초록 ▼

This invention provides a method for testing a factor for cardiogenicity which comprises differentiating human embryonic stem (hES) cells to cardiomyocytes in the presence and absence of the factor wherein the human embryonic stem (hES) cells are cultured under a serum free condition comprising co-c

This invention provides a method for testing a factor for cardiogenicity which comprises differentiating human embryonic stem (hES) cells to cardiomyocytes in the presence and absence of the factor wherein the human embryonic stem (hES) cells are cultured under a serum free condition comprising co-culture in the presence of END-2 cells or serum-free extracellular medium therefrom, and measuring the differentiation in the presence and absence of the factor. This invention also provides a method for identifying a cardiogenic factor, which comprises differentiating human embryonic stem (hES) cells to cardiomyocytes in the presence or absence of the factor wherein the human embryonic stem (hES) cells are cultured under a serum free condition comprising co-culture in the presence of END-2 cells or serum-free extracellular medium therefrom, and identifying the factor that affects the differentiation of human embryonic stem (hES) cells to cardiomyocytes in the presence or absence of the factor.

대표청구항 ▼

1. A method for testing a factor for cardiogenicity, said method comprising: a) differentiating human embryonic stem (hES) cells to cardiomyocytes in the presence and absence of the factor wherein the hES cells are cultured under a serum free condition comprising co-culture in the presence of mouse

1. A method for testing a factor for cardiogenicity, said method comprising: a) differentiating human embryonic stem (hES) cells to cardiomyocytes in the presence and absence of the factor wherein the hES cells are cultured under a serum free condition comprising co-culture in the presence of mouse visceral endoderm-like (END-2) cells or serum free extracellular medium therefrom, and b) measuring the differentiation of hES cells to cardiomyocytes in the presence and absence of the factor, wherein improved efficiency of differentiation of hES cells to cardiomyocytes in the presence of the factor indicates the factor is cardiogenic. 2. A method for testing a factor for efficiency of differentiation of human embryonic stem (hES) cells to cardiomyocytes over serum containing conditions, in vitro, the method comprising: a) co-culturing the hES cells with mouse visceral endoderm-like (END-2) cells or with an extracellular medium therefrom, in the presence and absence of the factor under serum free conditions that induce differentiation and measuring the efficiency of differentiation in the presence and absence of the factor; and b) comparing the differentiation of hES cells to cardiomyocytes from a) with the differentiation of hES cells to cardiomyocytes in the presence and absence of the factor under serum containing conditions. 3. The method according to claim 2 wherein the efficiency of differentiation of hES cells to cardiomyocytes is measured from 5 days of co-culture with the mouse visceral endoderm-like (END-2) cells or with an extracellular medium therefrom.