System for fluid perfusion of biological matter comprising tissue
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A01N-001/00
A01N-001/02
C12M-001/36
C12M-001/38
C12M-003/00
C12M-001/00
출원번호
US-0341565
(2011-12-30)
등록번호
US-9357764
(2016-06-07)
발명자
/ 주소
Tempelman, Linda A.
Stone, Simon G.
출원인 / 주소
GINER, INC.
대리인 / 주소
Kriegsman & Kriegsman
인용정보
피인용 횟수 :
2인용 특허 :
22
초록▼
System for fluid perfusion of biological matter that includes tissue. According to one embodiment, the system may include a storage container for storing the biological matter, a thermal control device for cooling the contents of the storage container, a gas generator for generating a preserving gas
System for fluid perfusion of biological matter that includes tissue. According to one embodiment, the system may include a storage container for storing the biological matter, a thermal control device for cooling the contents of the storage container, a gas generator for generating a preserving gas, a fluid conduit coupled to the gas generator and insertable into tissue for delivering the preserving gas to the biological matter, and a process controller for controlling the operation of the gas generator and the thermal control device. The gas generator, in turn, may include an electrochemical oxygen concentrator and/or a water electrolyzer for generating the preserving gas. The system may further include a liquid perfusion system that includes a reservoir of liquid perfusate, a fluid delivery conduit for delivering liquid perfusate from the reservoir to the biological matter, and a fluid draining conduit for draining liquid perfusate from the biological matter.
대표청구항▼
1. A system for fluid perfusion of biological matter comprising tissue, the system comprising: (a) a gas persufflation system for use in persufflating the biological matter, wherein the gas persufflation system comprises a gas generator for producing a preserving gas;(b) a liquid perfusion system fo
1. A system for fluid perfusion of biological matter comprising tissue, the system comprising: (a) a gas persufflation system for use in persufflating the biological matter, wherein the gas persufflation system comprises a gas generator for producing a preserving gas;(b) a liquid perfusion system for use in perfusing the biological matter, the liquid perfusion system comprising a supply of liquid perfusate;(c) a delivery cannula, the delivery cannula having a first end and a second end, the first end being insertable into an inlet of the biological matter comprising tissue; and(d) a changeover system, the changeover system coupled to the second end of the delivery cannula and to each of the gas persufflation system and the liquid perfusion system, the changeover system comprising one or more valves configured to independently permit the preserving gas to flow from the gas persufflation system into the delivery cannula and the liquid perfusate to flow from the liquid perfusion system into the delivery cannula, whereby the preserving gas and the liquid perfusate may be selectively delivered to the biological matter comprising tissue in any of alternate, simultaneous, and sequential modes. 2. The system as claimed in claim 1 wherein the gas generator comprises a generator of gaseous oxygen. 3. The system as claimed in claim 2 wherein said gas generator comprises an electrolyzer. 4. The system as claimed in claim 2 wherein said gas generator comprises an electrochemical oxygen concentrator. 5. The system as claimed in claim 1 wherein the gas generator produces a mixture of gaseous oxygen and water vapor. 6. The system as claimed in claim 2 wherein the gas generator comprises an electrochemical oxygen generator, a first fluid line coupled to the electrochemical oxygen generator, a second fluid line coupled to ambient air, a third fluid line coupled to the first and second fluid lines, and an adjustable flow valve for adjusting the rate at which ambient air in the first fluid line is mixed with gaseous oxygen in the second fluid line so as to vary the concentration of gaseous oxygen in the preserving gas. 7. The system as claimed in claim 1 wherein the gas generator produces pure gaseous oxygen. 8. The system as claimed in claim 1 wherein said gas generator comprises at least one outlet for outputting a gas stream comprising the preserving gas. 9. The system as claimed in claim 8 wherein said gas generator comprises a plurality of outlets for outputting a corresponding plurality of gas streams comprising the preserving gas. 10. The system as claimed in claim 9 wherein said gas generator further comprises an adjustable flow valve coupled to each outlet so that each gas stream has an independently adjustable flow rate. 11. The system as claimed in claim 1 further comprising a container, the container being appropriately dimensioned to receive the biological matter comprising tissue. 12. The system as claimed in claim 11 wherein said container is a thermally insulated container. 13. The system as claimed 12 further comprising a temperature control system for maintaining the contents of said container at a desired temperature. 14. The system as claimed in claim 13 wherein said temperature control system comprises a temperature sensor for sensing the temperature within said container, a thermal control device for altering the temperature within said container, and a process controller, responsive to said temperature sensor, for controlling operation of said thermal control device. 15. The system as claimed in claim 1 wherein said liquid perfusion system further comprises a fluid draining conduit for draining liquid perfusate that has perfused through the biological matter comprising tissue. 16. The system as claimed in claim 1 wherein the liquid perfusate comprises a gas/liquid solution. 17. The system as claimed in claim 16 wherein the liquid is selected from the group consisting of a liquid cell culture medium, an organ preservation solution, a saline solution, a HEPES buffer, and combinations thereof. 18. The system as claimed in claim 17 wherein the liquid further comprises an additive selected from the group consisting of antioxidants, anti-apoptotic agents, vasodilators/vasoconstrictors, oxygen carriers, chelators, toxin binders, and anticoagulants. 19. A system for fluid perfusion of biological matter comprising tissue, the system comprising: (a) a storage container for storing the biological matter comprising tissue;(b) a thermal control system for maintaining the contents of the storage container at a desired temperature;(c) a gas persufflation system for persufflating the biological matter, wherein the gas persufflation system comprises a gas generator for producing a preserving gas;(d) a liquid perfusion system for perfusing the biological matter, the liquid perfusion system comprising a supply of liquid perfusate;(e) a delivery cannula, the delivery cannula having a first end and a second end, the first end being insertable into an inlet of the biological matter comprising tissue;(f) a first fluid assembly, the first fluid assembly comprising a first length of tubing, a first valve, and a second length of tubing, the first length of tubing being coupled at a first end to an outlet of the gas generator and at a second end to the first valve, the second length of tubing being coupled at a first end to the first valve and at a second end to the delivery cannula; and(g) a second fluid assembly, the second fluid assembly comprising a third length of tubing, a second valve, and a fourth length of tubing, the third length of tubing being coupled at a first end to an outlet of the supply of liquid perfusate and at a second end to the second valve, the fourth length of tubing being coupled at a first end to the second valve and at a second end to the delivery cannula;(h) wherein the first valve and the second valve are capable of being operated independently of one another, whereby the preserving gas and the liquid perfusate may be selectively delivered to the biological matter comprising tissue in any of simultaneous, alternative, and sequential modes. 20. The system as claimed in claim 19 wherein the gas generator produces gaseous oxygen. 21. The system as claimed in claim 20 wherein said gas generator comprises an electrochemical generator of gaseous oxygen. 22. The system as claimed in claim 21 wherein said electrochemical generator of gaseous oxygen comprises an electrolyzer. 23. The system as claimed in claim 21 wherein said electrochemical generator of gaseous oxygen comprises an electrochemical oxygen concentrator. 24. The system as claimed in claim 19 wherein the gas generator comprises an electrochemical oxygen generator, a first fluid line coupled to the electrochemical oxygen generator, a second fluid line coupled to ambient air, a third fluid line coupled to the first and second fluid lines, and an adjustable flow valve for adjusting the rate at which ambient air in the first fluid line is mixed with gaseous oxygen in the second fluid line so as to vary the concentration of gaseous oxygen in the preserving gas. 25. The system as claimed in claim 19 further comprising (h) an exit cannula, the exit cannula having a first end and a second end, the first end being insertable into an outlet of the biological matter comprising tissue;(i) a third fluid assembly, the third fluid assembly comprising a fifth length of tubing, a third valve, and a sixth length of tubing, the fifth length of tubing being coupled at a first end to the exit cannula and at a second end to the third valve, the sixth length of tubing being coupled at a first end to the third valve; and(j) a fourth fluid assembly, the fourth fluid assembly comprising a seventh length of tubing, a fourth valve, and an eighth length of tubing, the seventh length of tubing being coupled at a first end to the exit cannula and at a second end to the fourth valve, the eighth length of tubing being coupled at a first end to the fourth valve. 26. The system as claimed in claim 25 further comprising at least one gas sensor for monitoring a gas concentration in at least one of a fluid exiting the gas generator or the supply of liquid perfusate or a fluid entering or exiting the container. 27. A system for use in preservation of biological matter comprising tissue, said system comprising: (a) a storage container for storing the biological matter;(b) a temperature control device for maintaining the contents of the storage container at a desired temperature;(c) a gas persufflation system for use in persufflating the biological matter, wherein the gas persufflation system comprises a gas generator for generating a preserving gas;(d) a liquid perfusion system for use in perfusing the biological matter, the liquid perfusion system comprising a supply of liquid perfusate;(e) a changeover system fluidly coupling both the gas persufflation system and the liquid perfusion system to the storage container so as to control fluid communication between the gas persufflation system and storage container and between the liquid perfusion system and the storage container, the changeover system comprising one or more valves configured to independently permit the preserving gas to flow from the gas persufflation system into the storage container and the liquid perfusate to flow from the liquid perfusion system into the storage container, whereby the preserving gas and the liquid perfusate may be selectively delivered to the storage container in any of simultaneous, alternative, or sequential modes;(f) a process controller coupled to each of the temperature control device, the gas persufflation system, the liquid perfusion system, and the changeover system; and(g) a power supply. 28. The system as claimed in claim 27 wherein said gas generator comprises a plurality of outlets for outputting a corresponding plurality of gas streams and further comprises an adjustable flow valve coupled to each outlet so that each gas stream has an independently adjustable flow rate. 29. The system as claimed in claim 27 further comprising a fluid delivery tube and a fluid drainage tube. 30. The system as claimed in claim 27 wherein the changeover system comprises at least a first valve used to control fluid communication between the gas persufflation system and the storage container and at least a second valve used to control fluid communication between the fluid perfusion system and the storage container. 31. The system as claimed in claim 27 further comprising at least one oxygen gas sensor and/or at least one carbon dioxide sensor used to measure the concentrations of oxygen gas and/or carbon dioxide gas emitted from the gas persufflation system and/or the fluid perfusion system.
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이 특허에 인용된 특허 (22)
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Aebischer Patrick (Providence RI) Galletti Pierre M. (Providence RI) Panol George (Warwick RI) Miracoli Luigi (Genoa ITX), Implantable delivery system for biological factors.
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