[미국특허]
Polynucleotides, polypeptides and methods for increasing oil content, growth rate and biomass of plants
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12N-015/82
C07K-014/415
출원번호
US-0936226
(2013-07-08)
등록번호
US-9487793
(2016-11-08)
발명자
/ 주소
Emmanuel, Eyal
Ronen, Gil
Savir, Noa
출원인 / 주소
Evogene Ltd.
인용정보
피인용 횟수 :
0인용 특허 :
57
초록▼
Provided are method of increasing oil content, growth rate, biomass, yield and/or vigor of a plant. The methods are effected by upregulating in the plant an expression level of a polypeptide comprising an amino acid sequence at least 90% homologous to the amino acid sequence selected from the group
Provided are method of increasing oil content, growth rate, biomass, yield and/or vigor of a plant. The methods are effected by upregulating in the plant an expression level of a polypeptide comprising an amino acid sequence at least 90% homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs: 199, 166-198, 200-221, 229-307, 311-330, 351-353, 355-361, 363-364, 366-368, 218, 222-228, 308-310, 350, 354, 362, 365, 523-649, 786-920, 1047 and 1048. Also provided are polynucleotides, nucleic acid constructs, polypeptides and transgenic plants expressing same which can be used to increase oil content, growth rate, biomass, yield and/or vigor of a plant and produce oil.
대표청구항▼
1. A method of increasing oil content and/or seed yield of a plant, comprising: (a) introducing into the plant an exogenous polynucleotide encoding the polypeptide set forth by SEQ ID NO: 329 or a homologous polypeptide which comprises conservative amino acid substitution(s) with respect to the amin
1. A method of increasing oil content and/or seed yield of a plant, comprising: (a) introducing into the plant an exogenous polynucleotide encoding the polypeptide set forth by SEQ ID NO: 329 or a homologous polypeptide which comprises conservative amino acid substitution(s) with respect to the amino acid sequence set forth by SEQ ID NO: 329, and which exhibits at least 93% sequence identity to the amino acid sequence set forth by SEQ ID NO: 329, and;(b) selecting plants resultant of step (a) for increased oil content and/or increased seed yield as compared to a non-transformed plant of the same species which is grown under the same growth conditions,thereby increasing the oil content and/or the seed yield of the plant. 2. A method of producing oil, comprising: (a) providing the plant according to claim 1; and(b) extracting the oil from the plant;thereby producing the oil. 3. The method of claim 1, wherein said exogenous polynucleotide comprises the nucleic acid sequence set forth by SEQ ID NO: 164 or a codon optimized nucleic acid sequence thereof. 4. The method of claim 1, wherein said amino acid sequence is selected from the group consisting of SEQ ID NOs: 329, 912, 914 and 916. 5. The method of claim 1, wherein said polynucleotide is selected from the group consisting of SEQ ID NOs: 164, 777, 781, and 779. 6. The method of claim 1, wherein said polypeptide is set forth by SEQ ID NO: 329. 7. The method of claim 1, wherein the oil comprises a seed oil. 8. The method of claim 1, wherein said polynucleotide comprises the nucleic acid sequence set forth by SEQ ID NO: 164. 9. A method of selecting a transformed plant having increased oil content and/or increased seed yield as compared to a non-transformed plant, the method comprising: (a) providing plants transformed with an exogenous polynucleotide encoding the polypeptide set forth by SEQ ID NO: 329 or a homologous polypeptide which comprises conservative amino acid substitution(s) with respect to the amino acid sequence set forth by SEQ ID NO: 329, and which exhibits at least 93% sequence identity to the amino acid sequence set forth by SEQ ID NO: 329,(b) selecting from said plants of step (a) a plant having increased oil content and/or increased seed yield as compared to a non-transformed plant of the same species which is grown under the same growth conditions,thereby selecting the plant having the increased oil content and/or the increased seed yield as compared to the non-transformed plant. 10. The method of claim 9, wherein said amino acid sequence is selected from the group consisting of SEQ ID NOs: 329, 912, 914 and 916. 11. The method of claim 9, wherein said exogenous polynucleotide encodes the polypeptide set forth by SEQ ID NO: 329. 12. The method of claim 9, wherein said exogenous polynucleotide comprises the nucleic acid sequence set forth by SEQ ID NO: 164 or a codon optimized sequence thereof. 13. The method of claim 9, wherein said exogenous polynucleotide comprises the nucleic acid sequence set forth by SEQ ID NO: 164. 14. A method of increasing oil content and/or seed yield of a plant, comprising: (a) introducing into the plant an exogenous polynucleotide encoding a polypeptide comprising the amino acid sequence set forth by SEQ ID NO: 329, and;(b) selecting plants resultant of step (a) for increased oil content and/or increased seed yield as compared to a non-transformed plant of the same species which is grown under the same growth conditions,thereby increasing the oil content and/or the seed yield of the plant. 15. A method of producing oil, comprising: (a) providing the plant according to claim 14; and(b) extracting the oil from the plant;thereby producing the oil. 16. A method of selecting a transformed plant having increased oil content and/or increased seed yield as compared to a non-transformed plant, the method comprising: (a) providing plants transformed with an exogenous polynucleotide encoding a polypeptide comprising the amino acid sequence set forth by SEQ ID NO: 329,(b) selecting from said plants of step (a) a plant having increased oil content and/or increased seed yield as compared to a non-transformed plant of the same species which is grown under the same growth conditions,thereby selecting the plant having the increased oil content and/or increased seed yield as compared to the non-transformed plant.
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