초록 ▼

Methods for the synthesis and use of several variations of styrene maleic acid-based polymers and the hydrogel tissue bridges that can be formed from such polymers. Specifically, a method is disclosed for synthesizing a styrene maleic acid-based polymer that can be dissolved in DMSO and injected int

Methods for the synthesis and use of several variations of styrene maleic acid-based polymers and the hydrogel tissue bridges that can be formed from such polymers. Specifically, a method is disclosed for synthesizing a styrene maleic acid-based polymer that can be dissolved in DMSO and injected into the vasa deferentia of male subjects, creating a hydrogel tissue bridge. This hydrogel tissue bridge can occlude the vas deferens, thus forming an effective male contraceptive. Additionally, this male contraceptive can be reversed by injecting the lumen of the vas deferens with a basic buffer solution to dissolve and remove the hydrogel tissue bridge.

대표청구항 ▼

1. A method for using a hydrogel-forming solution to create a hydrogel tissue bridge within a space located within a subject, the method comprising: providing a hydrogel-forming solution comprising a polymer dissolved in a solvent, wherein the polymer is more than 75% comprised of styrene-alt-maleic

1. A method for using a hydrogel-forming solution to create a hydrogel tissue bridge within a space located within a subject, the method comprising: providing a hydrogel-forming solution comprising a polymer dissolved in a solvent, wherein the polymer is more than 75% comprised of styrene-alt-maleic acid and the solvent is DMSO;identifying a space within a subject comprising available water and aqueous solutions within the space;utilizing an injecting apparatus to place the hydrogel-forming solution within the space; andthe hydrogel-forming solution absorbing the available water and aqueous solutions within the space thereby creating a hydrogel tissue bridge within the space, wherein the space is the vas deferens; and further comprising removing the hydrogel tissue bridge by injecting a basic phosphate buffer solution into the vas deferens with an injecting apparatus. 2. The method as described in claim 1 wherein the injecting apparatus is a needle and syringe. 3. The method as described in claim 1 wherein the ratio of polymer to DMSO in the hydrogel-forming solution is within a weight/weight range from 18% to 40%. 4. The method as described in claim 1 wherein the molecular weight of the polymer is within a range between 100 kDa and 1200 kDa. 5. The method as described in claim 1 wherein a percentage of intermolecular or intramolecular cross-links in the polymer is less than one percent. 6. The method as described in claim 1 wherein a percentage of intermolecular or intramolecular cross-links in the polymer is less than five percent. 7. The method as described in claim 1 wherein a percentage of intermolecular or intramolecular cross-links in the polymer is less than ten percent. 8. A method for synthesizing a polymer that is more than 75% comprised of styrene-alt-maleic acid and has a molecular weight between 100 kDa and 1200 kDa, the method comprising: preparing a more than 75% pure styrene maleic anhydride in copolymerization steps involving selected amounts of styrene and maleic anhydride including employment of a solvent and the free-radical initiation, from the thus prepared, copolymerized, styrene maleic anhydride wherein the solvent employed is a selected amount of ethyl acetate, and free-radical initiation is implemented via the addition of a selected amount of benzoyl peroxide, wherein the molecular weight of the of the prepared styrene-alt-maleic acid is determined by the amount of benzoyl peroxide used in the preparation of the styrene maleic anhydride. 9. The method as described in claim 8, wherein the selected amount of maleic anhydride and the selected amount of styrene are dissolved in a selected amount of ethyl acetate to create an initial solution, and the initial solution is heated to approximately 67° C. before the selected amount of benzoyl peroxide is added.