Her Majesty the Queen in Right of Canada, as Represented by the Minister of Agriculture and Agri-Food
대리인 / 주소
Knobbe, Martens, Olson & Bear, LLP
인용정보
피인용 횟수 :
0인용 특허 :
15
초록▼
The invention provides methods of modifying the level of expression or functional activity of factors such as enzymes or other catalytic proteins or structural proteins, alone or in concert, to modify the frequency of meiotic homologous recombination involving the exchange of genetic information bet
The invention provides methods of modifying the level of expression or functional activity of factors such as enzymes or other catalytic proteins or structural proteins, alone or in concert, to modify the frequency of meiotic homologous recombination involving the exchange of genetic information between non-sister chromatids from homologous maternal and paternal chromosomes. The steps at which modulation may occur include: homologous chromosome pairing, double-strand break formation; resection; strand invasion; branch migration; and resolution. Methods of plant and animal breeding are also provided that utilize the modulation of meiotic homologous recombination.
대표청구항▼
1. A eukaryotic cell, wherein the cell is a yeast cell or a plant cell, the cell comprising a nucleic acid encoding a recombinant protein that is homologous to an endogenous protein of the yeast cell or the plant cell, wherein the recombinant protein is a DMC1 protein or a RAD51 protein, wherein the
1. A eukaryotic cell, wherein the cell is a yeast cell or a plant cell, the cell comprising a nucleic acid encoding a recombinant protein that is homologous to an endogenous protein of the yeast cell or the plant cell, wherein the recombinant protein is a DMC1 protein or a RAD51 protein, wherein the nucleic acid is operably linked to a promoter, wherein expression of the recombinant protein in the cell during meiosis increases the frequency of homologous non-sister chromatid exchange during meiosis compared to a eukaryotic cell that does not comprise the nucleic acid. 2. A eukaryotic cell, wherein the cell is a yeast cell or a plant cell, the cell comprising a nucleic acid encoding a recombinant protein that has a dominant negative effect on meiotic homologous recombination, wherein the recombinant protein is a heterologous DMC1 protein, a mutant DMC1 protein, a heterologous RAD51 protein, or a mutant RAD51 protein, wherein the nucleic acid is operably linked to a promoter, wherein expression of the recombinant protein in the cell during meiosis decreases the frequency of homologous non-sister chromatid exchange during meiosis compared to a eukaryotic cell that does not comprise the nucleic acid. 3. The eukaryotic cell of claim 2, wherein the mutant DMC1 protein includes an aspartate at a position corresponding to glycine 126 of Saccharomyces cerevisiae DMC1. 4. The eukaryotic cell of claim 2, wherein the mutant RAD51 protein includes an aspartate at a position corresponding to glycine 190 of Saccharomyces cerevisiae RAD51. 5. A method of increasing meiotic homologous recombination in a yeast or a plant, comprising: transforming the yeast cell or the plant cell with a nucleic acid encoding a recombinant protein that is homologous to an endogenous protein of the yeast cell or the plant cell, wherein the recombinant protein is a DMC1 protein or a RAD51 protein, wherein the nucleic acid is operably linked to a promoter, to form a transformed cell capable of expressing the recombinant protein during meiosis; andallowing the transformed cell, or a descendant of the transformed cell, to undergo meiosis to produce a viable gamete, wherein expression of the recombinant protein in the cell undergoing meiosis increases the frequency of homologous non-sister chromatid exchange during the meiosis. 6. The method of claim 5, wherein the promoter is inducible or repressible, wherein induction of the promoter increases expression of the nucleic acid, or wherein repression of the promoter inhibits expression of the nucleic acid. 7. The method of claim 5, wherein the gamete is crossed with a second gamete to obtain a progeny cell. 8. The method of claim 5, wherein the promoter is meiosis-specific. 9. A method of decreasing meiotic homologous recombination in a yeast or a plant, comprising: transforming the yeast cell or the plant cell with a nucleic acid encoding a recombinant protein that is a heterologous protein or a mutant protein that is homologous to an endogenous protein of the yeast cell or the plant cell, wherein the recombinant protein has a dominant negative effect on meiotic homologous recombination, wherein the recombinant protein is a heterologous DMC1 protein, a mutant DMC1 protein, a heterologous RAD51 protein or a mutant RAD51 protein, wherein the nucleic acid is operably linked to a promoter, to form a transformed cell capable of expressing the recombinant protein during meiosis; andallowing the transformed cell, or a descendant of the transformed cell, to undergo meiosis to produce a viable gamete, wherein expression of the recombinant protein in the cell undergoing meiosis decreases the frequency of homologous non-sister chromatid exchange during the meiosis. 10. The method of claim 9, wherein the mutant DMC1 protein includes an aspartate at a position corresponding to glycine 126 of Saccharomyces cerevisiae DMC1. 11. The method of claim 9, wherein the mutant RAD51 protein includes an aspartate at a position corresponding to glycine 190 of Saccharomyces cerevisiae RAD51. 12. The method of claim 9, wherein the transformed cell is a yeast cell and the heterologous protein is Arabidopsis thaliana DMC1 protein. 13. The method of claim 9, wherein the promoter is inducible or repressible, wherein induction of the promoter increases expression of the nucleic acid, or wherein repression of the promoter inhibits expression of the nucleic acid. 14. The method of claim 9, wherein the gamete is crossed with a second gamete to obtain a progeny cell. 15. The method of claim 9, wherein the promoter is meiosis-specific.
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이 특허에 인용된 특허 (15)
McCabe Dennis E. (Middleton WI) Martinell Brian J. (Madison WI), Apparatus for genetic transformation.
Doutriaux, Marie-Pascale; Betzner, Andreas Stefan; Freyssinet, Georges; Perez, Pascal, Isolated DNA that encodes an Arabidopsis thaliana MSH3 protein involved in DNA mismatch repair and a method of modifying the mismatch repair system in a plant transformed with the isolated DNA.
Fitzpatrick-McElligott Sandra G. (Media PA) Lavin John G. (Swarthmore PA) Rivard Germain F. (Philadelphia PA) Subramoney Shekhar (Hockessin DE), Method for introducing a biological substance into a target.
Sanford John C. (Geneva NY) Wolf Edward D. (Ithaca NY) Allen Nelson K. (Newfield NY), Method for transporting substances into living cells and tissues and apparatus therefor.
Hiatt William R. (Davis CA) Sheehy Raymond E. (Davis CA) Shewmaker Christine K. (Davis CA) Kridl Jean C. (Davis CA) Knauf Vic (Davis CA), PG gene and its use in plants.
Christou Paul (Madison WI) McCabe Dennis (Middleton WI) Swain William F. (Madison WI) Barton Kenneth A. (Middleton WI), Particle-mediated transformation of soybean plants and lines.
Schilperoort Robbert A. (Vincent van Goghlaan 40 2343 RP Oegstgeest NLX) Krens Frans A. (Scheveningen NLX) Wullems George J. (Warmond NLX), Process for the in-vitro transformation of plant protoplasts with plasmid DNA.
Schilperoort Robbert A. (Leiden NLX) Hoekema Andreas (Leiden NLX), Process for the incorporation of foreign DNA into the genome of dicotyledonous plants.
Schilperoort Robbert A. (Anthonie Duycklaan 10c ; 2334 CD Leiden NLX) Hoekema Andreas (Boerhaavelaan 114 ; 2334 ET Leiden NLX) Hooykaas Paul J. J. (Condorstraat 126 ; 2317 AW Leiden NLX), Process for the incorporation of foreign dna into the genome of dicotyledonous plants.
Paszkowski Jerzy (Riehen CHX) Potrykus Ingo (Magden CHX) Hohn Barbara (Bottmingen CHX) Shillito Raymond D. (Rheinfelden CHX) Hohn Thomas (Bottmingen CHX) Saul Michael W. (Binningen CHX) Mandak Vaclav, Transformation of hereditary material of plants.
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