[미국특허]
Methods and kits for linking polymorphic sequences to expanded repeat mutations
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12Q-001/68
C07H-021/04
C12N-015/113
출원번호
US-0752789
(2007-05-23)
등록번호
US-9273356
(2016-03-01)
발명자
/ 주소
van Bilsen, Paul
Kaemmerer, William F.
Burright, Eric
출원인 / 주소
Medtronic, Inc.
대리인 / 주소
Fox Rothschild LLP
인용정보
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0인용 특허 :
61
초록▼
Methods and kits are provided for determining which single nucleotide polymorphism (“SNP”) variant of an allele of a heterozygous patient is on the same allele as a disease-causing mutation that is at a remote region of the gene's mRNA comprising a) an allele specific reverse transcription reaction
Methods and kits are provided for determining which single nucleotide polymorphism (“SNP”) variant of an allele of a heterozygous patient is on the same allele as a disease-causing mutation that is at a remote region of the gene's mRNA comprising a) an allele specific reverse transcription reaction using an allele specific primer which recognizes one SNP variant, wherein further the 3′ end of the primer is positioned at the SNP nucleotide position, and b) analysis of the resulting cDNA product from the reverse transcription reaction at the region of the mutation to determine the presence or absence of the mutation on this allele specific cDNA product, wherein the allele specific primer is shorter than about 20 nucleotides.
대표청구항▼
1. A method for determining which single nucleotide polymorphism variant of an allele from a gene isolated from a heterozygous patient is on the same mRNA transcript as a disease-causing mutation at a remote region of the gene's mRNA comprising: a) providing first allele-specific primer which recogn
1. A method for determining which single nucleotide polymorphism variant of an allele from a gene isolated from a heterozygous patient is on the same mRNA transcript as a disease-causing mutation at a remote region of the gene's mRNA comprising: a) providing first allele-specific primer which recognizes a single nucleotide polymorphism (SNP) variant of a SNP in the mRNA transcript of said heterozygous patient,b) providing a second allele-specific primer which recognizes another variant of said SNP in the mRNA transcript of said heterozygous patient,c) conducting a reverse transcription reaction from said gene's mRNA using either the allele-specific primer of part (a) or the allele-specific primer of part (b);d) providing a set of PCR primers flanking said disease-causing mutation and capable of hybridizing to the allele-specific cDNA product, ande) amplifying said remote region of the allele-specific cDNA product using a said set of PCR primers,wherein the allele-specific primer and the second allele-specific primer is shorter than 18 nucleotides. 2. The method of claim 1, wherein the 3′ end of the allele-specific primer is positioned at the single nucleotide polymorphism nucleotide position. 3. The method of claim 1 wherein the 3′ end of at least the second allele-specific primer is positioned at the single nucleotide polymorphism nucleotide position. 4. The method of claim 1 wherein the 3′ end of at least the second allele-specific primer is positioned at the single nucleotide polymorphism nucleotide position. 5. A method for determining which single nucleotide polymorphism (SNP) variant of an allele from a gene isolated from a heterozygous patient is on the same mRNA transcript as a disease-causing mutation at a remote region of the gene's mRNA comprising (a) providing a first allele-specific primer which recognizes a first variant of a first SNP in the mRNA transcript of said heterozygous patient,(b) providing a second allele-specific primer which recognizes a second variant of said first SNP in the mRNA transcript of said heterozygous patient,(c) conducting a first reverse transcription reaction from said gene's mRNA using the allele-specific primer of part (a) to obtain a first subpopulation of allele-specific cDNA product or conducting a second reverse transcription reaction from said gene's mRNA using the allele-specific primer of part (b) to obtain a second subpopulation of allele-specific cDNA product, wherein the first subpopulation and second subpopulation are allele-specific for said first variant and said second variant, respectively;(d) providing a set of PCR primers flanking said disease-causing mutation and capable of hybridizing to said first or second subpopulation of allele-specific cDNA product, and(e) amplifying said remote region of the allele-specific cDNA product using said set of PCR primers to obtain a PCR product, wherein the first allele-specific primer and the second allele-specific primer is shorter than 18 nucleotides, and further comprising at least(f) providing a third allele-specific primer which recognizes one variant of a second single nucleotide polymorphism (second SNP), wherein said second SNP is different from said first SNP,(g) providing a fourth allele-specific primer which recognizes another variant of said second SNP,(h) conducting a reverse transcription reaction from said gene's mRNA using either the third allele-specific primer of part (f) or the fourth allele-specific primer of part (g) thereby generating an allele-specific cDNA product; and(i) amplifying said remote region of the allele-specific cDNA product of part (h) using said set of PCR primers,wherein the third allele-specific primer and the fourth allele-specific primer are shorter than 18 nucleotides. 6. The method of claim 5, wherein the said disease is Huntington's disease, and both of said first SNP and said second SNP are selected from rs1936032, rs4690074, rs363125, and rs362331. 7. The method of claim 5, further comprising at least: (j) providing a fifth allele-specific primer which recognizes one variant of a third single nucleotide polymorphism (third SNP), wherein said third SNP is different from the single nucleotide polymorphism and the second SNP,(k) providing a sixth allele-specific primer which recognizes another variant of said third SNP,(l) conducting a reverse transcription reaction from said gene's mRNA using either the allele-specific primer of part (j) or the allele-specific primer of part (k) thereby generating an allele-specific cDNA product; and(m) amplifying said remote region of the allele-specific cDNA product of part (l) using said set of PCR primers,wherein the fifth allele-specific primer and the sixth allele-specific primer is shorter than 18 nucleotides. 8. The method of claim 7, wherein the said disease is Huntington's disease, and said SNP, said second SNP and said third SNP are selected from rs1936032, rs4690074, rs363125, and rs362331. 9. The method of claim 8, further comprising: (n) providing a seventh allele-specific primer which recognizes one variant of a fourth single nucleotide polymorphism (fourth SNP), wherein said fourth SNP is different from the single nucleotide polymorphism, the second SNP, and the third SNP,(o) providing an eighth allele-specific primer which recognizes another variant of said fourth SNP,(p) conducting a reverse transcription reaction from said gene's mRNA using either the allele-specific primer of part (n) or the allele-specific primer of part (o) thereby generating an allele-specific cDNA product; and(q) amplifying said remote region of the allele-specific cDNA product of part (p) using said set of PCR primers,wherein the seventh allele-specific primer and the eighth allele-specific primer is shorter than 18 nucleotides. 10. The method of claim 9, wherein the said disease is Huntington's disease, and said SNP, said second SNP, said third SNP and said fourth SNP are selected from rs1936032, rs4690074, rs363125, and rs362331. 11. The method of claim 5, wherein step (h) is carried out by conducting a third reverse transcription reaction from said gene's mRNA using the third allele-specific primer of part (f) to obtain a third subpopulation of allele-specific cDNA product or a fourth reverse transcription reaction from said gene's mRNA using the fourth allele-specific primer of part (g) to obtain a fourth subpopulation of allele-specific cDNA product. 12. The method of claim 5, wherein said remote region is in the 5′ direction from the site of said first SNP in the gene's mRNA. 13. The method of claim 12, wherein said remote region is at least 6.5 kb or more from the site of said first SNP. 14. The method of claim 5, wherein the PCR product obtained from step (e) does not include the site of said first SNP. 15. The method of claim 5, wherein step (c) is carried out by conducting said first reverse transcription reaction and conducting said second reverse transcription reaction in separate tubes.
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