Biological production of multi-carbon compounds from methane
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12P-007/16
C12N-009/10
C12N-009/88
C12N-015/52
C12N-009/04
출원번호
US-0192290
(2016-06-24)
등록번호
US-9745603
(2017-08-29)
발명자
/ 주소
Coleman, William J.
Vidanes, Genevieve M.
Cottarel, Guillaume
Muley, Sheela
Kamimura, Roy
Javan, Akbar F.
Sun, Jianping
Groban, Eli S.
출원인 / 주소
Intrexon Corporation
대리인 / 주소
Troutman Sanders LLP
인용정보
피인용 횟수 :
0인용 특허 :
23
초록▼
Multi-carbon compounds such as ethanol, n-butanol, sec-butanol, isobutanol, tert-butanol, fatty (or aliphatic long chain) alcohols, fatty acid methyl esters, 2,3-butanediol and the like, are important industrial commodity chemicals with a variety of applications. The present invention provides metab
Multi-carbon compounds such as ethanol, n-butanol, sec-butanol, isobutanol, tert-butanol, fatty (or aliphatic long chain) alcohols, fatty acid methyl esters, 2,3-butanediol and the like, are important industrial commodity chemicals with a variety of applications. The present invention provides metabolically engineered host microorganisms which metabolize methane (CH4) as their sole carbon source to produce multi-carbon compounds for use in fuels (e.g., bio-fuel, bio-diesel) and bio-based chemicals. Furthermore, use of the metabolically engineered host microorganisms of the invention (which utilize methane as the sole carbon source) mitigate current industry practices and methods of producing multi-carbon compounds from petroleum or petroleum-derived feedstocks, and ameliorate much of the ongoing depletion of arable food source “farmland” currently being diverted to grow bio-fuel feedstocks, and as such, improve the environmental footprint of future bio-fuel, bio-diesel and bio-based chemical compositions.
대표청구항▼
1. A genetically modified methanotroph that is capable of producing 2,3-butanediol from methane (CH4), the genetically modified methanotroph comprising exogenous polynucleotides encoding acetolactate synthase and 2,3-butanediol dehydrogenase, wherein the acetolactate synthase has acetolactate syntha
1. A genetically modified methanotroph that is capable of producing 2,3-butanediol from methane (CH4), the genetically modified methanotroph comprising exogenous polynucleotides encoding acetolactate synthase and 2,3-butanediol dehydrogenase, wherein the acetolactate synthase has acetolactate synthase activity and comprises an amino acid sequence having at least 90% sequence homology to SEQ ID NO:2, and the 2,3-butanediol dehydrogenase has 2,3-butanediol dehydrogenase activity and comprises an amino acid sequence having at least 90% sequence homology to SEQ ID NO: 157, wherein said methanotroph is capable of converting methane to pyruvate through an endogenous type I RuMP pathway or a type II serine pathway. 2. The genetically modified methanotroph of claim 1, wherein said methanotroph is Methylobacter, Methylomicrobium, Methylomonas, Methylocaldum, Methylococcus, Methylosoma, Methylosarcina, Methylothermus, Methylohalobius, Methylogaea, Methylovulum, Crenothrix, Clonothrix, Methylosphaera, Methylocapsa, Methylocella, Methylosinus, Methylocystis, or Methyloacidophilum. 3. The genetically modified microorganism of claim 2, wherein said methanotroph is Methylococcus. 4. The genetically modified microorganism of claim 3, wherein said Methylococcus is Methylococcus capsulatus. 5. A method of producing 2,3-butanediol from methane comprising: (a) contacting a methanotroph with methane,(b) growing said methanotroph under suitable growth conditions to produce said 2,3-butanediol, wherein said methanotroph is capable of converting methane to pyruvate through an endogenous type I RuMP pathway or a type II serine pathway and comprises exogenous polynucleotides encoding acetolactate synthase and 2,3-butanediol dehydrogenase, wherein the acetolactate synthase has acetolactate synthase activity and comprises an amino acid sequence having at least 90% sequence homology to SEQ ID NO:2, and the 2,3-butanediol dehydrogenase has 2,3-butanediol dehydrogenase activity and comprises an amino acid sequence having at least 90% sequence homology to SEQ ID NO: 157. 6. The method of claim 5, wherein said methanotroph is Methylobacter, Methylomicrobium, Methylomonas, Methylocaldum, Methylococcus, Methylosoma, Methylosarcina, Methylothermus, Methylohalobius, Methylogaea, Methylovulum, Crenothrix, Clonothrix, Methylosphaera, Methylocapsa, Methylocella, Methylosinus, Methylocystis, or Methyloacidophilum. 7. The method of claim 5, further comprising isolating and purifying said 2,3-butanediol. 8. The method of claim 6, wherein said methanotroph is Methylococcus. 9. The method of claim 8, wherein said Methylococcus is Methylococcus capsulatus.
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