Moenomycin biosynthesis-related compositions and methods of use thereof
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C07K-014/00
C12N-015/52
출원번호
US-0377117
(2007-08-13)
등록번호
US-9115358
(2015-08-25)
국제출원번호
PCT/US2007/017999
(2007-08-13)
§371/§102 date
20100518
(20100518)
국제공개번호
WO2008/021367
(2008-02-21)
발명자
/ 주소
Walker, Suzanne
Omelyanovich Ostash, Bohdan
출원인 / 주소
President and Fellows of Harvard College
대리인 / 주소
Wolf, Greenfield & Sacks, P.C.
인용정보
피인용 횟수 :
2인용 특허 :
55
초록▼
The methods and compositions described herein relate to the identification, isolation, and characterization of genes which encode proteins useful for the biosynthesis of transglycosylase inhibitors such as moes. The methods and compositions also relate to the production of such proteins, and their u
The methods and compositions described herein relate to the identification, isolation, and characterization of genes which encode proteins useful for the biosynthesis of transglycosylase inhibitors such as moes. The methods and compositions also relate to the production of such proteins, and their use in the synthesis of moes, the expression of moes, and the production of modified moes.
대표청구항▼
1. An isolated recombinant cell expressing one or more polypeptides that comprise a moenomycin biosynthesis-related polypeptide selected from the group consisting of: MoeA4 (SEQ ID NO: 29), MoeB4 (SEQ ID NO: 30), MoeC4 (SEQ ID NO: 28), MoeB5 (SEQ ID NO: 27), Moe A5 (SEQ ID NO: 26), MoeD5 (SEQ ID NO:
1. An isolated recombinant cell expressing one or more polypeptides that comprise a moenomycin biosynthesis-related polypeptide selected from the group consisting of: MoeA4 (SEQ ID NO: 29), MoeB4 (SEQ ID NO: 30), MoeC4 (SEQ ID NO: 28), MoeB5 (SEQ ID NO: 27), Moe A5 (SEQ ID NO: 26), MoeD5 (SEQ ID NO: 47), MoeJ5 (SEQ ID NO: 48), MoeE5 (SEQ ID NO: 42), MoeF5 (SEQ ID NO: 36), MoeH5 (SEQ ID NO: 37), MoeK5 (SEQ ID NO: 38), MoeM5 (SEQ ID NO: 39), MoeN5 (SEQ ID NO: 43), MoeO5 (SEQ ID NO: 44), MoeX5 (SEQ ID NO: 46), MoeP5 (SEQ ID NO: 45), MoeR5 (SEQ ID NO: 40), MoeS5 (SEQ ID NO: 41), MoeGT1 (SEQ ID NO: 31), MoeGT2 (SEQ ID NO: 32), MoeGT3 (SEQ ID NO: 33), MoeGT4 (SEQ ID NO: 34), MoeGT5 (SEQ ID NO: 35), and variants thereof having at least about 95% sequence identity to the corresponding natural moenomycin biosynthesis-related polypeptide, wherein the cell is selected from the group consisting of: Streptomyces lividans TK24, E. coli, a mammalian cell, a yeast cell, and an insect cell. 2. The isolated recombinant cell of claim 1, wherein the cell is Streptomyces lividans TK24. 3. The isolated recombinant cell of claim 1, wherein the cell is E. coli. 4. The isolated recombinant cell of claim 1, wherein the cell is a mammalian cell. 5. The isolated recombinant cell of claim 1, wherein the cell is a yeast cell. 6. The isolated recombinant cell of claim 1, wherein the cell is an insect cell. 7. The isolated recombinant cell of claim 1, wherein the cell expresses two or more polypeptides that comprise the moenomycin biosynthesis-related polypeptide and variants thereof. 8. The isolated recombinant cell of claim 1, wherein the cell expresses three or more polypeptides that comprise the moenomycin biosynthesis-related polypeptide and variants thereof. 9. The isolated recombinant cell of claim 1, wherein the cell expresses four or more polypeptides that comprise the moenomycin biosynthesis-related polypeptide and variants thereof. 10. The isolated recombinant cell of claim 1, wherein the cell expresses five or more polypeptides that comprise the moenomycin biosynthesis-related polypeptide and variants thereof. 11. The isolated recombinant cell of claim 1, wherein the moenomycin biosynthesis-related polypeptide variants have at least about 98% sequence identity to the corresponding natural moenomycin biosynthesis-related polypeptide. 12. The isolated recombinant cell of claim 1, wherein the moenomycin biosynthesis-related polypeptide variants have at least about 99% sequence identity to the corresponding natural moenomycin biosynthesis-related polypeptide.
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