Microorganisms and methods for the coproduction 1,4-butanediol and gamma-butyrolactone
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12N-001/20
C12P-017/04
C12P-007/18
C12N-015/52
출원번호
US-0954487
(2015-11-30)
등록번호
US-9988656
(2018-06-05)
발명자
/ 주소
Sun, Jun
Burk, Mark J.
Burgard, Anthony P.
Osterhout, Robin E.
Niu, Wei
Trawick, John D.
Haselbeck, Robert
출원인 / 주소
Genomatica, Inc.
대리인 / 주소
Jones Day
인용정보
피인용 횟수 :
0인용 특허 :
96
초록▼
The invention provides non-naturally occurring microbial organisms comprising 1,4-butanediol (14-BDO) and gamma-butyrolactone (GBL) pathways comprising at least one exogenous nucleic acid encoding a 14-BDO and GBL pathway enzyme expressed in a sufficient amount to produce 14-BDO and GBL. The inventi
The invention provides non-naturally occurring microbial organisms comprising 1,4-butanediol (14-BDO) and gamma-butyrolactone (GBL) pathways comprising at least one exogenous nucleic acid encoding a 14-BDO and GBL pathway enzyme expressed in a sufficient amount to produce 14-BDO and GBL. The invention additionally provides methods of using such microbial organisms to produce 14-BDO and GBL.
대표청구항▼
1. A non-naturally occurring microbial organism comprising exogenous nucleic acids encoding a combination of enzymes of: a 4-hydroxybutyrate dehydrogenase; a 4-hydroxybutyryl-CoA transferase; a 4-hydroxybutyryl-CoA reductase (aldehyde forming); a 1,4-butanediol dehydrogenase; and a 4-hydroxybutyrate
1. A non-naturally occurring microbial organism comprising exogenous nucleic acids encoding a combination of enzymes of: a 4-hydroxybutyrate dehydrogenase; a 4-hydroxybutyryl-CoA transferase; a 4-hydroxybutyryl-CoA reductase (aldehyde forming); a 1,4-butanediol dehydrogenase; and a 4-hydroxybutyrate lactonase;wherein the exogenous nucleic acids are expressed in the microbial organism. 2. The non-naturally occurring microbial organism of claim 1, wherein the microbial organism further comprises exogenous nucleic acids encoding a second combination of enzymes selective from the group consisting of: (a) a succinyl-CoA transferase, a succinyl-CoA hydrolase, or a succinyl-CoA synthetase; and a succinyl-CoA reductase (aldehyde forming);(b) an alpha-ketoglutarate decarboxylase; and(c) a glutamate dehydrogenase or glutamate transaminase; a glutamate decarboxylase; and a 4-aminobutyrate dehydrogenase or a 4-aminobutyrate transaminase,wherein the exogenous nucleic acids encoding the second combination of enzymes are expressed in the microbial organism. 3. The non-naturally occurring microbial organism of claim 1, wherein at least one, two, three, four or five of the exogenous nucleic acids is a heterologous encoding nucleic acid. 4. The non-naturally occurring microbial organism of claim 1, wherein the exogenous nucleic acids are heterologous encoding nucleic acids. 5. The non-naturally occurring microbial organism of claim 1, wherein the microbial organism is a bacterium, yeast or fungus. 6. The non-naturally occurring microbial organism of claim 5, wherein the bacterium is selected from the group consisting of Escherichia coli, Klebsiella oxytoca, Anaerobiospirillum succiniciproducens, Actinobacillus succinogenes, Mannheimia succiniciproducens, Rhizobium etli, Bacillus subtilis, Corynebacterium glutamicum, Gluconobacter oxydans, Zymomonas mobilis, Lactococcus lactis, Lactobacillus plantarum, Streptomyces coelicolor, Clostridium acetobutylicum, Pseudomonas fluorescens, and Pseudomonas putida. 7. The non-naturally occurring microbial organism of claim 5, wherein the yeast or fungus is selected from the group consisting of Schizosaccharomyces pombe, Kluyveromyces lactis, Kluyveromyces marxianus, Aspergillus terreus, Aspergillus niger, Pichia pastoris, Rhizopus arrhizus, and Rhizobus oryzae. 8. A composition comprising the non-naturally occurring microbial organism of claim 1 in a substantially anaerobic culture medium.
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