1, wherein the cutting surface is moveable towards the tissue sample. 18. The method of claim 1, wherein the tissue mincing tool further comprises a shaft crank for moving the cutting surface towards the tissue sample. 19. The method of claim 1, wherein the tissue sample is gelatinous. 20. The method of claim 1, wherein the tissue sample comprises umbilical cord tissue. 21. The method of claim 1, wherein the tissue mincing tool comprises a series of cutting surfaces at the one end of the compartment.
Okuda Morihiro,JPX ; Takagi Nobuo,JPX ; Fukuta Daisuke,JPX, Blood component-recovering apparatus and a method for recovering blood components using the same.
Shetty, Prathibha; Viswanathan, Chandra, Growth of neural precursor cells using umbilical cord blood serum and a process for the preparation for therapeutic purposes.
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