[특허]Isotonic buffered composition and method that enables counting of cells

Beckman Coulter, Inc.

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특허 상세정보

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Isotonic buffered composition and method that enables counting of cells

특허상세정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	G01N-033/50
출원번호	US-0853046 (2010-08-09)
등록번호	US-9091677 (2015-07-28)
발명자 / 주소	Goldson, Wayne Socarras, Sandra
출원인 / 주소	Beckman Coulter, Inc.
대리인 / 주소	K&L Gates LLP
인용정보	피인용 횟수 : 1 인용 특허 : 40

초록

The present invention discloses multi-purpose metering fluid/rinse reagents for use in automated cellular analyzers that use liquid volumetric metering. The compositions contain a chelating agent, an ionizing salt, optionally a stabilizing ion, a buffer, a non-hemolytic surfactant, and optionally an antimicrobial agent. Advantageously, the compositions produce less than one part-per-million of formaldehyde over the course of one year. Methods for using the compositions are also described.

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1. A composition for use in an automated cellular analyzer that uses liquid volumetric metering, the composition comprising: a) a chelating agent present at a concentration of about 2.7 g/l to about 3.3 g/L,b) an ionizing salt, andc) a non-hemolytic surfactant present at a concentration of about 0.1 g/L to about 3.0 g/L,wherein the composition is non-hemolytic, has a pH of about 6.5 to about 7.5, has less than 1 part-per-million of formaldehyde over a period of at least one year, and wherein the surface tension of the composition is between about 40 dynes/cm and about 66 dynes/cm. 2. The composition of claim 1, wherein the composition is in a container configured to be connected to the automated cellular analyzer. 3. The composition of claim 1, wherein the chelating agent is ethylenediamine tetraacetic acid (EDTA), disodium EDTA, dipotassium EDTA, ethyleneglycol-bis-(2-aminoethylether)N,N,N′,N′-tetraacetic acid (EGTA), or combinations thereof. 4. The composition of claim 1, wherein the ionizing salt is an alkaline metal chloride. 5. The composition of claim 4, wherein the alkaline metal chloride is sodium chloride, potassium chloride, or combinations thereof. 6. The composition of claim 1, further comprising a stabilizing ion. 7. The composition of claim 6, wherein the stabilizing ion is provided by an alkaline metal sulfate. 8. The composition of claim 7, wherein the alkaline metal sulfate is sodium sulfate, potassium sulfate, or combinations thereof. 9. The composition of claim 1, wherein the non-hemolytic surfactant is polyethoxylated nonyl phenol, polyoxyethylene 9 lauryl ether, ethoxylated glycol ether, ethoxylated monododecyl ether, ethoxylated dimethylsiloxane, dodecyl nonaethylene glycol ether, or combinations thereof. 10. The composition of claim 1, further comprising one or more antimicrobial agents. 11. The composition of claim 10, wherein the antimicrobial is 1,2-benzisothiazolin-3-one, 5-chloro-2-methyl-4-isothiazolin-3-one,2-methyl-4-isothiazolin-3-one, 4-butylaminobenzoicacid-2-(dimethyl amino) ethylester hydrochloride, 5-bromo-5-nitro-1,3-dioxane, 1,2 dibromo-2,4 dicyanobutane, or combinations thereof. 12. The composition of claim 1, wherein the surface tension of the composition is between about 47 dynes/cm and about 64 dynes/cm. 13. The composition of claim 1, wherein the surface tension of the composition is between about 52 dynes/cm and about 62 dynes/cm. 14. A composition for use in an automated cellular analyzer that uses liquid volumetric metering, the composition comprising: a) a chelating agent present at a concentration of about 2.7 g/l to about 3.3 g/L,b) an ionizing salt, andc) a non-hemolytic surfactant, present at a concentration of about 0.1 g/L to about 3.0 g/L,wherein the composition is non-hemolytic, has a pH of about 6.5 to about 7.5, has less than 1 part-per-million of formaldehyde over a period of at least one year, and wherein the composition produces a meniscus with an angle between about 30° and about 60° in a borosilicate glass capillary with an internal diameter of 2.7 mm. 15. A composition for use in an automated cellular analyzer that uses liquid volumetric metering, the composition comprising: a) a chelating agent present at a concentration of about 2.7 g/L to about 3.3 g/L,b) an ionizing salt present at a concentration of about 0.1 g/L to about 5 g/L, andc) a non-hemolytic surfactant present at a concentration of about 0.1 g/L to about 3.0 g/L,wherein the composition is non-hemolytic, has a pH of about 6.5 to about 7.5, has less than 1 part-per-million of formaldehyde over a period of at least one year, and wherein the composition produces a meniscus with an angle between about 30° and about 60° when flowing through a metering tube. 16. A composition for use in an automated cellular analyzer that uses liquid volumetric metering, the composition comprising: a) a chelating agent present at a concentration of about 2.7 g/L to about 3.3 g/L,b) an ionizing salt present at a concentration of about 0.5 g/L to about 1.5 g/L, and c) a non-hemolytic surfactant present at a concentration of about 0.9 g/L to about 2.1 g/L,wherein the composition is non-hemolytic and has a pH of about 6.5 to about 7.5has less than 1 part-per-million of formaldehyde over a period of at least one year. 17. A composition for use in an automated cellular analyzer that uses liquid volumetric metering, the composition comprising: a) a chelating agent present at a concentration of about 2.7 g/L to about 3.3 g/L,b) an ionizing salt, andc) a non-hemolytic surfactant present at a concentration of about 0.1 g/L to about 3.0 g/L,wherein the composition is non-hemolytic, has a pH of about 6.5 to about 7.5, has less than about 1 part-per-million of formaldehyde over the course of its shelf life, and wherein the surface tension of the composition is between about 40 dynes/cm and about 66 dynes/cm. 18. A composition for use in an automated cellular analyzer that uses liquid volumetric metering, the composition comprising: a) a chelating agent present at a concentration of about 2.7 g/L to about 3.3 g/L,b) an ionizing salt, andc) a non-hemolytic surfactant present at a concentration of about 0.1 g/L to about 3.0 g/L,wherein the composition is non-hemolytic, has a pH of about 6.5 to about 7.5, has less than 1 part-per-million of formaldehyde over the course of its shelf life, and wherein the composition produces a meniscus with an angle between about 30° and about 60° in a borosilicate glass capillary with an internal diameter of 2.7 mm. 19. A method for controlling the start and stop of a counting episode on an automated cellular analyzer that uses liquid volumetric metering, the method comprising: a) providing to a metering tube a metering fluid composition comprising: i) a chelating agent, present at a concentration of about 2.7 g/L to about 3.3 g/L,ii) an ionizing salt, andiii) a non-hemolytic surfactant present at a concentration of about 0.1 g/L to about 3.0 g/L;wherein the composition is non-hemolytic, has a pH of about 6.5 to about 7.5, and wherein the surface tension of the composition is between about 40 dynes/cm and about 66 dynes/cm;b) flowing the metering fluid composition through the metering tube; wherein the meniscus of the metering fluid first triggers the start sensor, beginning the counting episode, and then triggers the end sensor, stopping the counting episode; andc) wherein said composition has less than 1 part-per-million of formaldehyde over a period of at least one year. 20. The method of claim 19, wherein the surface tension of the composition is between about 47 dynes/cm and about 64 dynes. 21. The method of claim 19, wherein the surface tension of the composition is between about 52 dynes/cm and about 62 dynes/cm. 22. A method for controlling the start and stop of a counting episode on an automated cellular analyzer that uses liquid volumetric metering, the method comprising: a) providing to a metering tube a metering fluid composition according to claim 1;b) flowing the metering fluid composition through the metering tube; wherein the meniscus of the metering fluid first triggers the start sensor, beginning the counting episode, and then triggers the end sensor, stopping the counting episode. 23. The method of claim 22, wherein the surface tension of the composition is between about 47 dynes/cm and about 64 dynes. 24. The method of claim 22, wherein the surface tension of the composition is between about 52 dynes/cm and about 62 dynes/cm.

이 특허에 인용된 특허 (40)

이 특허를 인용한 특허 피인용횟수: 1

Goldson, Wayne; Socarras, Sandra. Isotonic buffered composition and method that enables counting of cells. USP2017029567622.

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