[특허]Method of isolating cells from a tissue in a mammal

Method of isolating cells from a tissue in a mammal 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	C12N-005/071 A01N-001/02 A61K-038/42 A61K-038/48 A61K-035/39
출원번호	US-0755091 (2013-01-31)
등록번호	US-9657271 (2017-05-23)
발명자 / 주소	Oberholzer, Jose Doubleday, Marc
출원인 / 주소	The Board of Trustees of the University of Illinois
대리인 / 주소	Hamilton, Brook, Smith & Reynolds, P.C.
인용정보	피인용 횟수 : 0 인용 특허 : 85

초록 ▼

Solutions and suspensions comprising polymerized hemoglobin derived from human blood are disclosed. The solutions and suspensions may comprise cell culture medium, an enzyme (such as a protease), and/or a buffer. Processes of preparing the solutions and suspensions are also disclosed. The solutions and suspensions may be employed in methods of isolating mammalian cells, such as pancreatic islets, methods of preserving mammalian tissue and organs, methods of aiding the recovery of mammalian cells following their isolation, methods of maintaining mammalian cells, methods of propagating mammalian cells, and methods of treating a mammal with diabetes.

대표청구항 ▼

1. A method of isolating pancreatic islets from a pancreatic tissue in a mammal, comprising the steps of: a) contacting the pancreatic tissue of the mammal that includes the pancreatic islets a solution that includes polymerized hemoglobin derived from mammalian blood and a proteolytic enzyme; wherein the solution includes a concentration of between 5 and 15 g/dL of polymerized hemoglobin; and thereafterb) recovering the pancreatic islets from the mammalian tissue, thereby isolating the pancreatic islets. 2. The method of claim 1, wherein the tissue is human pancreatic tissue and the polymerized hemoglobin is derived from human blood. 3. The method of claim 2, wherein the polymerized hemoglobin derived from human blood is pyridoxylated. 4. The method of claim 2, wherein the solution is oxygenated. 5. The method of claim 2, wherein the contacting step is achieved by intraductal administration. 6. The method of claim 2, further including the step of washing the pancreatic islets with a cell culture medium to produce washed pancreatic islets. 7. The method of claim 6, further including the step of purifying the washed pancreatic islets by density gradient centrifugation. 8. The method of claim 1, wherein the solution further includes a cell culture medium. 9. The method of claim 8, wherein the cell culture medium is RPMI. 10. The method of claim 1, wherein the proteolytic enzyme is collagenase. 11. The method of claim 1, wherein the solution further includes a buffer. 12. The method of claim 1, wherein the polymerized hemoglobin is derived from human blood and is pyridoxylated. 13. The method of claim 12, wherein the solution further includes a cell culture medium. 14. The method of claim 13, wherein the proteolytic enzyme is collagenase and the cell culture medium is RPMI. 15. The method of claim 14, wherein the solution is oxygenated. 16. The method of claim 1, wherein the solution is oxygenated.

이 특허에 인용된 특허 (85)

Sehgal Lakshman R. (Cook County IL) De Woskin Richard E. (Cook County IL) Moss Gerald S. (Lake County IL) Gould Steven A. (Lake County IL) Rosen Arthur L. (Cook County IL) Sehgal Hansa (Cook County I, Acellular red blood cell substitute.
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IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

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연합인증

Method of isolating cells from a tissue in a mammal 원문보기

초록 ▼

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연구과제 타임라인

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연합인증

Method of isolating cells from a tissue in a mammal 원문보기

초록 ▼

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연구과제 타임라인

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