[특허]Caudal motor neuron derived from embryonic stem cells under conditions essentially free of any retinoid

[미국특허] Caudal motor neuron derived from embryonic stem cells under conditions essentially free of any retinoid 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	C12N-005/02 C12N-005/0793 C12N-005/074 C12N-005/0735 C12N-005/0797 A61K-035/30
출원번호	US-0653217 (2009-12-09)
등록번호	US-8969081 (2015-03-03)
발명자 / 주소	Wichterle, Hynek Jessell, Thomas M. Peljto, Mirza
출원인 / 주소	The Trustees of Columbia University in the City of New York
대리인 / 주소	White, John P.
인용정보	피인용 횟수 : 0 인용 특허 : 21

초록 ▼

Disclosed are methods for generating a neuron expressing Hoxc8 transcription factor or a caudal motor neuron comprising culturing an embryonic stem cell in a composition which is essentially free of retinoids and comprises an isotonic salt solution, so as to generate the neuron which expresses Hoxc8 transcription factor or the caudal motor neuron. Disclosed are also methods for generating a caudal brachial motor neuron, a thoracic motor neuron, or a lumbar motor neuron from an embryonic stem cell in a composition essentially free of retinoids and comprising ADFNK medium, an amount of FGF-2, or Gdf11 respectively. Disclosed are also methods of transplanting a motor neuron into a subject comprising generating the motor neuron and transplanting the motor neuron into the subject. Disclosed is also a population of motor neuron cells enriched for motor neuron cells expressing Foxp1 and expressing a gene associated with Spinal Muscular Atrophy (SMA) or Amyotrophic Lateral Sclerosis (ALS).

대표청구항 ▼

1. A method for producing a caudal brachial motor neuron from an embryonic stem cell, comprising a) culturing the embryonic stem cell in a composition essentially free of any retinoid and comprising Advanced Dulbecco's Modified Eagle's Medium/F12 and Neurobasal medium that has been supplemented with 10% Knockout-Serum Replacement (ADFNK medium) wherein the amount of the composition is effective to generate the caudal brachial motor neuron under conditions such that the caudal brachial motor neuron is produced, andb) recovering the caudal brachial motor neuron so produced. 2. The method of claim 1, wherein during culturing the embryonic stem cell is contacted with at least one neurotrophic factor. 3. The method of claim 1, wherein the embryonic stem cell is a murine embryonic stem cell. 4. The method of claim 1, wherein the embryonic stem cell is a human embryonic stem cell. 5. The method of claim 1, wherein the caudal brachial motor neuron is transfected so that it expresses enhanced green fluorescent protein (eGFP). 6. The method of claim 1, wherein the culturing is effected in vivo in a subject. 7. The method of claim 1, wherein the culturing is effected in vitro. 8. A method for producing a caudal motor neuron comprising a) culturing an embryonic stem cell in a serum-free neural induction composition which is essentially free of any retinoid and comprises an isotonic salt solution, wherein the amount of the composition is effective to generate the caudal motor neuron under condition such that the caudal motor neuron is produced, andb) recovering the caudal motor neuron so produced. 9. The method of claim 8, wherein during culturing the embryonic stem cell is contacted with at least one neurotrophic factor. 10. The method of claim 8, wherein the embryonic stem cell is a murine embryonic stem cell. 11. The method of claim 8, wherein the embryonic stem cell is a human embryonic stem cell. 12. The method of claim 8, wherein the caudal motor neuron is transfected so that it expresses enhanced green fluorescent protein (eGFP). 13. The method of claim 8, wherein the culturing is effected in vivo in a subject. 14. The method of claim 8, wherein the culturing is effected in vitro. 15. The method of claim 8, wherein composition further comprises an amount of Fibroblast Growth Factor-2 (FGF-2). 16. The method of claim 8, wherein composition further comprises an amount of Growth differentiation factor 11 (Gdf11). 17. The method of claim 8, wherein composition further comprises an amount of a Wnt factor. 18. The method of claim 8, wherein composition further comprises an amount of Fibroblast Growth Factor-2 (FGF-2), an amount of Growth differentiation factor 11 (Gdf11), and an amount of a Wnt factor.

이 특허에 인용된 특허 (21) 인용/피인용 타임라인 분석

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Johe Karl K., In vitro generation of differentiated neurons from cultures of mammalian multipotential CNS stem cells.
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Carpenter, Melissa K., Making neural cells for human therapy or drug screening from human embryonic stem cells.
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Porter, Jeffrey; Guicherit, Oivin M.; Rubin, Lee; Baxter, Anthony David; Boyd, Edward Andrew; Price, Stephen, Small organic molecule regulators of cell proliferation.
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Wilson Elaine Lynette ; Gabrilove Janice, Stimulation, production, culturing and transplantation of stem cells by fibroblast growth factors.
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Jessell, Thomas; Wichterle, Hynek; Wilson, Sara, Systems and methods for screening for modulators of neural differentiation.
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Weiss Samuel,CAX ; Reynolds Brent,CAX ; Hammang Joseph P. ; Baetge E. Edward, Use of multipotent neural stem cells and their progeny for the screening of drugs and other biological agents.
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Ingham Philip W.,GB2 ; McMahon Andrew P. ; Tabin Clifford J., Vertebrate embryonic pattern-inducing proteins, and uses related thereto.
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IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

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[미국특허] Caudal motor neuron derived from embryonic stem cells under conditions essentially free of any retinoid 원문보기