[미국특허]
Isolated polypeptides, polynucleotides useful for modifying water user efficiency, fertilizer use efficiency, biotic/abiotic stress tolerance, yield and biomass in plants
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A01H-005/00
C12N-015/82
C12N-015/29
A01H-005/10
C07K-014/415
출원번호
US-0867185
(2013-04-22)
등록번호
US-9670501
(2017-06-06)
발명자
/ 주소
Ronen, Gil
Vinocur, Basia Judith
Diber, Alex
Ayal, Sharon
Karchi, Hagai
Herschkovitz, Yoav
출원인 / 주소
Evogene Ltd.
인용정보
피인용 횟수 :
0인용 특허 :
56
초록▼
Polynucleotides, polypeptides, plant cells expressing same and methods of using same for increasing abiotic stress tolerance water use efficiency (WUE), fertilizer use efficiency (FUE), biomass, vigor and/or yield of a plant. The method is effected by expressing within the plant an exogenous polynuc
Polynucleotides, polypeptides, plant cells expressing same and methods of using same for increasing abiotic stress tolerance water use efficiency (WUE), fertilizer use efficiency (FUE), biomass, vigor and/or yield of a plant. The method is effected by expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising an amino acid sequence at least 80% homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065 and 3067-3259, thereby increasing the water use efficiency (WUE), the fertilizer use efficiency (FUE), the biomass, the vigor and/or the yield of the plant.
대표청구항▼
1. A method of increasing salinity stress tolerance, biomass, growth rate and/or yield of a plant as compared to a non-transformed plant under the same growth conditions, comprising: expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising the amino acid sequence set
1. A method of increasing salinity stress tolerance, biomass, growth rate and/or yield of a plant as compared to a non-transformed plant under the same growth conditions, comprising: expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising the amino acid sequence set forth by SEQ ID NO: 33,thereby increasing the salinity stress tolerance, biomass, growth rate and/or yield of the plant as compared to the non-transformed plant under the same growth conditions. 2. A method of producing a crop comprising growing a crop of a plant expressing an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide comprising the amino acid sequence set forth by SEQ ID NO: 33, wherein said plant is a progeny of a parent plant selected for increased yield, increased growth rate, increased biomass, and/or increased tolerance to salinity stress as compared to a control plant under the same growth conditions, and wherein said parent plant expresses said exogenous polynucleotide, thereby producing the crop. 3. The method of claim 1, wherein said polynucleotide is set forth by SEQ ID NO: 7 or SEQ ID NO: 2757. 4. The method of claim 1, further comprising growing the plant expressing said exogenous polynucleotide under the salinity stress. 5. A transgenic plant cell comprising a nucleic acid construct, said nucleic acid construct comprising an isolated polynucleotide comprising a nucleic acid sequence encoding a polypeptide exhibiting at least 97% global sequence identity to the entire amino acid set forth by SEQ ID NO:33, and a promoter for directing transcription of said nucleic acid sequence in a plant cell, wherein said polypeptide comprises a major intrinsic protein domain, is a water channel and is capable of increasing salinity stress tolerance, biomass, growth rate and/or yield of a plant as compared to a non-transformed plant under the same growth conditions, and wherein said promoter is heterologous to said transgenic plant cell. 6. The transgenic plant cell of claim 5, wherein said plant cell forms a part of a plant. 7. A method of increasing salinity stress tolerance, biomass, growth rate and/or yield of a plant as compared to a non-transformed plant under the same growth conditions, comprising: expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising an amino acid sequence exhibiting at least 97% global sequence identity to the entire amino acid sequence set forth by SEQ ID NO: 33, wherein said polypeptide comprises a major intrinsic protein domain, is a water channel and is capable of increasing salinity stress tolerance, biomass, growth rate and/or yield of a plant,thereby increasing the salinity stress tolerance, biomass, growth rate and/or yield of the plant as compared to the non-transformed plant under the same growth conditions. 8. The method of claim 2, wherein said polynucleotide is set forth by SEQ ID NO: 7 or SEQ ID NO: 2757. 9. The method of claim 7, wherein said increasing said biomass and/or said growth rate is performed under non-stress growth conditions. 10. A nucleic acid construct, comprising an isolated polynucleotide comprising a nucleic acid sequence encoding polypeptide exhibiting at least 97% sequence identity to the amino acid set forth by SEQ ID NO:33, and a promoter for directing transcription of said nucleic acid sequence in a plant cell, wherein said polypeptide comprises a major intrinsic protein domain, is a water channel and is capable of increasing salinity stress tolerance, biomass, growth rate and/or yield of a plant as compared to a non-transformed plant under the same growth conditions, and wherein said promoter is heterologous relative to said isolated polynucleotide. 11. A transgenic plant cell comprising the nucleic acid construct of claim 10. 12. A method of growing a crop, the method comprising seeding seeds and/or planting plantlets of a progeny of a parent plant, said parent plant has been transformed with the nucleic acid construct of claim 10, expresses said nucleic acid sequence encoding said polypeptide comprised in the nucleic acid construct of claim 10, and has been selected for at least one trait selected from the group consisting of: increased salinity stress tolerance, increased biomass, increased growth rate, and increased yield as compared to a non-transformed plant under the same growth conditions, wherein said seeds and/or said plantlets of said progeny comprise the nucleic construct of claim 10, and wherein said progeny has identical traits and characteristics of said parent plant, thereby growing the crop. 13. A method of increasing salinity stress tolerance, biomass, growth rate and/or yield of a plant as compared to a non-transformed plant under the same growth conditions, comprising transforming the plant with the nucleic acid construct of claim 10, thereby increasing the salinity stress tolerance, biomass, growth rate and/or yield of the plant as compared to the non-transformed plant under the same growth conditions. 14. The nucleic acid construct of claim 10, wherein said polypeptide is a plant polypeptide. 15. The nucleic acid construct of claim 10, wherein said nucleic acid sequence is selected from the group consisting of SEQ ID NOs: 2757, and 7, or a codon optimized sequence thereof. 16. The nucleic acid construct of claim 10, wherein said polypeptide exhibits at least 98% sequence identity to the amino acid set forth by SEQ ID NO:33. 17. The nucleic acid construct of claim 10, wherein said polypeptide exhibits at least 99% sequence identity to the amino acid set forth by SEQ ID NO:33. 18. The nucleic acid construct of claim 10, wherein said polypeptide is set forth by SEQ ID NO: 33. 19. A transgenic plant comprising the nucleic acid construct of claim 10. 20. The method of claim 13, wherein said nucleic acid sequence is selected from the group consisting of SEQ ID NOs: 2757, and 7, or a codon optimized sequence thereof. 21. The method of claim 13, wherein said polypeptide is set forth by SEQ ID NO: 33. 22. The method of claim 12, wherein said polypeptide is set forth by SEQ ID NO: 33.
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