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A process for the production of glucose from a pretreated lignocellulosic feedstock is provided. The method comprises enzymatically hydrolyzing the pretreated lignocellulosic feedstock with cellulase enzymes to produce a hydrolyzate slurry comprising glucose and unhydrolyzed cellulose and fermenting

A process for the production of glucose from a pretreated lignocellulosic feedstock is provided. The method comprises enzymatically hydrolyzing the pretreated lignocellulosic feedstock with cellulase enzymes to produce a hydrolyzate slurry comprising glucose and unhydrolyzed cellulose and fermenting the hydrolyzate slurry in a fermentation reaction to produce a fermentation broth comprising alcohol. A process stream is obtained comprising unhydrolyzed cellulose, which is then subjected to a denaturing step, preferably comprising exposing the unhydrolyzed cellulose to elevated temperatures, thereby producing a heat-treated stream comprising the unhydrolyzed cellulose. The heat-treated stream comprising unhydrolyzed cellulose is then further hydrolyzed with cellulase enzymes to hydrolyze the cellulose to glucose.

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1. A process for increasing the hydrolysis of cellulose in the production of alcohol from a lignocellulosic feedstock, comprising the steps of: (i) pretreating a lignocellulosic feedstock under conditions to produce a composition comprising a pretreated lignocellulosic feedstock having a disrupted f

1. A process for increasing the hydrolysis of cellulose in the production of alcohol from a lignocellulosic feedstock, comprising the steps of: (i) pretreating a lignocellulosic feedstock under conditions to produce a composition comprising a pretreated lignocellulosic feedstock having a disrupted fiber structure and increased accessibility of the feedstock to being hydrolyzed;(ii) enzymatically hydrolyzing the pretreated lignocellulosic feedstock with cellulase enzymes to produce a hydrolyzate slurry comprising glucose and unhydrolyzed cellulose;(iii) fermenting said hydrolyzate slurry to produce a fermentation broth comprising alcohol and unhydrolyzed cellulose;(iv) separating the alcohol from the fermentation broth by distillation to obtain a stream comprising concentrated alcohol and a still bottoms stream comprising the unhydrolyzed cellulose and the cellulase enzymes, said distillation resulting in heat treatment of the unhydrolyzed cellulose, which heat treatment is conducted at a temperature between 70 and 200° C. so as to denature the cellulase enzymes; and(v) further hydrolyzing at least a portion of the still bottoms stream with cellulase enzymes to convert at least a portion of the unhydrolyzed cellulose present in said still bottoms stream to glucose, thereby increasing cellulose hydrolysis. 2. The process according to claim 1, wherein the step (v) of further hydrolyzing comprises recycling at least a portion of the still bottoms stream back to step (ii). 3. The process according to claim 1, wherein the step (v) of further hydrolyzing comprises introducing at least a portion of the still bottoms stream to a downstream hydrolysis reactor. 4. The process according to claim 1, wherein the step (ii) of enzymatically hydrolyzing the pretreated lignocellulosic feedstock and the step (iii) of fermenting said hydrolyzate slurry are carried out in separate reactors. 5. The process according to claim 1, wherein the step (i) of pretreatment is carried out with a pH adjustant. 6. The process according to claim 5, wherein the pH adjustant is acid or alkali. 7. The process according to claim 1, wherein the step (ii) of enzymatically hydrolyzing the pretreated lignocellulosic feedstock is carried out in a hydrolysis system comprising at least one hydrolysis reactor selected from the group consisting of agitated tanks, unmixed tanks, agitated towers and unmixed towers. 8. The process according to claim 1, wherein the step (ii) of enzymatically hydrolyzing is a batch process. 9. The process according to claim 1, wherein the alcohol produced in the step (iii) of fermenting is ethanol or butanol. 10. The process according to claim 9, wherein the distillation step (iv) is carried out with a slurry retention time of between about 0.05 and about 12 hours. 11. The process according to claim 10, wherein the temperatures of the distillation step (iv) are between about 70° C. and about 180° C. 12. The process according to claim 1, further comprising recycling at least a portion of the fermentation broth back to step (ii). 13. The process according to claim 1, wherein the concentrated still bottoms stream has a solids concentration of between 12 and 40% by weight. 14. A process for improving cellulose hydrolysis in the production of ethanol from a lignocellulosic feedstock, comprising the steps of: (i) pretreating a lignocellulosic feedstock under conditions to produce a composition comprising a pretreated lignocellulosic feedstock having a disrupted fiber structure and increased accessibility of the feedstock to being hydrolyzed;(ii) enzymatically hydrolyzing the pretreated lignocellulosic feedstock with cellulase enzymes to produce a hydrolyzate slurry comprising glucose and unhydrolyzed cellulose;(iii) fermenting said hydrolyzate slurry to produce a fermentation broth comprising ethanol;(iv) separating the ethanol from the fermentation broth by distillation to obtain a stream comprising concentrated ethanol and a remaining still bottoms stream comprising unhydrolyzed cellulose and the cellulase enzymes, said distillation resulting in heat treatment of the unhydrolyzed cellulose, which heat treatment is conducted at temperatures between 70 and 200° C. so as to denature the cellulase enzymes;(v) further hydrolyzing at least a portion of the still bottoms stream with cellulase enzymes to convert the unhydrolyzed cellulose to glucose by recycling at least a portion of the still bottoms stream back to step (ii), thereby increasing cellulose hydrolysis; and(vi) subsequent to the distillation, obtaining a concentrated ethanol product. 15. The process according to claim 14, wherein the concentrated still bottoms stream has a solids concentration of between 12 and 40% by weight. 16. A process for producing glucose from a lignocellulosic feedstock, comprising the steps of: (i) pretreating the lignocellulosic feedstock under conditions to produce a pretreated lignocellulosic feedstock having disrupted fiber structure and increased accessibility of the lignocellulosic feedstock to being hydrolyzed;(ii) enzymatically hydrolyzing the pretreated lignocellulosic feedstock with cellulase enzymes to produce a hydrolyzate slurry comprising glucose and unhydrolyzed cellulose and fermenting the glucose to produce a fermentation broth comprising alcohol;(iii) obtaining at least a portion of the hydrolyzate slurry comprising the unhydrolyzed cellulose;(iv) subjecting at least a portion of said hydrolyzate slurry to a processing step comprising exposing the unhydrolyzed cellulose to a temperature of between about 70° C. and about 250° C., thereby producing a hydrolyzate slurry comprising heat-treated unhydrolyzed cellulose; and(v) further hydrolyzing the heat-treated unhydrolyzed cellulose in said hydrolyzate slurry with cellulase enzymes to convert at least a portion of the unhydrolyzed cellulose to glucose. 17. The process according to claim 16, wherein the pretreatment step (i) is carried out with a pH alterant. 18. The process according to claim 17, wherein the pH alterant is acid or alkali.