초록

Methods of making tissue fillers are provided. In certain embodiments, the tissue is flake-like and has regenerative properties.

대표청구항 ▼

1. A method for preparing a tissue matrix composition comprising: selecting a collagen-based tissue matrix;contacting the tissue matrix with a cryoprotectant solution;freezing the tissue matrix and cryoprotective solution;cutting the frozen tissue matrix and cryoprotective solution into a plurality

1. A method for preparing a tissue matrix composition comprising: selecting a collagen-based tissue matrix;contacting the tissue matrix with a cryoprotectant solution;freezing the tissue matrix and cryoprotective solution;cutting the frozen tissue matrix and cryoprotective solution into a plurality of cut tissue pieces having irregular size and shape, wherein the temperature of the tissue matrix ranges from −10 ° C. to −40° C. for the cutting step and wherein the cut tissue has a size distribution ranging from 0.2-5 mm in length, 0.2-3 mm in width, and 0.02-0 3 mm in thickness;placing the plurality of cut tissue pieces into a liquid to form a suspension of interconnected irregularly shaped particles, andremoving liquid from the suspension to form a device having inter-connected channels that permit body fluid to flow freely therethrough. 2. The method of claim 1, wherein the tissue matrix comprises an acellular tissue matrix. 3. The method of claim 2, wherein the acellular tissue matrix comprises an acellular dermal matrix. 4. The method of claim 2, wherein the acellular tissue matrix comprises an acellular porcine dermal matrix. 5. The method of claim 1, wherein the cryoprotectant solution comprises at least one of a maltodextrin, sucrose, polyethylene glycol (PEG), or polyvinylpyrrolidone (PVP) solution, or a combination thereof. 6. The method of claim 1, wherein the cryoprotectant solution comprises a maltodextrin solution. 7. The method of claim 6, wherein the maltodextrin solution comprises 5-50% (w/v) maltodextrin. 8. The method of claim 6, wherein the maltodextrin solution comprises 15-25% (w/v) maltodextrin. 9. The method of claim 1, wherein cutting comprises grating. 10. The method of claim 1, wherein the tissue matrix is cut with a grater. 11. The method of claim 1, wherein the tissue matrix is cut with a grating wheel. 12. The method of claim 1, further comprising exposing the tissue matrix to a disinfectant. 13. The method of claim 12, wherein the disinfectant is isopropyl alcohol. 14. The method of claim 1, further comprising sterilizing the plurality of cut tissue pieces by application of at least one of ethylene oxide, propylene oxide, gamma irradiation or e-beam irradiation. 15. The method of claim 1, further comprising adding a bioactive substance to the cut matrix. 16. The method of claim 15, wherein the bioactive substance comprises an antimicrobial agent. 17. The method of claim 15, wherein the bioactive substance comprises a cytokine. 18. The method of claim 15, wherein the bioactive substance comprises a growth factor. 19. The method of claim 15, wherein the bioactive substance comprises non-collagenous tissue. 20. The method of claim 19, wherein the non-collagenous tissue comprises adipose tissue. 21. The method of claim 15, wherein the bioactive substance comprises cells. 22. The method of claim 21, wherein the cells comprise stem cells. 23. The method of claim 1, wherein removing liquid comprises freeze-drying the suspension.