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Locus specific amplification using array probes 원문보기

IPC분류정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판)
  • C40B-050/06
  • C12Q-001/68
출원번호 US-0211100 (2008-09-15)
등록번호 US-9388457 (2016-07-12)
발명자 / 주소
  • Fu, Glenn K.
  • Shapero, Michael H.
  • Wang, Pei-Hua
출원인 / 주소
  • Affymetrix, Inc.
대리인 / 주소
    Banner & Witcoff, Ltd.
인용정보 피인용 횟수 : 0  인용 특허 : 146

초록

Methods are provided for multiplexed amplification of selected targets and analysis of the amplified targets. In preferred aspects the amplification and analysis take place on the same solid support and preferably in a localized area such as a bead or a feature of an array. In preferred aspects the

대표청구항

1. A method for amplifying a plurality of target nucleic acid sequences from an un-amplified nucleic acid sample and analyzing the amplified target sequences, said method comprising: (a) fragmenting the un-amplified nucleic acid sample to obtain un-amplified target nucleic acid fragments;(b) mixing

이 특허에 인용된 특허 (146)

  1. Hunkapiller Michael W. ; Richards John H., Adapter directed expression analysis.
  2. DuBridge Robert B. ; Albrecht Glenn ; Brenner Sydney,GBX ; Gryaznov Sergei M. ; McCurdy Sarah N., Adaptor-based sequence analysis.
  3. Kurn,Nurith, Amplification of RNA sequences using composite RNA-DNA primers and strand displacement.
  4. Fry Kirk ; Larrick James ; Tam Albert, Amplification of mixed sequence nucleic acid fragments.
  5. Rothberg Jonathan Marc ; Deem Michael W. ; Simpson John W., Apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing.
  6. McClelland Michael (Del Mar CA) Welsh John T. (Leucadia CA) Sorge Joseph A. (Rancho Santa Fe CA), Arbitrarily primed polymerase chain reaction method for fingerprinting genomes.
  7. Beattie Kenneth Loren, Arbitrary sequence oligonucleotide fingerprinting.
  8. Chee Mark ; Cronin Maureen T. ; Fodor Stephen P. A. ; Huang Xiaohua X. ; Hubbell Earl A. ; Lipshutz Robert J. ; Lobban Peter E. ; Morris MacDonald S. ; Sheldon Edward L., Arrays of nucleic acid probes on biological chips.
  9. Sapolsky Ronald J. ; Lipshutz Robert J. ; Gingeras Thomas R., Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping.
  10. Wolber, Paul K.; Kincaid, Robert H.; Amorese, Douglas; Ilsley Tyree, Diane; Atwell, Andrew S.; Kronick, Mel N.; Leproust, Eric M., Chemical arrays and methods of using the same.
  11. Kaiser Michael W. ; Lyamichev Victor I. ; Lyamicheva Natasha, Cleavage agents.
  12. Prudent James R. ; Hall Jeff G. ; Lyamichev Victor I. ; Brow Mary Ann D. ; Dahlberg James E., Cleavage of nucleic acids.
  13. Ausubel Frederick ; Davis Ronald W. ; Preuss Daphne, Cleaved amplified RFLP detection methods.
  14. Dong,Shoulian; Lipshutz,Robert J.; Lockhart,David, Complexity management and analysis of genomic DNA.
  15. Shoulian Dong ; Robert J. Lipshutz ; David J. Lockhart, Complexity management and analysis of genomic DNA.
  16. Dong, Shoulian, Complexity management of genomic DNA.
  17. Dong, Shoulian, Complexity management of genomic DNA.
  18. Su, Xing, Complexity management of genomic DNA.
  19. Jones,Keith W.; Shapero,Michael; Liu,Weiwei, Complexity management of genomic DNA by locus specific amplification.
  20. Makarov Vladimir L. ; Langmore John P., Compositions and methods for analysis of nucleic acids.
  21. Makarov, Vladimir L.; Langmore, John P., Compositions and methods for analysis of nucleic acids.
  22. Reyes Gregory R. ; Kim Jungsuh, DNA amplification and subtraction techniques.
  23. Caskey Charles T. (Houston TX) Edwards Albert O. (Houston TX), DNA typing with short tandem repeat polymorphisms and identification of polymorphic short tandem repeats.
  24. Babon Jeff,AUX ; Youil Rima,AUX ; Stoerker Jay ; Huff Anne ; Cotton Richard G. H.,AUX, Detection of mismatches by resolvase cleavage using a magnetic bead support.
  25. Mark S. Chee ; Kevin Gunderson, Detection of nucleic acid amplification reactions using bead arrays.
  26. Troubounis, George; Menke, Lucas; Wünsche, Gisbert, Device for separating fluids.
  27. Willis, Thomas D.; Hardenbol, Paul; Jain, Maneesh; Stolc, Viktor; Ronaghi, Mostafa; Davis, Ronald W., Direct multiplex characterization of genomic DNA.
  28. Short Jay M. ; Frey Gerhard Johann, End selection in directed evolution.
  29. Okamoto Naoaki (South Setauket NY), Enzyme digestion method for the detection of amplified DNA.
  30. Lockhart David J. ; Brown Eugene L. ; Wong Gordon G. ; Chee Mark S. ; Gingeras Thomas R., Expression monitoring by hybridization to high density oligonucleotide arrays.
  31. Erlich Henry A. (Oakland CA), HLA typing method and cDNA probes used therein.
  32. Felder Stephen ; Kris Richard M., High throughput assay system.
  33. Hillman Jennifer L. ; Shah Purvi ; Corley Neil C., Human peroxisomal thioesterase.
  34. Deugau Kenneth V. (Kingston CAX) Unrau Paul (Deep River CAX), Indexing linkers.
  35. Deugau Kenneth Victor,CAX ; Unrau Paul,CAX, Indexing linkers.
  36. Munroe David J. (Somerville MA) Housman David E. (Newton MA), Interspersed repetitive element-bubble amplification of nucleic acids.
  37. Keith Douglas H. (Oakland CA) Kronick Mel N. (Palo Alto CA) McBride Lincoln J. (Redwood City CA) Whiteley Norman M. (San Carlos CA), Labeling by simultaneous ligation and restriction.
  38. Pirrung Michael C. (Durham NC) Read J. Leighton (Palo Alto CA) Fodor Stephen P. A. (Palo Alto CA) Stryer Lubert (Stanford CA), Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof.
  39. Backman Keith C. (Bedford MA) Carrino John J. (Gurnee IL) Shimer George H. (Boston MA) Yocum Robert R. (Lexington MA), Ligase chain reaction with endonuclease IV correction and contamination control.
  40. Albrecht Glenn ; Brenner Sydney,GBX ; DuBridge Robert B. ; Lloyd David H. ; Pallas Michael C., Massively parallel signature sequencing by ligation of encoded adaptors.
  41. Macevicz, Stephen C., Method and compositions for ordering restriction fragments.
  42. Backman Keith C. ; Bond Sheila B. ; Carrino John J. ; Laffler Thomas G., Method and kits for amplifying target nucleic acids applicable to both polymerase and ligase chain reactions.
  43. Granados Edward N. ; Bouma Stanley R. ; Carrino John J. ; Solomon Natalie A., Method and reagent for detecting multiple nucleic acid sequences in a test sample.
  44. Mukai, Hiroyuki; Sagawa, Hiroaki; Uemori, Takashi; Yamamoto, Junko; Tomono, Jun; Kobayashi, Eiji; Enoki, Tatsuji; Takeda, Osamu; Miyake, Kazue; Sato, Yoshimi; Moriyama, Mariko; Sawaragi, Haruhisa; Ha, Method for amplifying nucleic acid sequence.
  45. Cleuziat Philippe,FRX ; Mandrand Bernard,FRX, Method for amplifying nucleic acid sequences by strand displacement using DNA/RNA chimeric primers.
  46. Saiki Randall K. (Richmond CA) Erlich Henry A. (Oakland CA), Method for detection of polymorphic restriction sites and nucleic acid sequences.
  47. Shannon Karen W., Method for linear mRNA amplification.
  48. 100 ; Takagaki et al., Method for performing amplification of nucleic acid on supports.
  49. Adams Christopher P. (Winter Hill MA) Kron Stephen Joseph (Boston MA), Method for performing amplification of nucleic acid with two primers bound to a single solid support.
  50. Richards Rodney M. ; Jones Theodore ; Snitman David L. ; Brown Gregory S., Method for reducing carryover contamination in an amplification procedure.
  51. Chenchik Alex (Palo Alto CA) Diatchenko Luda (Palo Alto CA) Siebert Paul (Sunnyvale CA) Lukianov Sergey (Moscow SUX) Lukianov Konstantin (Moscow SUX) Gurskaya Nadia (Moscow SUX) Tarabykin Victor (Mos, Method for suppressing DNA fragment amplification during PCR.
  52. Chenchik Alex ; Diatchenko Luda ; Siebert Paul ; Lukianov Sergey,RUX ; Lukianov Konstantin,RUX ; Gurskaya Nadia,RUX ; Tarabykin Victor,RUX ; Sverdlov Eugene,RUX, Method for suppressing DNA fragment amplification during PCR.
  53. Drmanac Radoje T. (Beograd YUX) Crkvenjakov Radomir B. (Beograd YUX), Method of determining an ordered sequence of subfragments of a nucleic acid fragment by hybridization of oligonucleotide.
  54. Livak Kenneth J. (Wilmington DE) Brenner Sydney (Cambridge GB2), Method of gene mapping.
  55. Larry G. Birkenmeyer ; Thomas P. Leary ; A. Scott Muerhoff ; Suresh M. Desai ; Isa K. Mushahwar, Method of performing subtractive hybridization using RDA.
  56. Zajaczkowski Michael J. (Yoe PA) Stutzman Barbara A. (Dover PA), Method of preparation of water-soluble copolymer.
  57. Eyal Nurit,ILX ; Navot Nir,ILX, Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits t.
  58. Nyren Pal,SEX, Method of sequencing DNA based on the detection of the release of pyrophosphate and enzymatic nucleotide degradation.
  59. Drmanac Radoje T. (Beograd YUX) Crkvenjakov Radomir B. (Beograd YUX), Method of sequencing by hybridization of oligonucleotide probes.
  60. Drmanac Radoje T. (Zvecanska 46 Beograd 11000) Crkvenjakov Radomir B. (Bulevar JNA 118 Beograd YUX 11000), Method of sequencing of genomes by hybridization of oligonucleotide probes.
  61. Drmanac Radoje T. (Beograd YUX) Crkvenjakov Radomir B. (Beograd YUX), Method of sequencing of genoms by hybridization of oligonucleotide probes.
  62. Chetverin Alexander B.,RUX ; Kramer Fred Russell, Method of sorting a mixture of nucleic acid strands on a binary array.
  63. Su, Xing; Dong, Shoulian, Method of target enrichment and amplification.
  64. Su,Xing; Dong,Shoulian, Method of target enrichment and amplification.
  65. Mark Chee, Method to detect gene polymorphisms and monitor allelic expression employing a probe array.
  66. Glahn et al., Methods and adaptors for generating specific nucleic acid populations.
  67. Kurn, Nurith, Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof.
  68. Kurn, Nurith, Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof.
  69. Kurn, Nurith, Methods and compositions for linear isothermal amplification of polynucleotide sequences.
  70. Kurn Nurith, Methods and compositions for linear isothermal amplification of polynucleotide sequences, using a RNA-DNA composite primer.
  71. Kurn, Nurith, Methods and compositions for transcription-based nucleic acid amplification.
  72. Landers, John; Jordan, Barbara; Housman, David E.; Charest, Alain, Methods and products related to genotyping and DNA analysis.
  73. Sapolsky, Ronald J.; Lipshutz, Robert J.; Gingeras, Thomas R., Methods for analyzing nucleic acids using a type IIs restriction endonuclease.
  74. Brown Patrick O. ; Shalon Tidhar Dari, Methods for fabricating microarrays of biological samples.
  75. Shapero,Michael H.; Jones,Keith W., Methods for genotyping with selective adaptor ligation.
  76. Glahn et al., Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom.
  77. Schafer Alan J.,GBX ; Foster Jamie W.,GBX, Methods for obtaining nucleic acid containing a mutation.
  78. Wigler Michael (Lloyd Harbor NY) Lisitsyn Nikolai (Cold Spring Harbor NY), Methods for producing probes capable of distinguishing DNA from related sources.
  79. Weissman Sherman ; Lasken Roger ; Pan Xinghua, Methods for reducing the complexity of DNA sequences.
  80. Porat,Noga; Porat,Boaz, Methods for variation detection.
  81. Smith Craig E. ; York Charles K., Methods of isolating biological target materials using silica magnetic particles.
  82. Adessi,Celine; Kawashima,Eric; Mayer,Pascal; Mermod,Jean Jacques; Turcatti,Gerardo, Methods of nucleic acid amplification and sequencing.
  83. Gentalen Erik ; Chee Mark, Methods of using an array of pooled probes in genetic analysis.
  84. Perrin, Steven; Call, Kathy; Connolly, Timothy, Microarray-based subtractive hybridzation.
  85. Vinayagamoorthy Thuraiayah,CAX, Multi-loci genomic analysis.
  86. Urdea Michael S. (Alamo CA) Horn Thomas (Berkeley CA), Multifunctional nucleic acid monomer.
  87. Faham,Malek; Namsaraev,Eugeni Anatolyevich; Willis,Thomas Daniel, Multiplex PCR.
  88. Caskey Charles T. (Houston TX) Chamberlain Jeffrey S. (Houston TX) Gibbs Richard A. L. (Houston TX) Ranier Joel E. (Houston TX) Nguyen Phi N. (Houston TX), Multiplex genomic DNA amplification for deletion detection.
  89. Faham, Malek; Zheng, Jianbiao, Multiplex oligonucleotide addition and target amplification.
  90. Faham,Malek; Zheng,Jianbiao, Multiplex oligonucleotide addition and target amplification.
  91. Lipshutz Robert J. ; Morris MacDonald S. ; Chee Mark S. ; Gingeras Thomas R., Nucleic acid affinity columns.
  92. Lipshutz Robert J. ; Morris MacDonald S. ; Chee Mark S. ; Gingeras Thomas R., Nucleic acid affinity columns.
  93. Rose Samuel (3401 Hillview Ave. ; P.O. Box 10850 Palo Alto CA 94303) Goodman Thomas C. (3401 Hillview Ave. ; P.O. Box 10850 Palo Alto CA 94303) Western Linda M. (3401 Hillview Ave. ; P.O. Box 10850 P, Nucleic acid amplification using single primer.
  94. Rose Samuel ; Goodman Thomas C. ; Western Linda M. ; Becker Martin ; Ullman Edwin F., Nucleic acid amplification using single primer.
  95. Fan, Jian-Bing; Fu, Xiang-Dong, Nucleic acid detection methods using universal priming.
  96. Guilfoyle Richard A. ; Guo Zhen, Nucleic acid indexing.
  97. Kacian Daniel L. (San Diego CA) McAllister Diane L. (San Diego CA) McDonough Sherrol H. (San Diego CA) Dattagupta Nanibhushan (San Diego CA), Nucleic acid sequence amplification method, composition and kit.
  98. Macevicz Stephen C. (21890 Rucker Dr. Cupertino CA 95014), Nucleic acid sequence determination by multiple mixed oligonucleotide probes.
  99. Chetverin Alexander B.,RUX ; Kramer Fred Russell, Oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids.
  100. Monforte Joseph Albert (Berkeley CA) Becker Christopher Hank (Menlo Park CA) Shaler Thomas Andrew (San Francisco CA) Pollart Daniel Joseph (Menlo Park CA), Oligonucleotide sizing using immobilized cleavable primers.
  101. Hornes Erik (Oslo NOX) Korsnes Lars (Oslo NOX), Oligonucleotide-linked magnetic particles and uses thereof.
  102. Urdea Michael S. (Alamo CA) Horn Thomas (Berkeley CA), Oligonucleotides with cleavable sites.
  103. Urdea Michael S. (Alamo CA) Horn Thomas (Berkeley CA), Oligonucleotides with selectably cleavable and/or abasic sites.
  104. Blanco Luis (Madrid) Bernad Antonio (Madrid) Salas Margarita (Madrid ESX), PHI29 DNA polymerase.
  105. Wong Wing H., Parallel polynucleotide sequencing method using tagged primers.
  106. Church,George M.; Shendure,Jay; Porreca,Gregory J.; Zhu,Jun, Polony fluorescent in situ sequencing beads.
  107. Lipshutz Robert J. ; Sapolsky Ronald ; Ghandour Ghassan, Polymorphism detection.
  108. Wu Annie L. (Penfield NY), Preparation and isolation of single-stranded biotinylated nucleic acids by heat avidin-biotin cleavage.
  109. Hacia Joseph G. ; Chee Mark S. ; Collins Francis S., Probe-based analysis of heterozygous mutations using two-color labelling.
  110. Van Gelder Russell N. (2200 Divot Dr. Crystal Lake MD 63131) Von Zastrow Mark E. (11570 Buena Vista Dr. Los Altos CA 94022) Barchas Jack D. (1900 Rittenhouse Sq. #18A Philadelphia PA 19103) Eberwine , Process for amplifying a target polynucleotide sequence using a single primer-promoter complex.
  111. Mullis Kary B. (Kensington CA), Process for amplifying nucleic acid sequences.
  112. Mullis Kary B. (La Jolla CA) Erlich Henry A. (Oakland CA) Arnheim Norman (Woodland Hills CA) Horn Glenn T. (Emeryville CA) Saiki Randall K. (Richmond CA) Scharf Stephen J. (Berkeley CA), Process for amplifying, detecting, and/or cloning nucleic acid sequences.
  113. Mullis Kary B. (Kensington CA) Erlich Henry A. (Oakland CA) Arnheim Norman (Woodland Hills CA) Horn Glenn T. (Emeryville CA) Saiki Randall K. (Richmond CA) Scharf Stephen J. (Berkeley CA), Process for amplifying, detecting, and/or-cloning nucleic acid sequences.
  114. Hartley James L. (Frederick MD) Berninger Mark (Gaithersburg MD), Process for controlling contamination of nucleic acid amplification reactions.
  115. Hartley, James L., Process for controlling contamination of nucleic acid amplification reactions.
  116. Subrahmanyam V. Yerramilli ; Yatindra Prashar ; Peter Newburger ; Jon Goguen ; Sherman M. Weissman, Process to study changes in gene expression in granulocytic cells.
  117. Van Gelder Russell N. ; Von Zastrow Mark E. ; Barchas Jack D. ; Eberwine James H., Processes for genetic manipulations using promoters.
  118. Lopez-Nieto Carlos Eduardo ; Nigam Sanjay Kumar, Processes, apparatus and compositions for characterizing nucleotide sequences based on K-tuple analysis.
  119. Short Jay M. ; Mathur Eric J., Production and use of normalized DNA libraries.
  120. Short Jay M. ; Mathur Eric J., Production and use of normalized DNA libraries.
  121. Wang Bruce, Quantitative microarray hybridizaton assays.
  122. S철derlund,Hans E.; Syvanen,Anne Christine, Reagent kit for determining specific nucleotide variations.
  123. Guire, Patrick E.; Swanson, Melvin J., Replicable probe array.
  124. Wigler Michael ; Lisitsyn Nikolai, Representational approach to DNA analysis.
  125. Wigler Michael ; Lisitsyn Nikolai, Representational approach to DNA analysis.
  126. George ; Jr. Albert L. (Gaithersburg MD), Restriction amplification assay.
  127. George ; Jr. Albert L. (Gaithersburg MD), Restriction amplification assay.
  128. Hunkapiller Michael W. ; Richards John H., Restriction enzyme mediated adapter.
  129. Keith Douglas H. (Oakland CA), Restriction/ligation labeling for primer initiated multiple copying of DNA ssequences.
  130. Lizardi Paul M.,MXX, Rolling circle replication reporter systems.
  131. Paul M. Lizardi MX, Rolling circle replication reporter systems.
  132. Zabeau Marc,BEX ; Vos Pieter,NLX, Selective restriction fragment amplification: fingerprinting.
  133. Dower William J. (Menlo Park CA) Fodor Stephen P. A. (Palo Alto CA), Sequencing of surface immobilized polymers utilizing microflourescence detection.
  134. Walker George T. (Chapel Hill NC) Nadeau James G. (Chapel Hill NC) Little Michael C. (Raleigh NC), Simultaneous amplification of multiple targets.
  135. Pedersen, Morten Lorentz, Single-stranded polynucleotide tags.
  136. Boles T. Christian ; Abrams Ezra S., Solid phase methods for amplifying multiple nucleic acids.
  137. Weinshilboum Richard M. (Rochester MN) Honchel Ronald (Kasson MN) Aksoy Ibrahim A. (Rochester MN) Szumlanski Carol L. (Rochester MN) Wood Thomas C. (Rochester MN) Otterness Diane M. (Rochester MN) Wi, Stably-transformed cells expressing human thiopurine methyltransferase.
  138. Walker George T. (Chapel Hill NC), Strand displacement amplification.
  139. Fraiser Melinda S. (Durham NC) Spargo Catherine A. (Cary NC) Walker George Terrance (Chapel Hill NC) Van Cleve Mark (San Jose CA) Wright David James (Chapel Hill NC) Little Michael C. (Baltimore MD), Strand displacement amplification using thermophilic enzymes.
  140. Fraiser Melinda S. ; Spargo Catherine A. ; Walker George Terrance ; Van Cleve Mark ; Wright David James ; Little Michael C., Strand displacement amplification using thermophilic enzymes.
  141. Malek Lawrence T.,CAX ; Sooknanan Roy R.,CAX, Subtractive amplification.
  142. Koepsell Hermann,DEX ; Grundemann Dirk,DEX ; Gorboulev Valentin,DEX, Transport protein which effects the transport of cationic xenobiotics and/or pharmaceuticals, DNA sequences encoding it and their use.
  143. Lizardi Paul M.,MXX ; Caplan Michael, Unimolecular segment amplification and sequencing.
  144. Wagner ; Jr. Robert E., Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, purification of amplified DNA samples and allele identification.
  145. McCasky Feazel Rhonda J. ; Helentjaris Timothy G. ; Malmberg Sharon E. ; Martin Barry A., Use of selective DNA fragment amplification products for hybridization-based genetic fingerprinting, marker assisted selection, and high-throughput screening.
  146. Rhonda J. McCasky Feazel ; Timothy G. Helentjaris ; Sharon E. Malmberg ; Barry A. Martin, Use of selective DNA fragment amplification products for hybridization-based genetic fingerprinting, marker assisted selection, and high-throughput screening.
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