초록 ▼

A vascular model includes at least one microchannel perfusable by at least one fluid and in a form of a hollow structure having a wall thickness in a range from 0.1 μm to 1000 μm, a concave inner surface with a cross-section which is circular in part and at least one pore having a diameter in a rang

A vascular model includes at least one microchannel perfusable by at least one fluid and in a form of a hollow structure having a wall thickness in a range from 0.1 μm to 1000 μm, a concave inner surface with a cross-section which is circular in part and at least one pore having a diameter in a range from 1 nm to 100 μm. The at least one microchannel has a width in a range from 0.01 μm to 10 mm and a depth in a range from 0.01 μm to 10 mm. At least one chamber is perfusable by a same and/or different fluid and surrounds the at least one microchannel over an entire length and width or over parts thereof. The at least one chamber adjoins the at least one microchannel. At least one connector is configured to receive and/or discharge the at least one fluid.

대표청구항 ▼

1. A vascular model comprising: at least one man-made chamber including a cover and a base plate, wherein the cover and base plate are formed of a plastic material film or of glass discs,wherein the at least one man-made chamber is perfusable by at least one of a first fluid and a second fluid;at le

1. A vascular model comprising: at least one man-made chamber including a cover and a base plate, wherein the cover and base plate are formed of a plastic material film or of glass discs,wherein the at least one man-made chamber is perfusable by at least one of a first fluid and a second fluid;at least one microchannel in the form of a hollow channel structure, wherein the at least one microchannel is formed of a thin film wall made of a thermoplastic polymer,wherein the thin film wall of the microchannel has a thickness in a range from 0.1 μm to 1000 μm and has at least one pore having a diameter in a range from 1 nm to 100 μm,the at least one microchannel being at least partially surrounded by the at least one man-made chamber,wherein the at least one microchannel is perfusable by at least the first fluid,wherein at least a portion of the at least one microchannel is bonded directly to the cover of the man-made chamber along the length of the microchannel, such that the portion of the microchannel bonded to the cover has a semi-circular cross-section,the portion of the at least one microchannel having a semi-circular cross-section having a width in a range from 0.01 μm to 10 mm and a depth in a range from 0.01 μm to 10 mm;at least one first connector in fluidic communication with a lumen of the at least one microchannel and configured to at least one of receive or discharge the at least one first fluid; anda first pump system connected to the lumen of the at least one microchannel via the at least one first connector. 2. The vascular model according to claim 1, wherein the at least one microchannel is straight. 3. The vascular model according to claim 1, wherein the at least one microchannel includes at least one division into at least two branches. 4. The vascular model according to claim 1, wherein the at least one microchannel has a radius of curvature in a rage from 0.01 μm to 10000 μm. 5. The vascular model according to claim 1, wherein the at least one man-made chamber comprises at least two man-made chambers, and wherein at least a second man-made chamber surrounds a first man-made chamber which at least partially surrounds the at least one microchannel in such a way that a nested structure is formed. 6. The vascular model according to claim 1, wherein the at least one man-made chamber comprises at least two man-made chambers, and wherein each of the at least two man-made chambers surrounds different subregions of the at least one microchannel. 7. The vascular model according to claim 1, further comprising a layer of three-dimensionally cultured cells located on an exterior surface of the portion of microchannel having the semi-circular cross-section. 8. The vascular model according to claim 7, further comprising a medium reservoir, wherein the first pump system is configured to supply, via the lumen of the at least one microchannel, medium from the medium reservoir to the three-dimensionally cultured cells on the exterior of the microchannel, andwherein the cells are selected from the group consisting of endothelial cells, organ-specific cells, fibroblasts, and suspended cells. 9. The vascular model according to claim 1, further comprising an endothelial cell culture disposed on the inner surface of the at least one microchannel. 10. The vascular model according to claim 1, wherein one or more of the at least one microchannel, the cover, and the base plate are transparent. 11. The vascular model according to claim 1, wherein the cover is bonded to at least a portion of the at least one microchannel by thermal bonding. 12. The vascular model according to claim 1, wherein the cover is bonded directly to the hollow channel structure along a length of the hollow channel structure that extends in a direction of fluid flow through the microchannel. 13. The vascular model according to claim 1, further comprising: at least one second connector in fluidic communication with the at least one man-made chamber and configured to populate the man-made chamber with cell cultures and at least one of receive or discharge the first fluid or the second fluid; anda second pump system connected to the at least one man-made chamber via the at least one second connector. 14. The vascular model according to claim 13, wherein the first pump system connected to the lumen microchannel is configured to perfuse the first fluid into the lumen of the at least one microchannel to form a first circulation, wherein the second pump system connected to the at least one man-made chamber via the at least one second connector is configured to perfuse the second fluid into the at least one man-made chamber to form a second circulation, andwherein the first circulation and the second circulation are mutually independent. 15. The vascular model according to claim 1, wherein the semi-circular cross-section of the at least one microchannel is linear in a region where the cover is bonded to the hollow channel structure and is curved elsewhere. 16. The vascular model according to claim 1, wherein the thin film wall has a thickness in a range of from 0.1 μm to 100 μm. 17. A method for producing a vascular model, comprising: providing at least one man-made chamber including a cover and a base plate, wherein the cover and base plate are formed of a plastic material film or of glass discs,wherein the at least one man-made chamber is perfusable by at least one of a first fluid and a second fluid;producing at least one microchannel in the form of a hollow channel structure from a thin film wall material made of thermoplastic polymer, wherein the thin film wall material has a thickness in a range from 0.1 μm to 1000 μm, andwherein at least one of:the thin film wall material already comprises at least one pore having a diameter in a range of from 1 nm to 100 μm prior to forming the microchannel, orat least one pore having a diameter in a range from 1 nm to 100 μm is made in the thin film wall material or produced in the thin film wall material after forming the microchannel;placing at least a portion of the at least one microchannel into the at least one man-made channel;bonding at least a portion of the at least one microchannel directly to the cover of the at least one man-made chamber along a length of the at least one microchannel, such that the portion of the microchannel bonded to the cover has a semi-circular cross-section, wherein the portion of the at least one microchannel having a semi-circular cross-section has a width in a range from 0.01 μm to 10 mm and a depth in a range from 0.01 μm to 10 mm;providing a first pump system; andcoupling the first pump system to a lumen of the at least one microchannel. 18. A method of using a vascular model, comprising: providing the vascular model of claim 1; andculturing tubular tissue structures on at least one surface of the at least one microchannel or inside the at least one chamber. 19. The method according to claim 18, wherein at least one of blood vessels and lymphatic vessels, the blood-brain barrier, lung, gastric or intestinal epithelia, or glandular structure are imitated.