[미국특허]
Chemotherapeutic methods and compositions
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A61K-039/395
A61K-031/7105
A61K-031/282
A61K-031/337
A61K-031/513
A61K-031/517
A61K-031/519
A61K-031/704
A61K-031/7068
A61K-033/24
A61K-045/06
C07K-016/40
C12N-015/113
출원번호
US-0619139
(2012-09-14)
등록번호
US-9289447
(2016-03-22)
발명자
/ 주소
Smith, Victoria
Holzer, Alison Kay
출원인 / 주소
Gilead Biologics, Inc.
대리인 / 주소
Dammann, Yu-Ming
인용정보
피인용 횟수 :
0인용 특허 :
72
초록▼
Disclosed herein are methods and compositions for enhancing the cell-killing activity of anti-neoplastic agents by inhibiting the activity of a lysyl oxidase-type enzyme. Also disclosed are methods for screening for chemotherapeutic agents, and for molecules that enhance the activity of chemotherape
Disclosed herein are methods and compositions for enhancing the cell-killing activity of anti-neoplastic agents by inhibiting the activity of a lysyl oxidase-type enzyme. Also disclosed are methods for screening for chemotherapeutic agents, and for molecules that enhance the activity of chemotherapeutic agents, using cells grown on an extracellular matrix.
대표청구항▼
1. A method for killing a malignant cell, the method comprising: contacting the malignant cell, wherein the malignant cell expresses a lysyl oxidase-type enzyme, with an anti-neoplastic agent and an inhibitor of the lysyl oxidase-type enzyme, wherein the cell is growing in the presence of an extrace
1. A method for killing a malignant cell, the method comprising: contacting the malignant cell, wherein the malignant cell expresses a lysyl oxidase-type enzyme, with an anti-neoplastic agent and an inhibitor of the lysyl oxidase-type enzyme, wherein the cell is growing in the presence of an extracellular matrix containing collagen, whereby the malignant cell is killed, wherein the anti-neoplastic agent is a chemotherapeutic agent and the inhibitor is an isolated lysyl oxidase-like 2 (LOXL2) antibody or antigen-binding fragment thereof specifically binds to an epitope within the amino acid sequence of SEQ ID NO: 28; wherein the antibody or antigen-binding fragment thereof comprises a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 14 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 15. 2. The method of claim herein the malignant cell is in culture. 3. The method of claim 1, wherein the malignant cell is present in a tumor. 4. The method of claim 1, wherein the chemotherapeutic agent is selected from the group consisting of methotrexate, cispiatin, doxorubicin, docetaxel, erlotinih, paciitaxel, paraclitaxel, 5-fluorouracil and germcitibine. 5. The method of claim 1, wherein the collagen comprises type 1 collagen. 6. The method of claim 1, wherein the LOXL2 antibody or antigen-binding fragment thereof is a Fv, a scFv, a Fab, or a F(ab′)2. 7. The method of claim 1, wherein the LOXL2 inhibitor inhibits LOXL2 enzymatic activity. 8. The method of claim 1, wherein the LOXL2 antibody or antigen-binding fragment thereof is polyclonal. 9. The method of claim 1, wherein the antibody or antigen-binding fragment thereof is monoclonal. 10. The method of claim 9, wherein the antibody or antigen-binding fragment thereof is humanized. 11. The method of claim 1, wherein the antibody or antigen-binding fragment thereof comprises the complementarity determining regions CDRs), CDR1, CDR2, and CDR3, of a heavy chain variable region comprising the amino acid sequence of SEQ ID NO; 14, and the CDRs, CDR1, CDR2, and CDR3, of a light chain variable region comprising the amino acid sequence of SEQ ID NO: 15. 12. The method of claim 11, wherein the antibody or antigen-binding fragment thereof comprises a heavy chain variable region comprising the amino acid sequence of SEC) ID NO: 14. 13. The method of claim 11, wherein the antibody or antigen-binding fragment thereof comprises a light chain variable region comprising the amino acid sequence of SEQ ID NO; 15. 14. The method of claim 1, wherein the malignant cell is selected from the group consisting of fibrosarcoma, breast carcinoma, breast adenocarcinoma, pancreatic adenocarcinoma, pancreatic tumor, colon adenocarcinoma, ovarian adenocarcinoma, and lung carcinoma.
Kagan Herbert M. (Arlington MA) Gacheru Stephen N. (Worcester MA), Anti-fibrotic agents and methods for inhibiting the activity of lysyl oxidase in-situ using adjacently positioned diamin.
Garcia Fernando S. (Eden Prairie MN) Ginnow-Merkert Hartmut (Eden Prairie MN) Anderson Paul J. (Eden Prairie MN) Hudson Bertram J. (Eden Prairie MN), Glucose medical monitoring system.
Wands Jack R. (Waban MA) Zurawski ; Jr. Vincent R. (West Chester PA) Schoemaker ; Hubert J. P. (Newton MA), Immunoassay utilizing monoclonal high affinity IgM antibodies.
McCarthy James R. (West Chester OH) Barney Charlotte L. (Cincinnati OH) Matthews Donald P. (West Chester OH) Bey Philippe (Cincinnati OH), Inhibitors of lysyl oxidase.
Evans,Mark J.; Scicchitano,Marshall S.; Bapat,Ashok R.; Bhat,Ramesh A.; Mastroeni,Robert; Karathanasis,Sotirios K., Member of the lysyl oxidase gene family.
Evans,Mark J.; Scicchitano,Marshall S.; Bapat,Ashok R.; Bhat,Ramesh A.; Mastroeni,Robert; Karathanasis,Sotirios K., Member of the lysyl oxidase gene family.
Smulson Mark E. (Washington DC) Bhatia Kishor (Arbutus MD) Huppi Konrad (Reston VA), Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for hu.
Kim, Byung chul; Kim, Jhin gook; Hur, Nam; Lee, Kyu sang; Son, Dae soon; Park, Kyung hee; Ahn, Tae jin, Method of predicting risk of lung cancer recurrence, and a composition, kit and microarray for the same.
Smith, Victoria; Ogg, Scott; Van Vlasselaer, Peter; Barry, Vivian E.; Marshall, Derek; Holzer, Alison Kay; Rodriguez, Hector; Oyasu, Miho; McCauley, Scott Alan; Garcia, Carlos Aurelio; Biermann, Donna Hiroko Tokuoka, Methods and compositions for treatment and diagnosis of fibrosis, tumor invasion, angiogenesis, and metastasis.
Smith, Victoria; Ogg, Scott; Van Vlasselaer, Peter; Barry, Vivian E.; Marshall, Derek; Holzer, Alison Kay; Rodriguez, Hector; Oyasu, Miho; McCauley, Scott Alan; Garcia, Carlos Aurelio; Biermann, Donna Hiroko Tokuoka, Methods and compositions for treatment and diagnosis of fibrosis, tumor invasion, angiogenesis, and metastasis.
Hung Mien-Chie (Houston TX) Yu Di-Hua (Houston TX) Matin Angabin (Houston TX), Methods for the suppression of neu mediated tumors by adenoviral E1A and SV40 large T antigen.
Simonsson Leif R. (Hisings Backa SEX) Arielly Salo (Kungsbacka SEX) Aurell Leif E. (SrSEX) Claeson Karl G. (Linding SEX), Peptide substrates for determination of protease activity.
Neufeld, Gera; Akiri, Gal; Vadasz, Zahava; Gengrinovitch, Stela, Pharmaceutical compositions and methods useful for modulating angiogenesis, inhibiting metastasis and tumor fibrosis, and assessing the malignancy of colon cancer tumors.
Surani Azim M. (Cambridge GB3) Neuberger Michael S. (Cambridge GB3) Bruggemann Marianne (Cambridge GB3), Production of antibodies from transgenic animals.
Cabilly Shmuel (Monrovia CA) Heyneker Herbert L. (Burlingame CA) Holmes William E. (Pacifica CA) Riggs Arthur D. (La Verne CA) Wetzel Ronald B. (San Francisco CA), Recombinant immunoglobin preparations.
Cox, III, George Norbert; Case, Casey Christopher; Eisenberg, Stephen P.; Jarvis, Eric Edward; Spratt, Sharon Kaye, Regulation of endogenous gene expression in cells using zinc finger proteins.
Kouvonen Ilkka Sakari,FIX ; Svens Eivor Helena,FIX ; Tikanoja Sari Hannele,FIX ; Turunen Pekka Antero,FIX ; Sivonen Lauri Markus,FIX, Test strip, its production and use.
Griss ; deceased Gerhart (late of Biberach DEX by Elisabeth Griss ; legal representative) Hurnaus Rudolf (Biberach DEX) Kobinger Walter (Vienna ATX) Pichler Ludwig (Vienna ATX) Bauer Rudolf (Wiesbade, Tetrahydro-benzthiazoles, the preparation thereof and their use as intermediate products or as pharmaceuticals.
Griss ; deceased Gerhart (late of Biberach DEX by Elisabeth Griss ; legal representative) Schneider Claus (Ingelheim am Rhein DEX) Hurnaus Rudolf (Biberach DEX) Kobinger Walter (Vienna ATX) Pichler L, Tetrahydro-benzthiazoles, the preparation thereof and their use as intermediate products or as pharmaceuticals.
Griss ; deceased Gerhart (late of Biberach DEX by Elisabeth Griss ; legal representative) Schneider Claus (Ingelheim am Rhein DEX) Hurnaus Rudolf (Biberach DEX) Kobinger Walter (Vienna ATX) Pichler L, Tetrahydro-benzthiazoles, the preparation thereof and their use as intermediate products or as pharmaceuticals.
Lonberg Nils (San Francisco CA) Kay Robert M. (San Francisco CA), Transgenic non-human animals capable of producing heterologous antibodies of various isotypes.
Lonberg Nils (San Francisco CA) Kay Robert M. (San Francisco CA), Transgenic non-human animals capable of producing heterologous antibodies of various isotypes.
Goldenberg Milton D. (11837 Gainsborough Rd. Potomac MD 20854), Tumor localization and therapy with labeled antibody fragments specific to tumor-associated markers.
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