[미국특허]
Polynucleotides, polypeptides encoded thereby, and methods of using same for increasing abiotic stress tolerance and/or biomass and/or yield in plants expressing same
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A01H-005/10
A01H-001/04
C12N-015/82
C07K-014/415
출원번호
US-0071715
(2013-11-05)
등록번호
US-9518267
(2016-12-13)
발명자
/ 주소
Ronen, Gil
Karchi, Hagai
Diber, Alex
Vinocur, Basia Judith
Ayal, Sharon
Emmanuel, Eyal
Gang, Michael
Dimet, Dotan
출원인 / 주소
Evogene Ltd.
인용정보
피인용 횟수 :
0인용 특허 :
57
초록▼
Provided are methods of increasing tolerance of a plant to abiotic stress, and/or increasing biomass, growth rate, vigor and/or yield of a plant. The methods are effected by expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising an amino acid sequence at least 90%
Provided are methods of increasing tolerance of a plant to abiotic stress, and/or increasing biomass, growth rate, vigor and/or yield of a plant. The methods are effected by expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising an amino acid sequence at least 90% homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs:201, 207, 212, 202-206, 208-211, 213-391, 1655, 961-1529, and 1660-1663. Also provided are polynucleotides, nucleic acid constructs, polypeptides and transgenic plants expressing same which can be used to increase tolerance of a plant to abiotic stress, and/or increase biomass, growth rate, vigor and/or yield of a plant.
대표청구항▼
1. A method of increasing biomass, growth rate, seed yield, and/or abiotic stress tolerance of a plant as compared to the biomass, growth rate, seed yield, and/or abiotic stress tolerance of a control plant of the same species which is grown under the same growth conditions, wherein the abiotic stre
1. A method of increasing biomass, growth rate, seed yield, and/or abiotic stress tolerance of a plant as compared to the biomass, growth rate, seed yield, and/or abiotic stress tolerance of a control plant of the same species which is grown under the same growth conditions, wherein the abiotic stress is selected from the group consisting of osmotic stress, salinity stress and nutrient deficiency, the method comprising: (a) overexpressing within the plant an exogenous polynucleotide encoding a polypeptide comprising the amino acid sequence set forth by SEQ ID NO:201 wherein said polypeptide is capable of increasing biomass, growth rate, seed yield and/or abiotic stress tolerance of a plant, wherein the abiotic stress is selected from the group consisting of osmotic stress, salinity stress and nutrient deficiency, wherein said exogenous polynucleotide is comprised in a nucleic acid construct which further comprises —a promoter for directing transcription of said exogenous polynucleotide in a plant cell, said promoter is —a constitutive promoter, a tissue-specific, or an abiotic stress-inducible promoter, and;(b) selecting from plants resultant of step (a) a plant exhibiting an increased biomass, growth rate, seed yield, and/or abiotic stress tolerance as compared to a control plant of the same species which is grown under the same growth conditions, wherein the abiotic stress is selected from the group consisting of osmotic stress, salinity stress and nutrient deficiency,thereby increasing the biomass, growth rate, seed yield, and/or abiotic stress tolerance of the plant as compared to the biomass, growth rate, seed yield, and/or abiotic stress tolerance of the control plant of the same species which is grown under the same growth conditions. 2. The method of claim 1, wherein said promoter is a constitutive promoter. 3. The method of claim 1, wherein said exogenous polynucleotide is set forth in SEQ ID NO:1530, 1, or 1531. 4. The method of claim 1, wherein said polynucleotide is heterologous to said plant. 5. The method of claim 1, further comprising growing the plant expressing said exogenous polynucleotide under the abiotic stress. 6. The method of claim 1, wherein said nutrient deficiency comprises nitrogen deficiency. 7. A method of selecting a plant having increased biomass, growth rate, seed yield, and/or abiotic stress tolerance as compared to a control plant of the same species which is grown under the same growth conditions, the method comprising: (a) providing plants transformed with an exogenous polynucleotide encoding a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:201,(b) selecting from said plants a plant having increased biomass, growth rate, seed yield, and/or abiotic stress tolerance, wherein said abiotic stress is selected from the group consisting of osmotic stress, salinity stress and nutrient deficiency,thereby selecting the plant having increased biomass, growth rate, seed yield, and/or abiotic stress tolerance as compared to the control plant of the same species which is grown under the same growth conditions. 8. The method of claim 7, further comprising: (c) growing a crop of said plant transformed with said exogenous polynucleotide. 9. The method of claim 8, wherein said growing comprises seeding seeds and/or planting plantlets of said plant transformed with said exogenous polynucleotide. 10. The method of claim 7, wherein said nutrient deficiency comprises nitrogen deficiency. 11. The method of claim 7, wherein said exogenous polynucleotide is set forth in SEQ ID NO:1530, 1, or 1531.
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