A method of modifying the content of certain bacteria in uncured tobacco material is provided, the method including contacting an uncured tobacco material with a treatment solution, wherein the treatment solution is selected from the group consisting of: (i) a solution comprising salt, sugar, or a c
A method of modifying the content of certain bacteria in uncured tobacco material is provided, the method including contacting an uncured tobacco material with a treatment solution, wherein the treatment solution is selected from the group consisting of: (i) a solution comprising salt, sugar, or a combination thereof; (ii) a solution comprising one or more enzymes; and (iii) a solution comprising one or more probiotics, wherein said contacting provides a treated tobacco material having a reduced total bacterial content following harvest. Also provided is a method of reducing TSNA content, the method including growing a tobacco plant in soil treated with a chloride source; harvesting the tobacco plant; and curing the harvested tobacco plant to give a cured tobacco material, providing a treated, cured tobacco material having a TSNA content that is reduced as compared to a cured tobacco material that has been grown in soil not treated with a chloride source. Smoking articles and smokeless tobacco products including such treated tobacco materials are also provided.
대표청구항▼
1. A method of reducing TSNA content of a tobacco material, comprising: growing a tobacco plant in soil treated with a chloride source;harvesting the tobacco plant; andcuring the harvested tobacco plant to give a cured tobacco material,wherein said method provides a treated, cured tobacco material h
1. A method of reducing TSNA content of a tobacco material, comprising: growing a tobacco plant in soil treated with a chloride source;harvesting the tobacco plant; andcuring the harvested tobacco plant to give a cured tobacco material,wherein said method provides a treated, cured tobacco material having a TSNA content that is reduced as compared to a cured tobacco material that has been grown in soil not treated with a chloride source, wherein the soil is treated with the chloride source in an amount of about 25 to about 150 lbs per acre. 2. The method of claim 1, further comprising planting tobacco prior to the soil treatment. 3. The method of claim 1, further comprising planting tobacco after the soil treatment. 4. The method of claim 1, further comprising applying the chloride source to the soil. 5. The method of claim 4, wherein the chloride source is a chloride salt. 6. The method of claim 4, wherein the chloride source is a compound capable of decomposition to provide chloride ions. 7. The method of claim 4, wherein the chloride source is selected from the group consisting of sodium chloride (NaCl), potassium chloride (KCl), magnesium chloride (MgCl2), calcium chloride (CaCl2), and combinations thereof. 8. The method of claim 4, wherein the chloride source is KCl. 9. The method of claim 1, wherein the chloride source comprises one or more fertilizer components, selected from the group consisting of nitrogen, potassium, and phosphorus. 10. The method of claim 4, wherein the applying comprises applying the chloride source in solid form to the soil. 11. The method of claim 4, wherein the applying comprises applying a solution of the chloride source to the soil. 12. The method of claim 1, wherein the combined content of NNN, NAT, NAB, and NAK in the treated, cured tobacco material is less than about 500 ng/g. 13. The method of claim 1, wherein the combined content of NNN, NAT, NAB, and NAK in the treated, cured tobacco material is less than about 200 ng/g. 14. The method of claim 1, wherein the NNN content in the treated, cured tobacco material is less than about 100 ng/g. 15. The method of claim 1, wherein the NNN content in the treated, cured tobacco material is less than about 50 ng/g. 16. The method of claim 1, wherein the NAT content in the treated, cured tobacco material is less than about 100 ng/g. 17. The method of claim 1, wherein the NAT content in the treated, cured tobacco material is less than about 75 ng/g. 18. The method of claim 1, wherein the combined NAT and NNN content in the treated, cured tobacco material is less than about 150 ng/g. 19. The method of claim 1, wherein the NNN content in the treated, cured tobacco material represents greater than a 50% reduction as compared with that of a cured tobacco material from a plant grown in soil with comparable levels of N, P, and K but without any chloride source added thereto. 20. The method of claim 1, wherein the NAT content in the treated, cured tobacco material represents greater than a 50% reduction as compared with that of a cured tobacco material from a plant grown in soil with comparable levels of N, P, and K but without any chloride source added thereto. 21. The method of claim 1, wherein the curing comprises air curing. 22. The method of claim 1, wherein the curing comprises fire curing. 23. The method of claim 1, further comprising: processing the treated, cured tobacco material to provide a processed tobacco material in a form suitable for incorporation in a tobacco product; andincorporating the processed tobacco material into a smokeless tobacco product. 24. The method of claim 23, wherein the processed tobacco material is in the form of cut filler. 25. The method of claim 23, wherein the processed tobacco material is in the form of a tobacco blend.
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이 특허에 인용된 특허 (46)
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