Methods of use of seed-origin endophyte populations
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A01N-063/00
A01N-063/02
A01C-001/06
A01C-001/00
출원번호
US-0826157
(2015-08-13)
등록번호
US-9295263
(2016-03-29)
발명자
/ 주소
von Maltzahn, Geoffrey
Flavell, Richard Bailey
Toledo, Gerardo V.
Djonovic, Slavica
Marquez, Luis Miguel
Johnston, David Morris
Millet, Yves Alain
Lyford, Jeffrey
Naydich, Alexander
Sadowski, Craig
출원인 / 주소
Symbiota, Inc.
대리인 / 주소
Fenwick & West LLP
인용정보
피인용 횟수 :
16인용 특허 :
29
초록▼
This application relates to methods and materials for providing a benefit to a seed or seedling of an agricultural plant (e.g., an agricultural grass plant), or the agricultural plant derived from the seed or seedling. For example, this application provides purified bacterial populations that includ
This application relates to methods and materials for providing a benefit to a seed or seedling of an agricultural plant (e.g., an agricultural grass plant), or the agricultural plant derived from the seed or seedling. For example, this application provides purified bacterial populations that include novel seed-origin bacterial endophytes, and synthetic combinations of seeds and/or seedlings (e.g., cereal seeds and/or seedlings) with heterologous seed-derived bacterial endophytes.
대표청구항▼
1. A method for increasing tolerance to drought of a cereal agricultural plant, said method comprising: (a) contacting seed of said plant with a composition comprising a purified bacterial population that comprises a first bacterial endophyte and a second bacterial endophyte, wherein the first bacte
1. A method for increasing tolerance to drought of a cereal agricultural plant, said method comprising: (a) contacting seed of said plant with a composition comprising a purified bacterial population that comprises a first bacterial endophyte and a second bacterial endophyte, wherein the first bacterial endophyte and second bacterial endophyte has a 16S nucleic acid sequence that is at least 97% identical to a 16S nucleic acid sequence selected from the group consisting of SEQ ID NO: 521, 533, 560, 588, 658, and 856, wherein the first bacterial endophyte and the second bacterial endophyte are of different genera and have different 16S nucleic acid sequence,(b) and growing said seed to produce plants colonized with the endophyte bacteria, wherein the plants have increased tolerance to drought stress as compared to a reference plant grown under the same conditions. 2. The method of claim 1, wherein the bacterial endophyte is obtainable from a different cultivar, variety or crop as compared to the seed. 3. The method of claim 1, wherein the bacterial endophyte colonizes the roots of the cereal agricultural plant. 4. The method of claim 1, wherein the composition further comprises a purified fungal population. 5. The method of claim 1, wherein the purified bacterial population comprises more than two bacterial endophytes. 6. The method of claim 1, wherein the bacterial endophyte is obtainable from an interior seed compartment. 7. The method of claim 1, wherein the bacterial endophyte is obtainable from an exterior surface of a seed. 8. The method of claim 1, wherein the bacterial endophyte is obtained from a rice seed. 9. The method of claim 1, wherein the bacterial endophyte is obtained from a maize seed. 10. The method of claim 1, wherein the bacterial endophyte is obtained from a wheat seed. 11. The method of claim 1, wherein the bacterial endophyte is obtained from a barley seed. 12. A method for increasing tolerance to oxidative stress of a cereal agricultural plant, said method comprising (a) contacting seed of said plant with a composition comprising a purified bacterial population that comprises a first bacterial endophyte and a second bacterial endophyte, wherein the first bacterial endophyte and second bacterial endophyte has a 16S nucleic acid sequence that is at least 97% identical to a 16S nucleic acid sequence selected from the group consisting of SEQ ID NO: 521, 533, 560, 588, 658, and 856, wherein the first bacterial endophyte and the second bacterial endophyte are of different genera and have different 16S nucleic acid sequence,(b) and growing said seed to produce plants colonized with the endophyte bacteria, wherein the plants have increased tolerance to oxidative stress as compared to a reference plant grown under the same conditions. 13. The method of claim 12, wherein the bacterial endophyte is obtainable from a different cultivar, variety or crop as compared to the seed. 14. The method of claim 12, wherein the bacterial endophyte colonizes the roots of the cereal agricultural plant. 15. The method of claim 12, wherein the composition further comprises a purified fungal population. 16. The method of claim 12, wherein the purified bacterial population comprises more than two bacterial endophytes. 17. The method of claim 12, wherein the bacterial endophyte is obtained from a rice seed. 18. The method of claim 1, wherein the bacterial endophyte is obtained from a maize seed. 19. The method of claim 1, wherein the bacterial endophyte is obtained from a wheat seed. 20. The method of claim 1, wherein the bacterial endophyte is obtained from a barley seed.
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von Maltzahn, Geoffrey; Flavell, Richard Bailey; Toledo, Gerardo V.; Djonovic, Slavica; Marquez, Luis Miguel; Johnston, David Morris; Millet, Yves Alain; Lyford, Jeffrey; Naydich, Alexander; Sadowski, Craig, Methods of use of seed-origin endophyte populations.
von Maltzahn, Geoffrey; Flavell, Richard Bailey; Toledo, Gerardo V.; Djonovic, Slavica; Marquez, Luis Miguel; Johnston, David Morris; Millet, Yves Alain; Lyford, Jeffrey; Naydich, Alexander; Sadowski, Craig, Methods of use of seed-origin endophyte populations.
von Maltzahn, Geoffrey; Flavell, Richard Bailey; Toledo, Gerardo V.; Djonovic, Slavica; Marquez, Luis Miguel; Johnston, David Morris; Millet, Yves Alain; Lyford, Jeffrey; Naydich, Alexander; Sadowski, Craig, Methods of use of seed-origin endophyte populations.
von Maltzahn, Geoffrey; Sadowski, Craig; Flavell, Richard Bailey; Toledo, Gerardo V.; Djonovic, Slavica; Marquez, Luis Miguel; Johnston, David Morris; Millet, Yves Alain; Lyford, Jeffrey; Naydich, Alexander, Methods of use of seed-origin endophyte populations.
Embree, Mallory, Methods, apparatuses, and systems for analyzing microorganism strains from complex heterogeneous communities, predicting and identifying functional relationships and interactions thereof, and selecting and synthesizing microbial ensembles based thereon.
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