초록 ▼

A substituted Norovirus capsid protein monomer, having only the P-domain, includes a foreign antigen inserted into one or more of three surface loops present on each P-domain monomer by molecular cloning. The antigen-P-domain monomer can assemble spontaneously into an octahedral, antigen-Norovirus P

A substituted Norovirus capsid protein monomer, having only the P-domain, includes a foreign antigen inserted into one or more of three surface loops present on each P-domain monomer by molecular cloning. The antigen-P-domain monomer can assemble spontaneously into an octahedral, antigen-Norovirus P-particle, composed of 24 copies of the monomer. Each substituted P-domain monomer can contain one to three copies of the foreign antigen, for a total of 24-72 antigen copies on each antigen-P-particle. The antigen-P-particle is useful in methods for diagnosing, immunizing and treating individuals infected with a foreign virus and as a carrier for development of vaccines against many infectious and non-infectious diseases. The substituted monomer can be readily produced in E. coli and yeast, are highly stable and tolerate a wide range of physio-chemical conditions. The P-particle-VP8 chimeras may also serve as a dual vaccine, for example, against both rotavirus and norovirus.

대표청구항 ▼

1. A method of inducing an immune response in a mammal against a viral antigen, comprising the step of administering to the mammal an effective amount of an immunogenic composition comprising an antigen-P-particle consisting of 24 monomers, said P-particle including at least one antigen-P-domain mon

1. A method of inducing an immune response in a mammal against a viral antigen, comprising the step of administering to the mammal an effective amount of an immunogenic composition comprising an antigen-P-particle consisting of 24 monomers, said P-particle including at least one antigen-P-domain monomer, wherein the antigen-P-particle can enhance the immunogenicity of the antigen as compared to said antigen along; wherein said at least one antigen P-domain monomer comprises a Norovirus (NOR) P-domain monomer having at least one surface loop and a foreign protein or polypeptide antigen inserted into said at least one surface loop. 2. The method according to claim 1, wherein the foreign protein or polypeptide antigen is rotovirus (RV) VP8 antigen, thereby forming a P-particle-VP8 chimera, which serves as an immunogenic composition against both rotavirus and norovirus.