Methods and compositions for exosome isolation
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C07K-001/30
C12Q-001/68
G01N-001/34
G01N-033/50
출원번호
US-0142143
(2016-04-29)
등록번호
US-9671321
(2017-06-06)
발명자
/ 주소
Vlassov, Alexander
Li, Mu
Zeringer, Emily
Conrad, Richard
출원인 / 주소
Life Technologies Corporation
인용정보
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초록▼
Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractiona
Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.
대표청구항▼
1. A method for the isolation of exosomes from a biological fluid sample comprising: a) adding a volume-excluding polymer to the biological fluid sample,b) incubating the biological fluid sample with the volume-excluding polymer;c) isolating the precipitated exosomes from the biological fluid sample
1. A method for the isolation of exosomes from a biological fluid sample comprising: a) adding a volume-excluding polymer to the biological fluid sample,b) incubating the biological fluid sample with the volume-excluding polymer;c) isolating the precipitated exosomes from the biological fluid sample; andd) fractionating the isolated exosomeswherein the volume-excluding polymer is polyethylene glycol, dextran, dextran sulfate, dextran acetate, polyvinyl alcohol, polyvinyl acetate, or polyvinyl sulfate; andwherein the molecular weight of the volume-excluding polymer is from 3000 to 10000 daltons. 2. The method of claim 1, wherein the concentration of volume-excluding polymer upon mixing with the biological fluid is from 3% to 15%. 3. The method of claim 1, further comprising the step of clarifying the biological fluid sample before adding the volume-excluding polymer. 4. The method of claim 1 further comprising the step of isolating nucleic acid and protein from the exosomes. 5. The method of claim 1, further comprising contacting the biological fluid sample prior to the addition of the volume-excluding polymer with a protease, under conditions suitable for digestion of protein. 6. The method of claim 5, wherein the protease is proteinase K. 7. A method for the isolation of exosomes from a biological tissue sample comprising: a) lysing the biological tissue sample,b) clarifying the lysed samplec) adding a volume-excluding polymer to the clarified sample,d) incubating the clarified sample with the volume-excluding polymer, ande) isolating the precipitated exosomeswherein the volume-excluding polymer is polyethylene glycol, dextran, dextran sulfate, dextran acetate, polyvinyl alcohol, polyvinyl acetate, or polyvinyl sulfate; andwherein the molecular weight of the volume-excluding polymer is from 3000 to 8000 daltons. 8. The method of claim 7, wherein the concentration of volume-excluding polymer upon mixing with the biological sample is from 3% to 15%. 9. The method of claim 7 further comprising the step of isolating nucleic acid and protein from the exosomes. 10. The method of claim 7, further comprising contacting the biological tissue sample with a protease, under conditions suitable for digestion of protein, prior to the addition of the volume-excluding polymer. 11. A kit for the isolation of exosomes comprising: a) one or more vessels containing one or more volume-excluding polymers, one or more buffers or one or more solutions for performing density gradient centrifugation of exosomes,b) one or more vessels containing antibodies or other ligands which bind a surface marker exposed on the surface of the exosome, andc) one or more solid supports which bind directly or indirectly to the exosomeswherein the volume-excluding polymer is polyethylene glycol, dextran, dextran sulfate, dextran acetate, polyvinyl alcohol, polyvinyl acetate, or polyvinyl sulfate; andwherein the molecular weight of the volume-excluding polymer is from 3000 to 8000 daltons. 12. The kit of claim 11, wherein the surface marker is selected from the group consisting of HLA DP haplotypes, HLA DQ haplotypes, HLA DR haplotypes, CD9, CD81, CD63 and CD82. 13. The kit of claim 11, wherein the solid support is a magnetic particle. 14. The kit of claim 11, further comprising a filtration device for separating exosomes by their size. 15. The method of claim 1 wherein the biological fluid sample is blood serum. 16. The method of claim 1 wherein the biological fluid sample is conditioned media. 17. The method of claim 1 wherein the biological fluid sample is urine. 18. The method of claim 1 wherein the biological fluid sample is amniotic fluid. 19. The method of claim 1 wherein the biological fluid sample is cerebrospinal fluid. 20. The method of claim 1 wherein the biological fluid sample is plasma.
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