[특허]Immunoassays employing non-particulate chemiluminescent reagent

[미국특허] Immunoassays employing non-particulate chemiluminescent reagent 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	G01N-033/78 G01N-033/58
출원번호	US-0131120 (2016-04-18)
등록번호	US-9720004 (2017-08-01)
발명자 / 주소	Singh, Pratap Zheng, Yi Feng Geng, Liping Janzen, Roland Schelp, Carsten
출원인 / 주소	Siemens Healthcare Diagnostics Inc.
대리인 / 주소	Tymeson, Cynthia G.
인용정보	피인용 횟수 : 0 인용 특허 : 27

초록 ▼

Methods and reagents are disclosed for conducting assays. Embodiments of the present methods and reagents are concerned with chemiluminescent reagents for determining the presence and/or amount of an analyte in a sample suspected of containing the analyte. The reagent is non-particulate and comprises a binding partner for the analyte and a chemiluminescent composition comprising an olefinic compound and a metal chelate. In embodiments of an assay, a combination is provided that comprises a sample suspected of containing the analyte, a chemiluminescent reagent as described above and a sensitizer reagent capable of generating singlet oxygen. The combination is subjected to conditions for binding of the analyte to the binding partner for the analyte. The sensitizer is activated and the amount of luminescence generated by the chemiluminescent composition is detected wherein the amount of luminescence is related to the amount of the analyte in the sample.

대표청구항 ▼

1. A chemiluminescent reagent for determining the presence and/or amount of an analyte in a sample suspected of containing the analyte, the reagent being non-particulate and soluble in an aqueous medium and comprising a binding partner for the analyte and a chemiluminescent composition comprising an olefinic compound and a metal chelate. 2. The chemiluminescent reagent according to claim 1 wherein the binding partner for the analyte is covalently bound to the chemiluminescent composition by a bond or a linking group. 3. The chemiluminescent reagent according to claim 2 wherein the binding partner for the analyte is covalently bound to the chemiluminescent composition by a linking group and wherein the linking group comprises a hydrophilic macromolecule. 4. The chemiluminescent reagent according to claim 3 wherein the olefinic compound and the metal chelate are linked to the same molecule of the linking group to which the binding partner for the analyte is covalently bound. 5. The chemiluminescent reagent according to claim 3 wherein the olefinic compound and the metal chelate are linked to separate molecules of the linking group to which the binding partner for the analyte is covalently bound and wherein the linking group may be the same or different. 6. The chemiluminescent reagent according to claim 3 wherein the hydrophilic macromolecule is selected from the group consisting of polypeptides, dendrimers, polycarboxylates, polyamines, polysulfhydryls and polyethyleneglycols. 7. The chemiluminescent reagent according to claim 1 wherein the binding partner for the analyte is non-covalently bound to the chemiluminescent composition by a specific binding pair wherein one member of the specific binding pair is linked to the binding partner for the analyte and the other member of the specific binding pair is linked to the chemiluminescent composition by a bond or a linking group. 8. The chemiluminescent reagent according to claim 7 wherein the binding partner for the analyte is non-covalently bound to the chemiluminescent composition by a specific binding pair wherein one member of the specific binding pair is linked to the binding partner for the analyte and the other member of the specific binding pair is linked to the chemiluminescent composition by a linking group wherein the linking group is a protein. 9. The chemiluminescent reagent according to claim 8 wherein the olefinic compound and the metal chelate are linked to the same molecule of the linking group to which the binding partner for the analyte is non-covalently bound. 10. The chemiluminescent reagent according to claim 8 wherein the olefinic compound and the metal chelate are linked to separate molecules of the linking group to which the binding partner for the analyte is non-covalently bound and wherein the linking group may be the same or different. 11. The chemiluminescent reagent according to claim 3 wherein the hydrophilic macromolecule is a protein. 12. The chemiluminescent reagent according to claim 7 wherein the specific binding pair is selected from the group consisting of (i) small molecule and binding partner for the small molecule and (ii) large molecule and binding partner for the large molecule. 13. The chemiluminescent reagent according to claim 1 wherein the metal of the metal chelate is a rare earth metal or a metal of Group VIII. 14. The chemiluminescent reagent according to claim 1 wherein the metal is selected from the group consisting of europium, terbium, dysprosium, samarium osmium and ruthenium. 15. The chemiluminescent reagent according to claim 1 wherein the metal chelate comprises a chelating agent selected from the group consisting of NHA, BHHT, BHHCT, DPP, TTA, NPPTA, NTA, TOPO, TPPO, BFTA, 2,2-dimethyl-4-perfluorobutyoyl-3-butanone (fod), 2,2′-dipyridyl (bpy), bipyridylcarboxylic acid, aza crown ethers, aza cryptands and trioctylphosphine oxide and derivatives thereof. 16. The chemiluminescent reagent according to claim 1 wherein the olefinic compound is selected from the group consisting of thioxenes, dioxenes, enol ethers, enamines, 9-alkylidenexanthans, 9-alkylidene-N-alkylacridans, aryl vinyl ethers, arylimidazoles and lucigenin and derivatives thereof. 17. The chemiluminescent reagent according to claim 1 wherein the metal is europium, the chelating agent is BHHCT and the olefinic compound is a carboxyl derivative of thioxene.

이 특허에 인용된 특허 (27) 인용/피인용 타임라인 분석

Bose Matthias,DEX, Arrangement and method of examining hydrophilic macromolecules in an aqueous solution.
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Ullman Edwin F. ; Kirakossian Hrair ; Pease John S. ; Daniloff Yuri ; Wagner Daniel B., Assay method utilizing induced luminescence.
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Ullman Edwin F. ; Singh Sharat, Chemiluminescent compositions and their use in the detection of hydrogen peroxide.
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Sharat Singh ; Edwin F. Ullman, Chemiluminescent compositions for use in detection of multiple analytes.
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Singh Sharat (San Jose CA) Singh Rajendra (Mountain View CA) Meneghini Frank (Keene NH) Ullman Edwin F. (Atherton CA), Chemiluminescent compounds and methods of use.
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Ullman Edwin F. (Atherton CA) Schwarzberg Moshe (Palo Alto CA), Fluorescence quenching with immunological pairs in immunoassays.
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Ullman Edwin F. (Atherton CA) Schwarzberg Moshe (Palo Alto CA), Fluorescence quenching with immunological pairs in immunoassays.
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Ullman Edwin F. (Atherton CA) Schwarzberg Moshe (Sunnyvale CA), Fluorescence quenching with immunological pairs in immunoassays.
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Tashiro,Kei; Honjo,Tasuku; Ikegawa,Masaya; Matsumoto,Kazuko, High sensitivity immunoassay method.
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Weimer, Bart, Ligand-DNA composition for capture and detection of contaminants on a solid surface.
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Sharat Singh ; Edwin F. Ullman, Metal chelate containing compositions for use in chemiluminescent assays.
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Singh Sharat (San Jose CA) Ullman Edwin F. (Atherton CA), Metal chelate containing compositions for use in chemiluminescent assays.
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Singh Sharat ; Ullman Edwin F., Metal chelate containing compositions for use in chemiluminescent assays.
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Singh Sharat ; Ullman Edwin F., Metal chelate containing compositions for use in chemiluminescent assays.
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Singh, Sharat; Ullman, Edwin F., Metal chelate containing compositions for use in chemiluminescent assays.
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Singh, Sharat; Ullman, Edwin F., Metal chelate containing compositions for use in chemiluminescent assays.
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Singh,Sharat; Ullman,Edwin F., Metal chelate containing compositions for use in chemiluminescent assays.
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Singh, Sharat; Pease, John S.; Sadakian, Jacqueline; Wagner, Daniel B.; Ullman, Edwin F., Particles for diagnostic and therapeutic use.
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Singh,Sharat; Pease,John S.; Sadakian,Jacqueline; Wagner,Daniel B.; Ullman,Edwin F., Particles for diagnostic and therapeutic use.
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Pease John S. ; Kirakossian Hrair ; Wagner Daniel B. ; Ullman Edwin F., Photoactivatable chemiluminescent matrices.
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de Keczer, Steve; Liu, Yen Ping; Davalian, Dariush; Kurn, Nurith; Ullman, Edwin F., Protecting groups for biological labeling.
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Patel, Rajesh; Kurn, Nurith, Quantitative determination of nucleic acid amplification products.
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Reinhold B. Pollner, Real-time monitoring of PCR using LOCI.
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Ullman, Edwin F.; Pirio, Marcel; Ericson, Mary C.; Wagner, Daniel B.; Davalian, Dariush, Simultaneous screening of multiple analytes.
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Ullman,Edwin F.; Pirio,Marcel; Ericson,Mary C.; Wagner,Daniel B.; Davalian,Dariush, Simultaneous screening of multiple analytes.
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Ullman,Edwin F.; Pirio,Marcel; Ericson,Mary C.; Wagner,Daniel B.; Davalian,Dariush, Simultaneous screening of multiple analytes.
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Wunderlich Jens-Christian,DEX ; Schick Ursula,DEX ; Werry Jurgen,DEX ; Freidenreich Jurgen,DEX, Solid and liquid solutions of sparingly water-soluble medicinal substances.
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[미국특허] Immunoassays employing non-particulate chemiluminescent reagent 원문보기