In one aspect, the present invention provides a chromatographic stationary phase material for various different modes of chromatography represented by Formula 1: X(W)a(Q)b(T)c (Formula 1). X can be a high purity chromatographic core composition having a surface comprising a silica core material, met
In one aspect, the present invention provides a chromatographic stationary phase material for various different modes of chromatography represented by Formula 1: X(W)a(Q)b(T)c (Formula 1). X can be a high purity chromatographic core composition having a surface comprising a silica core material, metal oxide core material, an inorganic-organic hybrid material or a group of block copolymers thereof. W can be absent and/or can include hydrogen and/or can include a hydroxyl on the surface of X. Q can be a functional group that minimizes retention variation over time (drift) under chromatographic conditions utilizing low water concentrations. T can include one or more hydrophilic, polar, ionizable, and/or charged functional groups that chromatographically interact with the analyte. Additionally, b and c can be positive numbers, with the ratio 0.05≤(b/c)≤100, and a≥0.
대표청구항▼
1. A method for mitigating or preventing retention drift in supercritical fluid chromatography, sub-critical fluid chromatography, or carbon dioxide based chromatography comprising: chromatographically separating a sample using a chromatographic device comprising a chromatographic stationary phase r
1. A method for mitigating or preventing retention drift in supercritical fluid chromatography, sub-critical fluid chromatography, or carbon dioxide based chromatography comprising: chromatographically separating a sample using a chromatographic device comprising a chromatographic stationary phase represented by Formula 1: [X](W)a(Q)b(T)c Formula 1wherein:X is a chromatographic core composition having a surface comprising a silica core material, metal oxide core material, an inorganic-organic hybrid material, or a group of block copolymers thereof;W is an hydroxyl group on the surface of X;Q is represented by: T is represented by one of: wherein each Z group independently comprises: a) a surface attachment group having the formula (B1)x(R5)y(R6)zSi— wherein x is an integer from 1-3,y is an integer from 0-2,z is an integer from 0-2,and x+y+z=3each occurrence of R5 and R6 independently represents methyl, ethyl, n-butyl, iso-butyl, tert-butyl, iso-propyl, substituted or unsubstituted aryl, cyclic alkyl, branched alkyl, lower alkyl, a protected or deprotected alcohol, or a zwitterion group; andB1 represents a siloxane bond;b) an attachment to a surface organofunctional hybrid group selected from the group consisting of a direct carbon-carbon bond, a heteroatom linkage, ester linkage, ether linkage, thioether linkage, amine linkage, amide linkage, imide linkage, urea linkage, carbonate linkage, carbamate linkage, heterocycle linkage, triazole linkage and urethane linkage; orc) an adsorbed, surface group that is not covalently attached to the surface of X; and b and c are positive numbers, 0.05≤(b/c)≤100, and a≥0,thereby mitigating or preventing retention drift. 2. The method of claim 1, wherein T is represented by:
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이 특허에 인용된 특허 (11)
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