[특허]Structure based and combinatorially selected oligonucleoside phosphorothioate and phosphorodithioate aptamer targeting AP-1 transcription factors

Structure based and combinatorially selected oligonucleoside phosphorothioate and phosphorodithioate aptamer targeting AP-1 transcription factors 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	G01N-033/68 C12N-015/10
출원번호	US-0014597 (2011-01-26)
등록번호	US-9567579 (2017-02-14)
발명자 / 주소	Gorenstein, David G. Luxon, Bruce A. Leary, James Yang, Xianbin
출원인 / 주소	Board of Regents, The University of Texas System
대리인 / 주소	Flores, Edwin S.
인용정보	피인용 횟수 : 0 인용 특허 : 74

초록 ▼

The present invention includes composition and methods for making and using a combinatorial library to identify modified thioaptamers that bind to, and affect the immune response of a host animal, transcription factors such as IL-6, NF-κB, AP-1 and the like. Composition and methods are also provided for the treatment of viral infections, as well as, vaccines and vaccine adjuvants are provided that modify host immune responses.

대표청구항 ▼

1. A system for identifying one or more proteins that interact with a specific oligonucleotide on a bead comprising: an oligonucleotide-bead library for binding one or more proteins, wherein each bead of the oligonucleotide-bead library comprises more than one copy of a unique oligonucleotide having a unique sequence, wherein the unique oligonucleotides are attached to the beads by a hexaethyleneglycol linker, and wherein the oligonucleotides attached to the bead are not released during phosphoroamidite deprotection; anda bead separator wherein the one or more beads of the bead library to which protein has bound are separated by the bead separator. 2. The system of claim 1, further comprising a nucleic acid sequencer to determine the sequence of the oligonucleotide. 3. The system of claim 1, wherein the sequence of the oligonucleotides on the one or more beads that bind to known proteins is determined. 4. The system of claim 1, wherein the one or more proteins bound to the oligonucleotide are extracted, separated and identified by liquid chromatography, mass spectrometry, or liquid chromatography and time of flight (TOF) mass spectrometry (MS) MALDI-TOF. 5. The system of claim 1, further comprising a mass spectrometer to identify the proteins that have bound to the one or more oligonucleotide-beads. 6. The system of claim 1, wherein the oligonucleotide is defined further as comprising an at least partially thio-modified oligonucleotide. 7. The system of claim 1, wherein the one or more proteins is further defined as being a protein extracted from a cell. 8. The system of claim 1, wherein the one or more proteins is further defined as being a protein extracted from a virally infected cell. 9. The system of claim 1, wherein the one or more proteins is further defined as being a protein extracted from a diseased cell. 10. The system of claim 1, wherein the bead library is substantially protein-free. 11. The system of claim 1, wherein the bead library comprises an [S]-oligonucleotide or an [S2]-oligonucleotide combinatorial bead library. 12. The system of claim 1, wherein the oligonucleotides of the bead library are single stranded. 13. The system of claim 1, wherein the oligonucleotides of the bead library comprises RNA. 14. The system of claim 1, wherein the bead library comprises sequence motifs for high affinity with cellular proteins selected from the group consisting essentially of proteins that are members of the AP-1, RBP-Jκ, NF-κB, NF IL-6 , CREB and GRE protein families, and combinations thereof.

이 특허에 인용된 특허 (74)

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IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

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연합인증

Structure based and combinatorially selected oligonucleoside phosphorothioate and phosphorodithioate aptamer targeting AP-1 transcription factors 원문보기

초록 ▼

대표청구항 ▼

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연합인증

Structure based and combinatorially selected oligonucleoside phosphorothioate and phosphorodithioate aptamer targeting AP-1 transcription factors 원문보기

초록 ▼

대표청구항 ▼

연구과제 타임라인

전체(0) 논문(0) 특허(0) 보고서(0)

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