Enhancement of placental stem cell potency using modulatory RNA molecules
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A61K-048/00
A61K-035/50
A61K-031/7088
A61K-031/7105
A61K-031/713
C12N-005/073
C12N-005/0775
C12N-015/113
A61K-035/12
출원번호
US-0340589
(2011-12-29)
등록번호
US-8969315
(2015-03-03)
발명자
/ 주소
Abbot, Stewart
Karasiewicz-Mendez, Kathy E.
Voskinarian-Berse, Vanessa
Zhang, Xiaokui
출원인 / 주소
Anthrogenesis Corporation
대리인 / 주소
Jones Day
인용정보
피인용 횟수 :
2인용 특허 :
211
초록▼
Provided herein are methods of producing enhanced placental stem cells by modulatory RNA molecules. Also provided herein are methods of using enhanced placental stem cells, for example, to treat individuals having a disease, disorder or condition caused by, or relating to, an unwanted or harmful imm
Provided herein are methods of producing enhanced placental stem cells by modulatory RNA molecules. Also provided herein are methods of using enhanced placental stem cells, for example, to treat individuals having a disease, disorder or condition caused by, or relating to, an unwanted or harmful immune response. Further provided herein are compositions comprising said enhanced placental stem cells.
대표청구항▼
1. A method of treating an individual having or at risk of developing a disease, disorder or condition having an inflammatory component, comprising administering to the individual a therapeutically effective amount of enhanced placental stem cells, wherein said enhanced placental stem cells comprise
1. A method of treating an individual having or at risk of developing a disease, disorder or condition having an inflammatory component, comprising administering to the individual a therapeutically effective amount of enhanced placental stem cells, wherein said enhanced placental stem cells comprise or have been contacted with an effective amount of modulatory RNA molecules that decrease expression of one or more human nuclear receptors in said enhanced placental stem cells, resulting in suppression of soluble IL-23 protein produced by peripheral blood mononuclear cells (PBMCs) in the presence of said enhanced placental stem cells compared to placental stem cells not contacted with said modulatory RNA molecules,wherein said modulatory RNA molecules are small interfering RNAs (siRNAs), microRNA inhibitors (miR inhibitors), or micro RNA mimics (miR mimics),wherein said one or more human nuclear receptors is selected from the group consisting of: vitamin D receptor (VDR); nuclear receptor subfamily 4, group A, member 3 (NR4A3); nuclear receptor subfamily 0, group B, member 2 (NR0B2); nuclear receptor subfamily 1, group 1, member 2 (NR1I2); nuclear receptor subfamily 1, group H, member 3 (NR1H3); and deoxynucleotidyltransferase, terminal, interacting protein 1 (DNTTIP1), andwherein the therapeutically effective amount is an amount sufficient to cause a detectable improvement in one or more symptoms of said disease, disorder or condition. 2. The method of claim 1, wherein said modulatory RNA molecules are siRNAs. 3. The method of claim 1, wherein said modulatory RNA molecules are selected from a library. 4. The method of claim 3, wherein said library is a human nuclear receptor library, human phosphatase siRNA library, or an anti-miR library. 5. The method of claim 1, wherein said placental stem cells are CD10−, CD34−, CD105+, CD200+ placental stem cells. 6. The method of claim 1, wherein said placental stem cells express CD200 and do not express HLA-G, or express CD73, CD105, and CD200, or express CD200 and OCT-4, or express CD73 and CD105 and do not express HLA-G. 7. The method of claim 5, wherein said placental stem cells are additionally CD90+ and CD45−. 8. The method of claim 5, wherein said placental stem cells are additionally CD80− and CD86−.
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이 특허에 인용된 특허 (211)
Pittenger Mark F., Adipogenic differentiation of human mesenchymal stem cells.
Kuypers Franciscus A. ; Cole Robert B. ; Meyst Richard P. ; Gorton Lanny A. ; Wright James I., Apparatus and method for collecting blood from an umbilical cord.
Kuypers Franciscus A. ; Cole Robert B. ; Meyst Richard P. ; Gorton Lanny A. ; Wright James I., Apparatus and method for collecting blood from an umbilical cord.
Kuypers Franciscus A. ; Cole Robert B. ; Meyst Richard P. ; Gorton Lanny A. ; Wright James I., Apparatus and method for collecting blood from an umbilical cord.
Sanchez-Ramos, Juan; Song, Shijie; Janssen, William; Sanberg, Paul; Freeman, Thomas, Bone marrow cells as a source of neurons for brain and spinal cord repair.
Capecchi Mario R. (Salt Lake City UT) Thomas Kirk R. (Salt Lake City UT), Cells and non-human organisms containing predetermined genomic modifications and positive-negative selection methods and.
Capecchi Mario R. (Salt Lake City UT) Thomas Kirk R. (Salt Lake City UT), Cells and non-human organisms containing predetermined genomic modifications and positive-negative selection methods and.
Heidaran, Mohammad; Wang, Jia-Lun; Ye, Qian; Zeitlin, Andrew; Delaney, Colleen Suzanne, Co-culture of placental stem cells and stem cells from a second source.
Heidaran, Mohammad; Wang, Jia-Lun; Ye, Qian; Zeitlin, Andrew; Dulaney, Colleen Suzanne, Co-culture of placental stem cells and stem cells from a second source.
Emerson Stephen G. (Ann Arbor MI) Clarke Michael F. (Ann Arbor MI) Palsson Bernhard O. (Ann Arbor MI), Compositions containing cultured mitotic human stem cells.
Caplan Arnold I. ; Haynesworth Stephen E. ; Gerson Stanton L. ; Lazarus Hillard M., Enhancing hematopoietic progenitor cell engraftment using mesenchymal stem cells.
Bauer S. Christopher ; Abrams Mark Allen ; Braford-Goldberg Sarah Ruth ; Caparon Maire Helena ; Easton Alan Michael ; Klein Barbara Kure ; McKearn John Patrick ; Olins Peter O. ; Paik Kumnan ; Thomas, Ex-vivo expansion of stem cells using combinations of interleukin-3 (IL-3) variants and other cytokines.
Bjornson Christopher R. ; Rietze Rod L.,AUX ; Reynolds Brent A.,CAX ; Vescovi Angelo L.,ITX, Generation of hematopoietic cells from multipotent neural stem cells.
Nakahata Tatsutoshi (Matsumoto JPX) Kawano Genji (Kamakura JPX) Sudo Tetsuo (Kamakura JPX) Kojima Katsuaki (Yokohama JPX), Hematopoietic stem cell growth-promoting compositions containing a fibroblast-derived fragment of fibronectin and a grow.
Rubinstein Pablo ; Coelho Philip Henry ; Stevens Cladd E., High concentration white cells, a method for agglomeration of the high concentration and a bag set for use in conjuncti.
Janice M. Davis-Sproul ; Mark Aaron Moorman ; Renee Marie McNeil ; Donald William Simonetti, Jr. ; Lora Catherine Hammill ; Stewart Craig, Human mesenchymal stem cells.
Srour Edward (Indianapolis IN) Zanjani Esmail (Reno NV) Brandt John E. (Indianapolis IN) Hoffman Ronald (Indianapolis IN), Human stem cell compositions and methods.
Tsukamoto Ann (Palo Alto CA) Baum Charles M. (Mountain View CA) Aihara Yukoh (Yokohama JPX) Weissman Irving (Palo Alto CA), Identification and isolation of human hematopoietic stem cells.
Johnson R. Paul (Lexington MA) Rosenzweig Michael (Boston MA) Scadden David T. (Weston MA), In vitro differentiation of CD34+progenitor cells into T lymphocytes.
Weiss Samuel,CAX ; Reynolds Brent,CAX ; Hammang Joseph P. ; Baetge E. Edward, In vitro growth and proliferation of genetically modified multipotent neural stem cells and their progeny.
Prockop Darwin J. ; Pereira Ruth F. ; Leeper Dennis B. ; O'Hara Michael D., Isolated stromal cells for treating diseases, disorders or conditions characterized by bone defects.
Edward A. Boyse ; Hal E. Broxmeyer ; Gordon W. Douglas, Isolation and preservation of fetal and neonatal hematopoietic stem and progenitor cells of the blood.
Boyse Edward A. (Tucson AZ) Broxmeyer Hal E. (Indianapolis IN) Douglas Gordon W. (New York NY), Isolation and preservation of fetal and neonatal hematopoietic stem and progenitor cells of the blood and methods of the.
Pykett, Mark J.; Rosenzweig, Michael; Scadden, David T.; Poznansky, Mark C., Lymphoid tissue-specific cell production from hematopoietic progenitor cells in three-dimensional devices.
Kevin R. McIntosh ; Joseph D. Mosca ; Elena N. Klyushnenkova, Mesenchymal stem cells for prevention and treatment of immune responses in transplantation.
McIntosh Kevin R. ; Mosca Joseph D. ; Klyushnenkova Elena N., Mesenchymal stem cells for prevention and treatment of immune responses in transplantation.
McIntosh, Kevin R.; Mosca, Joseph D.; Klyushnenkova, Elena, Mesenchymal stem cells for prevention and treatment of immune responses in transplantation.
Caplan Arnold I. (1300 Oakridge Dr. Cleveland Heights OH 44121) Haynesworth Stephen E. (3643 Antisdale Rd. Cleveland Heights OH 44118), Method for enhancing the implantation and differentiation of marrow-derived mesenchymal cells.
Emerson Stephen G. (Ann Arbor MI) Clarke Michael F. (Ann Arbor MI) Palsson Bernhard O. (Ann Arbor MI), Method for the ex vivo replication of stem cells, for the optimization of hematopoietic progenitor cell cultur.
Caplan Arnold I. (1300 Oakridge Dr. Cleveland Heights OH 44121) Haynesworth Stephen E. (3643 Antisdale Rd. Cleveland Heights OH 44118), Method for treating connective tissue disorders.
Murray Lesley (San Jose CA) Sutherland D. Robert (Oakville CAX), Method of purifying a population of cells enriched for hematopoietic stem cells populations of cells obtained thereby an.
Emerson Stephen G. (Ann Arbor MI) Clarke Michael F. (Ann Arbor MI) Palsson Bernhard O. (Ann Arbor MI) Schwartz Richard M. (Ann Arbor MI), Methods and compositions for the ex vivo replication of human hematopoietic stem cells.
Emerson Stephen G. ; Clarke Michael F. ; Palsson Bernhard O., Methods and compositions for the ex vivo replication of stem cells, for the optimization of hematopoietic progenitor cell cultures, and for increasing the metabolism, GM-CSF secretion and/or IL-6 sec.
Fei Rui G. (Seattle WA) Heimfeld Shelly (Woodinville WA) Minshall Billy W. (Mill Creek WA) Berenson Ronald J. (Mercer Island WA), Methods and device for culturing human hematopoietic cells and their precursors.
Bodnar, Andrea G.; Chiu, Choy-Pik; Gold, Joseph D.; Inokuma, Margaret; Murai, James T.; West, Michael D., Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture.
Leon W. M. M. Terstappen ; Galla Chandra Rao ; Jonathan W. Uhr ; Emilian V. Racila ; Paul A. Liberti, Methods and reagents for the rapid and efficient isolation of circulating cancer cells.
Terstappen, Leon W. M. M.; Rao, Galla Chandra; Uhr, Jonathan W.; Racila, Emilian V.; Liberti, Paul A., Methods and reagents for the rapid and efficient isolation of circulating cancer cells.
Anderson David J. ; Shah Nirao M., Methods for differentiating neural stem cells to neurons or smooth muscle cells using TGT-.beta. super family growth factors.
Csete Marie ; Doyle John ; Wold Barbara, Methods for isolation and activation of, and control of differentiation from, skeletal muscle stem or progenitor cells.
Palsson Bernhard O. (Ann Arbor MI) Armstrong R. Douglas (Ann Arbor MI) Clarke Michael F. (Ann Arbor MI) Emerson Stephen G. (Ann Arbor MI), Methods for regulating the specific lineages of cells produced in a human hematopoietic cell culture.
Emerson Stephen G. (Ann Arbor MI) Clarke Michael F. (Ann Arbor MI) Palsson Bernhard O. (Ann Arbor MI), Methods for the ex vivo replication of human stem cells and/or expansion of human progenitor cells.
Hill Beth Louise ; Rozler Elen ; Chen Benjamin P., Methods of obtaining compositions enriched for hematopoietic stem cells, antibodies for use therein, compositions derived therefrom and methods of use thereof.
Emerson Stephen G. (Ann Arbor MI) Clarke Michael F. (Ann Arbor MI) Palsson Bernhard O. (Ann Arbor MI), Methods, compositions and devices for growing human hematopoietic cells.
Palsson Bernhard O. ; Emerson Stephen G. ; Schwartz Richard M., Methods, compositions and devices for maintaining and growing human stem and/or hematopoietics cells.
Hariri,Robert J.; Stirling,David I.; Moutouh De Parseval,Laure A.; Chan,Kyle W. H., Modulation of stem and progenitor cell differentiation, assays, and uses thereof.
Yannas Ioannis V. (Newton Center MA) Burke John F. (Belmont MA) Gordon Philip L. (Lexington MA) Huang Chor (Avon Lake OH), Multilayer membrane useful as synthetic skin.
Vyakarnam Murty N. ; Zimmerman Mark C. ; Scopelianos Angelo George ; Roller Mark B. ; Gorky David V., Porous tissue scaffoldings for the repair of regeneration of tissue.
Vyakarnam, Murty N.; Zimmerman, Mark C.; Scopelianos, Angelo George; Roller, Mark B.; Gorky, David V., Porous tissue scaffoldings for the repair or regeneration of tissue.
Boyse Edward A. (New York NY) Broxmeyer Hal E. (Indianapolis IN) Douglas Gordon W. (New York NY), Preservation of fetal and neonatal hematopoietic stem and progenitor cells of the blood.
Stern David M. (Great Neck NY) Oz Mehmet C. (Fort Lee NJ) Nowygrod Roman (Teaneck NJ) Koga Shin (New York NY) Pinsky David J. (Riverdale NY), Solution for prolonged organ preservation.
McIntosh, Kevin; Klyushnenkova, Elena, Suppressor cells induced by culture with mesenchymal stem cells for treatment of immune responses in transplantation.
Dumoulin Charles L. (Ballston Lake NY) Souza Steven P. (Williamstown MA) Darrow Robert D. (Scotia NY), Tracking system to monitor the position and orientation of a device using multiplexed magnetic resonance detection.
Gerson Stanton L. (Pepper Pike OH) Caplan Arnold I. (Cleveland Heights OH) Haynesworth Stephen E. (Cleveland Heights OH), Transduced mesenchymal stem cells.
Hessel Stephen R. (Fountain Valley CA) Young H. Theodore (Lake Forest CA) Katz Michael (Richmond CA), Umbilical cord clamping, cutting, and blood collecting device and method.
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